Green Synthesis,Chemical Characterization,and Antiproliferative Effect of Gold Nanoparticles Using Methanolic Extracts of Chrysophyllum cainito L. Peels
Restricted accessResearch articleFirst published online July, 2026
Green Synthesis,Chemical Characterization,and Antiproliferative Effect of Gold Nanoparticles Using Methanolic Extracts of Chrysophyllum cainito L. Peels
The article’s aim was thus to evaluate the antiproliferative activity of gold nanoparticles associated with the methanolic extract of Chrysophyllum cainito in human breast cancer cell line (MCF-7), cervical cancer tissue (HeLa), and colorectal cancer cell line (HCT-116) cancer cell lines. The methanolic extract showed the presence of polyphenols and organic acids related to anticancer properties. In relation to the gold nanoparticles functionalized with the methanolic extract of C. cainito peels, these nanoparticles showed significant cytotoxic activity (p = .0001) in the HCT-116 cell line at 72 h after exposure to these. We consider this the first contribution to the use of the methanolic extract of C. cainito for the green synthesis of gold nanoparticles. In conclusion, the green synthesis of gold nanoparticles associated with C. cainito methanolic peel extract may be an interesting option for the search for new cancer treatments.
Moo-HuchinVM, Estrada-MotaI, Estrada-LeónR, et al.Determination of some physicochemical characteristics, bioactive compounds and antioxidant activity of tropical fruits from Yucatan, Mexico. Food Chem, 2014; 152:508–515; doi: 10.1016/j.foodchem.2013.12.013
4.
Chel-GuerreroLD, Sauri-DuchE, Fragoso-SerranoMC, et al.Phytochemical profile, toxicity, and pharmacological potential of peels from four species of tropical fruits. J Med Food, 2018; 21(7):734–743; doi: 10.1089/jmf.2017.0124
5.
MeiraNA, KleinLC, RochaLW, et al.Anti-inflammatory and anti-hypersensitive effects of the crude extract, fractions and triterpenes obtained from Chrysophyllum cainito leaves in mice. J Ethnopharmacol, 2014; 151(2):975–983; doi: 10.1016/j.jep.2013.12.014
6.
MaoLM, QiXW, HaoJH, et al.In vitro, ex vivo and in vivo anti-hypertensive activity of Chrysophyllum cainito L. extract. Int J Clin Exp Med, 2015; 8(10):17912–17921.
7.
Marín-PeñalverJJ, Martín-TimónI, Sevillano-CollantesC, et al.Update on the treatment of type 2 diabetes mellitus. World J Diabetes, 2016; 7(17):354–395; doi: 10.4239/wjd.v7.i17.354
8.
KootiW, FarokhipourM, AsadzadehZ, et al.The role of medicinal plants in the treatment of diabetes: A systematic review. Electron Physician, 2016; 8(1):1832–1842; doi: 10.19082/1832
9.
SuryaS, SalamAD, TomyDV, et al.Diabetes mellitus and medicinal plants-a review. Asian Pac J Trop Dis, 2014; 4(5):337–347; doi: 10.1016/S2222-1808(14)60585-5
10.
LiL-B, LinS, YanJ, et al.Poly-phenolic fraction of Chrysophyllum cainito extract induces cell death in osteosarcoma cells. Bangladesh J Pharmacol, 2015; 10(4):972; doi: 10.3329/bjp.v10i4.24576
11.
DoanHV, LeTP. Chrysophyllum cainito: A tropical fruit with multiple health benefits. Evid Based Complement Alternat Med, 2020; 2020:7259267; doi: 10.1155/2020/7259267
12.
Arana-ArgáezVE, Chan-ZapataI, Canul-CancheJ, et al.Immunosuppresive effects of the methanolic extract of chrysophyllum cainito leaves on macrophage functions. Afr J Tradit Complement Altern Med, 2017; 14(1):179–186; doi: 10.21010/ajtcam.v14i1.20
13.
Rodríguez-LeónE, Rodríguez-VázquezBE, Martínez-HigueraA, et al.Synthesis of gold nanoparticles using mimosa tenuiflora extract, assessments of cytotoxicity, cellular uptake, and catalysis. Nanoscale Res Lett, 2019; 14(1):334; doi: 10.1186/s11671-019-3158-9
14.
OprisR, TatomirC, OlteanuD, et al.The effect of Sambucus nigra L. extract and phytosinthesized gold nanoparticles on diabetic rats. Colloids Surf B Biointerfaces, 2017; 150:192–200; doi: 10.1016/j.colsurfb.2016.11.033
15.
Al-YasiriAY, KhoobchandaniM, CutlerCS, et al.Mangiferin functionalized radioactive gold nanoparticles (MGF-(198)AuNPs) in prostate tumor therapy: Green nanotechnology for production, in vivo tumor retention and evaluation of therapeutic efficacy. Dalton Trans, 2017; 46(42):14561–14571; doi: 10.1039/c7dt00383h
16.
WangL, XuJ, YanY, et al.Green synthesis of gold nanoparticles from Scutellaria barbata and its anticancer activity in pancreatic cancer cell (PANC-1). Artif Cells Nanomed Biotechnol, 2019; 47(1):1617–1627; doi: 10.1080/21691401.2019.1594862
17.
WangL, XuJ, YanY, et al.Synthesis of gold nanoparticles from leaf Panax notoginseng and its anticancer activity in pancreatic cancer PANC-1 cell lines. Artif Cells Nanomed Biotechnol, 2019; 47(1):1216–1223; doi: 10.1080/21691401.2019.1593852
18.
MarySJ, VeeravarmalV, ThankappanP, et al.Anti-cancer effects of green synthesized gold nanoparticles using leaf extract of Annona muricata. L against squamous cell carcinoma cell line 15 through apoptotic pathway. Dent Res J (Isfahan), 2024; 21:14.
19.
SingletonVL, JosephA, Rossi. Colorimetry of Total Phenolics with Phosphomolybdic-Phosphotungstic Acid Reagents. Am J Enol Vitic, 1965; 16(3):144–158; doi: 10.5344/ajev.1965.16.3.144
20.
ChangC-C, YangM-H, WenH-M, et al.Estimation of total flavonoid content in propolis by two complementary colometric methods. J Food Drug Anal, 2002; 10(3):3.
21.
PellatiF, EpifanoF, ContaldoN, et al.Chromatographic methods for metabolite profiling of virus- and phytoplasma-infected plants of echinacea purpurea. J Agric Food Chem, 2011; 59(19):10425–10434; doi: 10.1021/jf2025677
22.
AarlandRC, Bañuelos-HernándezAE, Fragoso-SerranoM, et al.Studies on phytochemical, antioxidant, anti-inflammatory, hypoglycaemic and antiproliferative activities of Echinacea purpurea and Echinacea angustifolia extracts. Pharm Biol, 2017; 55(1):649–656; doi: 10.1080/13880209.2016.1265989
23.
FrensG. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature Physical Science, 1973; 241(105):20–22; doi: 10.1038/physci241020a0
24.
IeloI, RandoG, GiacobelloF, et al.Synthesis, Chemical–Physical Characterization, and Biomedical Applications of Functional Gold Nanoparticles: A Review. 2021.
25.
NuneSK, GundaP, ThallapallyPK, et al.Nanoparticles for biomedical imaging. Expert Opin Drug Deliv, 2009; 6(11):1175–1194; doi: 10.1517/17425240903229031
26.
XiaoM, ChenY, ZhengF, et al.Predicting the storage time of green tea by myricetin based on surface-enhanced Raman spectroscopy. NPJ Sci Food, 2023; 7(1):28; doi: 10.1038/s41538-023-00206-1
27.
ZłotekU, MikulskaS, NagajekM, et al.The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi J Biol Sci, 2016; 23(5):628–633; doi: 10.1016/j.sjbs.2015.08.002
28.
RababahTM, BanatF, RababahA, et al.Optimization of extraction conditions of total phenolics, antioxidant activities, and anthocyanin of oregano, thyme, terebinth, and pomegranate. J Food Sci, 2010; 75(7):C626–C632; doi: 10.1111/j.1750-3841.2010.01756.x
29.
BaronG, FerrarioG, MarinelloC, et al.Effect of extraction solvent and temperature on polyphenol profiles, antioxidant and anti-inflammatory effects of red grape skin by-product. Molecules, 2021; 26(18):5454; doi: 10.3390/molecules26185454
30.
LiuS, LinJ, WangC, et al.Antioxidant properties of various solvent extracts from lychee (Litchi chinenesis Sonn.) flowers. Food Chem, 2009; 114(2):577–581; doi: 10.1016/j.foodchem.2008.09.088
31.
MoureA, CruzJM, FrancoD, et al.Natural antioxidants from residual sources. Food Chem, 2001; 72(2):145–171; doi: 10.1016/S0308-8146(00)00223-5
32.
MadlenerS, IllmerC, HorvathZ, et al.Gallic acid inhibits ribonucleotide reductase and cyclooxygenases in human HL-60 promyelocytic leukemia cells. Cancer Lett, 2007; 245(1–2):156–162; doi: 10.1016/j.canlet.2006.01.001
33.
MotallebiM, BhiaM, RajaniHF, et al.Naringenin: A potential flavonoid phytochemical for cancer therapy. Life Sci, 2022; 305:120752; doi: 10.1016/j.lfs.2022.120752
34.
KopustinskieneDM, JakstasV, SavickasA, et al.Flavonoids as anticancer agents. Nutrients, 2020; 12(2):457; doi: 10.3390/nu12020457
35.
NadileM, RetsidouMI, GiotiK, et al.Resveratrol against cervical cancer: Evidence from in vitro and in vivo studies. Nutrients, 2022; 14(24):5273; doi: 10.3390/nu14245273
36.
HazafaA, RehmanKU, JahanN, et al.The role of polyphenol (Flavonoids) compounds in the treatment of cancer cells. Nutr Cancer, 2020; 72(3):386–397; doi: 10.1080/01635581.2019.1637006
37.
DoanHV, SritangosP, IyaraR, et al.Chrysophyllum cainito stem bark extract induces apoptosis in Human hepatocarcinoma HepG2 cells through ROS-mediated mitochondrial pathway. 2167-8359 (Print):2025.
38.
ChenF, QiS, ZhangX, et al.lncRNA PLAC2 Activated by H3K27 Acetylation Promotes Cell Proliferation and Invasion via the Activation of Wnt/β-catenin P-athway in O-ral S-quamous C-ell C-arcinoma. Int J Oncol 2019; 54(4):1183–1194; doi: 10.3892/ijo.2019.4707
39.
ZhaoB, HuM. Gallic acid reduces cell viability, proliferation, invasion and angiogenesis in human cervical cancer cells. Oncol Lett, 2013; 6(6):1749–1755; doi: 10.3892/ol.2013.1632
40.
Keyvani-GhamsariS, RahimiM, KhorsandiK. An update on the potential mechanism of gallic acid as an antibacterial and anticancer agent. Food Sci Nutr, 2023; 11(10):5856–5872; doi: 10.1002/fsn3.3615
41.
LinkS, El-SayedMA. Spectral properties and relaxation dynamics of surface plasmon electronic oscillations in gold and silver nanodots and nanorods. J Phys Chem B, 1999; 103(40):8410–8426; doi: 10.1021/jp9917648
42.
DongJ, CarpinonePL, PyrgiotakisG, et al.Synthesis of precision gold nanoparticles using Turkevich method. Kona, 2020; 37:224–232; doi: 10.14356/kona.2020011
43.
KumarI, MondalM, MeyappanV, et al.Green one-pot synthesis of gold nanoparticles using Sansevieria roxburghiana leaf extract for the catalytic degradation of toxic organic pollutants. Mater Res Bull, 2019; 117:18–27; doi: 10.1016/j.materresbull.2019.04.029
44.
ZhouY, LinW, HuangJ, et al.Biosynthesis of gold nanoparticles by foliar broths: Roles of biocompounds and other attributes of the extracts. Nanoscale Res Lett, 2010; 5(8):1351–1359; doi: 10.1007/s11671-010-9652-8
45.
ZhouL, LiuH, LiuK, et al.Gold compounds and the anticancer immune response. Front Pharmacol, 2021; 12:739481; doi: 10.3389/fphar.2021.739481
46.
GiordanoA, TommonaroG. Curcumin and Cancer. 2019.
47.
Vázquez-HernándezF, Mendoza-AcevedoS, Mendoza-BarreraCO, et al.Antibody-coupled hydroxyapatite nanoparticles as efficient tools for labeling intracellular proteins. Mater Sci Eng C Mater Biol Appl, 2017; 71:909–918; doi: 10.1016/j.msec.2016.10.082
48.
RónaváriA, KovácsD, IgazN, et al.Biological activity of green-synthesized silver nanoparticles depends on the applied natural extracts: A comprehensive study. Int J Nanomedicine, 2017; 12:871–883; doi: 10.2147/ijn.s122842
