Simultaneous 96-well pipetting is an integral procedure in high throughput screening (HTS). There are several automated 96-well pipettors in the marketplace today. Inherent to any successful HTS assay is an understanding of the limitations of the automated pipettor employed. When designing HTS protocols, two common concerns over artifacts being introduced into the data involve the accuracy and potential for tip carryover contamination by a 96-well pipettor. This report defines these parameters for the Tomtec Quadra 96-well pipettor. Included is a quantitative analysis on the pipetting accuracy at a range of commonly used volumes and the extent of tip carryover contamination before and after tip washing.
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Lindeburg, M.R., (1982). Engineer in Training Review Manual. Professional Publications, Inc., Belmont, CA.
