Abstract
AB SCIEX
TripleTOF 6600 System with SWATH Acquisition 2.0 and BioPharmaView Software
This high-resolution, accurate-mass system, combined with the new SWATH Acquisition 2.0, is for proteomic data challenges for advanced biomarker discovery and systems biology. The solution enables the quantification of thousands of proteins across large sample sets with data completeness and quantitative accuracy and precision.
AB SCIEX SWATH Acquisition incorporates a data-independent acquisition strategy and can fragment every detectable peptide in the sample to acquire quantitative MS/MS data. According to AB SCIEX, this approach has been shown to increase the capacity of targeted proteomics experiments by more than 30-fold and reduce study time by as much as 90%. The increased dynamic range of the TripleTOF 6600 System allows deeper interrogation of complex samples, and variable window acquisition and the SWATH 2.0 processing software improve quantitative results.
According to AB SCIEX, its Bio-PharmaView Software automates core workflows with investigative capabilities and fast data acquisition for the characterization of biotherapeutics such as monoclonal antibodies and emerging protein classes at both the intact protein and peptide levels. The software can efficiently analyze and compare batches while monitoring critical quality attributes according to the Food and Drug Administration’s well-characterized biotherapeutic product model.
More: www.absciex.com
AMSBIO
CRISPR/Cas9 Full Gene Editing Tool
Designed to produce a genetically modified cell using any mammalian cell line and targeting any gene without introducing foreign DNA. According to AMSBIO, the benefits of CRISPR/Cas9 over previous forms of gene editing are that it is much simpler to implement and has higher efficiency at performing biallelic gene modifications. Using CRISPPR/ Cas9 technology, it is now possible to create knock-in, knock-outs and mutations of any gene in any cell line.
CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible and simple system, resulting in high specificity and low cell toxicity. The CRISPR/Cas9 genome editing system requires the co-expression of a Cas9 protein with a guide RNA vector expressed from the human U6 polymerase III promoter. With the protospacer-adjacent motif (PAM; the sequence NGG) present at the 3′ end, Cas9 will unwind the DNA duplex and cleave both strands upon recognition of a target sequence by the guide RNA. The functional cassette synthesized in the rescue donor vector can then be inserted into the unwound DNA. The repaired genome will then express a desired sequence with or without tags.
Hamilton Robotics
NIMBUS PCR Workstation
According to Hamilton Robotics, this pipetting workstation is compact, affordable, and enables fast and flexible assay setup for endpoint, real-time, quantitative PCR or multiplex PCR. The workstation is PCR kit neutral and has an easy-to-use software wizard. It offers a variety of tip and labware types and can accommodate six master mixes and 192 PCRs. It can quickly configure different PCR assays and be used with basic Master Mix preparations and even restriction and ligation reaction setup. An additional feature allows for sample normalization either upstream for PCR setup or as a standalone function.
The INSTINCT PCR wizard uses a graphical interface to guide users through processing gene and sample combinations. The software traces all input components, completes setup information throughout the process, and saves all data for future use. The software allows protocols to be stored and includes validation steps to increase process safety. The INSTINCT wizard also has a flexible PCR plate-designing tool and displays real-time three-dimensional deck configurations.
More: www.hamiltonrobotics.com
Hamilton Storage Technologies
LabElite Benchtop Devices: Capper, DeCapper, and Reader
Three LabElite devices are available to process all common labware types. The all-in-one LabElite I.D. Capper enables decapping/recapping and high-speed barcode reading within one device without any additional user interaction. The I.D. Capper is designed to reduce sample contamination and assist with sample tracking during end-to-end automated liquid-handling processing.
The LabElite DeCapper is for decapping and recapping tubes in 48-cryovial or 96-microtube racks. It automatically moves the racks from portrait to landscape formats for processing. A secure mode provides sample security by decapping and recapping only one row at a time, minimizing the time a tube is open and eliminating the risk of cross-contamination. This feature also enables the rows to be paused for point-of-use pipetting between capping and recapping. The DeCapper can process all common tubes, including Matrix, FluidX, Greiner Bio-One, Micronic, Nunc, and Corning, with internal and external thread caps.
For manual workflows that need a high-speed two-dimensional (2D) barcode reader for tube racks, the LabElite I.D. Reader decodes multiple types of labware, including 2D barcoded tubes in all common 12-, 24-, 48-, 96-, and 384-tube racks including honeycomb-shaped racks, providing complete sample tracking during processing. The reader can identify racks that are labeled with a 2D barcode on the bottom. The technology uses high-speed decoding algorithms and parallel processing. A 96-tube rack runs in less than 3 s and a 384-tube rack in less than 5 s. Optional 1D barcode reading of the rack label is also available. The I.D. Reader supports all common tubes, including Matrix, FluidX, Greiner Bio-One, Micronic, Nunc, Corning, Matrical, WHEATON, ABgene, and REMP.
More: www.hamilton-storage.com
Micronic
2D Barcode Scanner for Frozen Samples
The Tracxer Code Reader RD235 CRYO now offers a high-end solution for scanning whole racks with 2D Data-Matrix or TraXis coded tubes. Featuring a CCD image sensor, the new reader provides high-resolution image quality for highly accurate 2D code reading. The Tracxer Code Reader RD235 CRYO features an antifrost system that minimizes condensation on the scanner plate, which makes tube codes from frozen samples readable.
As with all blue-white Tracxer Code Readers, the RD235 CRYO is easy to use, and its software allows users to scan a complete rack of 2D coded tubes in 7 s and a single tube in less than 2 s. Using the Tracxer Code Reader RD235 CRYO in conjunction with a Tracxer 1D Rack Barcode Reader BC235 accessory, users can read the 1D rack barcodes and 2D tube codes together.
The Tracxer Code Reader RD235 CRYO provides a complete tube code reading solution for users of 2D Data Matrix, TraXis, or competitive brand 2D-coded tubes to store samples. The reader is fully compatible with all ANSI/SLAS standard storage racks, which enables the reading of 12-, 24-, 48-, 96-, and even 384-well formats. Because of its compact size, the Micronic Tracxer Code Reader RD235 CRYO is easily integrated into robotic liquid-handling and storage systems.
More: www.micronic.com
Neoteryx
Mitra (RUO) Microsampling Device
In seconds, the Mitra microsampler collects 10 µL of fluid while eliminating the volumetric blood hematocrit bias typically associated with the dried blood spotting technique. Mitra is used for a wide range of biological sampling applications and is offered in two formats, including an automation-friendly option for high-throughput environments.
Based on volumetric absorptive microsampling technology, the Mitra (RUO) Microsampling Device is attuned to biological fluid collection needs. The design features facilitate high-throughput and serial sampling, fluid draws from live hosts or tubes, and the ability to collect precise microsamples with limited training and no specialized lab equipment. Once samples are collected, the Mitra microsampler is dried, stored, and transported under ambient conditions, eliminating the need for processing steps and transportation needs. To prepare samples for analysis, a simple and automatable extraction is performed with common solvents. The Mitra (RUO) Microsampling Device is available in two formats—96-well plate for high-throughput environments and as a four-pack clamshell. Sales, distribution, and technical support for the Mitra Microsampling Device are provided through Neoteryx’s distribution partner, Phenomenex.
More: www.phenomenex.com/mitra
Torrey Pines Scientific
EchoTherm Programmable HPLC Column Chiller/Heater
The EchoTherm Model CO50 has a temperature range from 4.0 °C to 100.0 °C readable and settable to 0.1 °C. The PID control software regulates temperatures to ±0.2 °C, even at ambient. The Peltier-based CO50 has a five-program memory of 10 steps per program and the ability to repeat any program from 1 to 99 times automatically. It is considered ideal for chiral and biomedical chromatography, in which below-ambient temperatures help preserve bioactivity. It can be used for stabilizing column temperatures from day to day at or near room temperatures for repeatable results. A stable temperature indicator lamp on the front panel of the unit lights when the target temperature is stable.
The CO50 holds columns up to 30-cm long by 1/4″ or 3/8″ diameter in mounting clips provided. Larger-diameter columns can be used by removing the column clips that hold the smaller columns. The CO50 features RS232 I/O port for controlling the unit by a chromatograph, for programming gradients, and for data collection. Also included are an injection counter, 30-d timer with user settable Auto-Off, and chamber drain for spills.
More: www.torreypinesscientific.com
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