Abstract
AMSBIO
ImmunoPure PBMCs
ImmunoPure peripheral blood mononuclear cells (PBMCs) are a variety of immune cells derived from the peripheral blood of normal healthy human adult donors collected at institutional review board–approved blood banks. Seven types are currently available and consist of a variety of immune cells derived from the peripheral blood of normal adults, thereby qualifying them for a diverse number of cell-based experiments. The lymphocyte and monocyte fractions are isolated from whole blood using apheresis, a technology in which the blood of a donor is passed through an apparatus that separates out these fractions and returns the remainder to the donor’s circulation. After depletion of granulocytes and red blood cells, these PBMCs are immediately cryopreserved using proprietary reagents to ensure high cell viability. All cell batches of ImmunoPure PBMCs are tested to be free from infectious agents and analyzed for cell viability, cell purity, cell count, and CD markers.
More: www.amsbio.com
Cultrex 3D Cell Proliferation Assays
AMSBIO now offers a range of 3D proliferation assays designed to make downstream applications of 3D cell culture easier. Cultrex Cell Proliferation Assays from AMSBIO were created in an effort to provide more physiologically relevant assessments when using cell models in the screening process for compounds that influence toxicity, cell survival, tumorigenicity, and new tumor formation. They offer a flexible, standardized, high-throughput format for quantitating the degree to which pharmacologic compounds influence toxicity or tumorigenicity in an in vivo–like environment. The Cell Proliferation Assay methodology has been adapted to multiple formats so that cell proliferation may be evaluated against different extracellular matrices. The assay is available for 3D growth in basement membrane extract (BME), laminin I, and collagen I formats.
PluriQ Serum Replacement
This optimized serum-free supplement is formulated as a direct replacement for fetal bovine serum for culturing healthy, undifferentiated human embryonic stems cell (hESCs) and induced pluripotent stem cells. PluriQ SR has been shown to maintain healthy cell morphology and normal karyotype in hESCs for at least 20 passages in culture. Every lot of PluriQ reagents is tested for quality, purity, and the effective maintenance of pluripotent stem cells over multiple passages to ensure health, morphology, and undifferentiation.
Bel-Art Products
Scienceware Roxy M Repeating Pipettor
According to Bel-Art Products, the ergonomic Roxy M and its range of universal fit pipettor tips provide reliable, repeat dispensing from 1 to 5000 µL. This lightweight pipettor (105 g) enables accurate and precise dispensing of a range of liquids, including high-viscosity or high-vapor-pressure liquids. Available sterile and nonsterile.
More: www.belart.com
Greiner Bio-One
CELLSTAR OneWell and FourWell Plates
Greiner Bio-One’s new OneWell Plate is a cell culture dish with a compartment that is considered well suited for cultivating and analyzing bacteria. Its format enables automated replication of bacteria colonies. The rectangular shape of the OneWell Plate and the fact that it can be stacked also allow efficient use of incubator space. For adherent cell culture, Greiner Bio-One has developed a model whose surface is hydrophilized using physical treatment.
With its four individual chambers, the FourWell Plate offers space for four slides to enable parallel experiments. Cell and tissue samples cultivated on these slides can be supplied with fresh medium quickly and easily and examined directly under a microscope. Semicircular cavities in the base of each well facilitate slide removal using tweezers. Spacers prevent the slides from slipping to the edge of the well and covering these cavities.
Both plates are made of transparent polystyrene and are sterile and free of detectable pyrogens, human DNA, and DNases/RNases. Their external dimensions comply with ANSI microplate standards, and the plates can be used with a variety of automated systems. A beveled edge on the lid and dish ensures the lid can be applied in only one direction. The plates can also be used as multipurpose containers for washing membranes (Southern, Northern, and Western blots).
CELLSTAR Products with Cell-Repellent Surface
CELLSTAR cell culture vessels are now available with cell-repellent surfaces, which have been specifically developed to effectively prevent the attachment of semiadherent and adherent cell lines. The cell-repellent surface is considered ideal for cultivating spheroids and embryoid bodies. The cell-repellent effect of the new surface is achieved through a stable chemical modification of the plastic. Unlike the conventional CELLSTAR suspension surface, the surface modification even prevents attachment of semiadherent and adherent cell lines such as macrophages.
The new CELLSTAR cell culture vessels with cell-repellent surface are free of detectable DNases, RNases, and human DNA. They contain no detectable endotoxins or cytotoxic substances, are sterile, have a shelf life of 2 y, and are offered for 96-well microplates with F or U bottom, 6-well multiwell plates, and the 100-mm cell culture dishes.
More: www.greinerbioone.com
INTEGRA Biosciences
Extended Length LONG GripTip Pipette Tip
The new, LONG GripTip pipette tip is available in nonsterile, sterile, filter, and Green Choice configurations. It is designed to work with single, 8-, 12-, and 16-channel VIAFLO electronic pipettes and VOYAGER 8- and 12-channel adjustable spacing electronic pipettes. The extended length design enables easy access into labware types such as 1.5 mL tubes and deep-well blocks. The extended length design also reduces PCR cross-contamination as the elongated tip prevents the pipette shaft from contacting the wall of the PCR tubes. Offering an extended volume range, 0.5 to 12.5 µL, the LONG GripTip allows users to repeat dispense 1 µL 12 times while still allowing for a 0.5 µL last dispense to improve accuracy and precision.
J.G. Finneran Associates
8- and 12-Channel Reservoir Trough Plates for Multichannel Pipettes
The troughs in these new plates hold liquid reagents in precise rows that align with 8-channel and 12-channel pipettes, allowing users to simultaneously aspirate or dispense fluids in all channels. They can help high-throughput laboratories improve speed and precision in performing serial dilutions or dispensing different reagents in up to 12 channels.
The 8- and 12-channel reservoir trough plates are constructed of high-impact plastics for durable, long-term performance. The reservoir design incorporates a v-shaped bottom with a residual volume of 200 µL, reducing dead volume and minimizing waste. For benchtop stability, the trough plates fit snugly on top of the Finneran 96-well multi-tier micro plate system. The 8-channel plate has a total capacity of 56 mL, with a 1 mL to 7 mL capacity per channel, and is constructed of high-impact polystyrene. The 12-channel plate is made from polyethylene terephthalate and has a 36 mL total capacity with a trough capacity of 1 mL to 3 mL.
More: www.jgfinneran.com
Micronic Europe
Octygen Screw Cap Sample Storage Tubes
Octygen externally threaded, 2D coded, 0.50 mL screw cap sample storage tubes are 25.6 mm tall and offer a 475 µL working volume because Octygen uses just 60% of the space typical 0.50 mL 96-well screw cap tubes use. In addition, 96 individual 0.5 mL Octygen tubes can be stored in an automation-compatible Micronic rack. Manufactured from high-purity polypropylene in a controlled environment compliant with ISO 14644 and medical manufacturing standard ISO 13485, each Octygen tube is uniquely identified with a laser-etched 2D Data-Matrix code that will never wear or fall off. Designed for both manual and automated low-temperature storage applications, the external thread design optimizes manual sample handling. A polypropylene cap/polypropylene tube seal in the rim of each Octygen tube and minimize diffusion of vapor during storage, even in dehumidified automation areas.
Laser-Etched Alphanumeric-Coded Sample Storage Tubes
Micronic’s new range of laser-etched alphanumeric-coded sample storage tubes (0.5 mL, 0.75 mL, 1.1 mL, and 1.4 mL) have a flat polypropylene bottom on which the alphanumeric code is encrypted with a laser. Manufactured from a single component, the codes cannot fade or peel off, even when subjected to repeated handling, exposure to different solvents, or multiple freeze-thaw cycles. Clarity will remain unchanged throughout their lifetime, providing secure sample traceability. Incorporating a smooth external flat surface, Micronic laser-etched alphanumeric tubes offer an easier-to-read and -identify alternative to traditional round-bottom alphanumeric tubes for automated applications.
Manufactured from high-quality polypropylene in a fully automated class 7 cleanroom environment ensures product consistency, near zero contaminants, and compliance with U.S. and European Pharmacopoeia tests. Micronic laser-etched alphanumeric tubes resist many organic solvents (DMSO, methanol, dichloromethane), may be autoclaved clean, and can be repeatedly freeze-thawed.
More: www.micronic.com
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