HTS is a key component of pharmaceutical lead identification process. Over recent years, the pharmaceutical industry has experienced significant increases in the throughput capabilities of its HTS functions. In those companies where HTS has been effectively deployed, it is now possible to screen the entire corporate compound collection against a pharmacological target within a timescale of several weeks to a few months. This capability has been realized, not as a result of the purchase of any one particular piece of hardware, but rather through the development of a truly effective HTS infrastructure that matches the needs of the parent organization. Central to this is the need to understand how to effectively combine the use of the different types of hardware available to the HTS specialist. The use of both modular workstations and single-arm robotic systems have underpinned most HTS groups operations. Recent advances in the field of multiple-arm robotic systems and dedicated automation systems offer even further potential for increasing productivity. This article describes our experience with the use of a dedicated automation system for HTS applications.
References
1.
Beggs, M. (1997). Use of a two armed robot for high throughput screening. J. Biomolec. Screening2:71-78.
2.
Major, J.S. (1998). Challenges and opportunities in high throughput screening: Implications for new technologies. J. Biomolecular Screening3:13-17.
3.
Beggs, M. (1995). Automated compound preparation for high throughout screening. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 181-190.
4.
Aktin, S.C., Jamieson, R.B. (1995). Automated compound storage & retrieval systems Providing a Backbone for High Throughput Screening Programmes. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 681.
5.
Harrison, W. (1997). The importance of automated sample management systems in realising the potential of large compound libraries in drug discovery. J. Biomolec. Screening2:203-206.
6.
McCaffery, C., Powers, G., Kelly Talbot, M. (1996). Utilization of the ORCA to completely automate scintillation proximity assays. J. Biomolec. Screening1:187-190.
7.
Banks, M., Binnie, A., Fogarty, S. (1997). High throughput screening using fully integrated robotic screening. J. Biomolec. Screening2:133-135.
8.
Banks, M., Fogarty, S., Valler, M., Mills, K., O'Brien, S. (1996). Automated high throughput screening to aid the drug hunter at Glaxo Welcome. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 220-232.
9.
Donzel, A., Carmona, G., Corkan, L.A. (1997). Perspectives on scheduling. In High Throughput Screening: The Discovery of Bioactive Substances. Devlin, J.P. (ed.). Marcel Dekker Inc., pp. 525-545.
10.
Elands, J. (1995). An integrated and versatile system to rapidly determine potency, selectivity and potential for side effects: From assay automation and data management to result. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 159-168.
11.
Beggs, M. (1998). HTS assays: Developing assays for operation at high throughput. In Effective Assay development. IBC Conferences Proceedings, Frankfurt, Germany.
12.
Hecht, C. (1996). Automated Dissolution Testing in Pharmaceutical Development. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 543-551.
13.
Van Ausdall, D.A., Marshall, W.S. (1996). Automated high throughput mass spectral analysis of oligonucleotides. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 116-122.
14.
Campbell, J.B., Wildonger, R.A. (1996). Managing compound library production by parallel synthesis on automated workstation based systems. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 127-141.
15.
McCurley, J.P., Sekulovich, R. (1995). Developments and implementation of microplate immunoassays employing Zymark robotics. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 138-158.
16.
Palmer, M. (1995). High throughput screening for accelerated drug discovery. Proceedings of the International Symposium on Laboratory Automation and Robotics, Boston, MA, pp. 112-124.
17.
Alderman, E., Elands, J. (1998). Breaking a screening barrier. Genetic Eng. News18:14-15.
