Abstract
American Type Culture Collection (ATCC)
Primary Adipose-Derived Mesenchymal Stem Cell Growth Solutions
ATCC now offers a complete solution to successfully grow normal human primary adipose-derived mesenchymal stem cells that includes stem cell differentiation, tissue engineering, cell therapy, regenerative medicine, and the creation of induced pluripotent stem cells. Each lot of ATCC normal human primary adipose-derived mesenchymal stem cells is tested for sample purity and cryopreserved in the second passage to ensure high viability and plating efficiency.
More: www.atcc.org
Primary Human Umbilical Vein Endothelial Cells
Human umbilical vein endothelial cells (HUVECs) are now available as part of ATCC’s Primary Cell Solutions for cardiovascular research. According to ATCC, these primary cells are derived from either single donors or a pooled population of unique donors. Pooled primary HUVECs minimize the lot-to-lot variation associated with single donor lots while yielding larger lot sizes for greater consistency in large-scale studies.
ATCC normal primary HUVECs are cryopreserved in the first or second passage and thoroughly tested for sample purity. Each lot of cells will grow for at least 15 population doublings while maintaining normal morphology and functionality. HUVECs from ATCC are demonstrated to be positive for von Willebrand factor and negative for smooth muscle alpha-actin. They are performance tested together with media, growth supplements, and reagents to guarantee reliability.
More: www.atcc.org/PCS2
AMS Biotechnology (AMSBIO)
Expression-Ready Lentiviral Particles
According to AMSBIO, this new range of ready-to-use lentiviral particles delivers genes in a highly reproducible and controllable manner for the expression of more than 250 human proteins, mouse proteins, enzymes, and fluorescent markers. Using protocols based on coexpressed red fluorescent protein by 2A peptide processing (SureTiter) or RSV promoter (Inducible system), AMSBIO lentiviral particles can enable real-time monitoring of protein expression. Suggested applications include gene delivery for expression or knockdown in hard-to-transfect cell types such as primary cells, stem cells, drug-arrested cells, and cells in suspension.
They also can create stable cell lines for long-term high constitutive expression or Tet-inducible expression applications in the presence of TetR (a repressor protein), and they can be used in vivo for generating transgenic animals and inducing pluripotent stem cells (iPSC). Custom lentiviral particles also can be generated.
More: www.amsbio.com/expression-ready-lentiviral-particles.aspx
Solu BL21 Competent Escherichia coli
This new BL21 host strain for soluble mammalian protein expression uses a directed evolution approach to develop a mutant strain of BL21(DE3) E. coli that can produce soluble protein in the majority of cases where expression in the parent BL21(DE3) yields no detectable soluble product. The Solu BL21 is compatible with all T7 promoter-based expression vectors.
More: www.amsbio.com/express-insoluble-proteins-in-soluble-form-SoluBL21.aspx
Oris Pro 384 Cell Migration Assay
The new Oris Pro 384 Cell Migration Assay is designed specifically for fully automated high-throughput screening (HTS) using high-content screening (HCS) and high-content imaging (HCI) systems. It uses a nontoxic biocompatible gel (BCG) to form a centrally located and temporary cell-free zone on cell culture surfaces in a 384-well format. After cell seeding, the BCG dissolves to reveal a detection zone into which the migration of cells can be observed either in real time or after further experimental processing.
Beckman Coulter
Krome Orange
According to Beckman Coulter, this violet-excited organic dye expands the range of available choices and dye sensitivity limits for violet lasers. Common gating markers can be transferred to this fluorochrome, freeing other channels for use with more esoteric markers and enabling more versatile 10-color flow cytometry applications. Krome Orange has excitation and emission maxima of 398 nm and 528 nm, respectively, and provides a brighter signal than Pacific Orange conjugates with equivalent spectral overlaps into adjacent channels. The new dye is at least as bright as V500 and can provide more than twice the population separation obtained with Pacific Orange dye conjugates, with little compensation compared to Pacific Blue dye. Krome Orange conjugates show optimal performance with violet laser excitation and a 550/40 bandpass filter (the standard FL10 channel on flow cytometry systems), whereas no excitation is detected using a 488-nm laser. The initial release of Krome Orange includes conjugates to human CD45 and CD4.
More: www.beckmancoulter.com
Cell Signaling Technology
PathScan Signaling Nodes Multiplex IF Kit #8999
This new multiplex assay allows users to simultaneously assess signaling through key pathway nodes using automated imaging or laser scanning high-content platforms or manual immunofluorescence microscopy. This kit allows the analysis of multiple pathway end points within a single sample and contains a cocktail of 3 primary antibodies, optimally formulated for use in conjunction with the provided Alexa Fluor detection cocktail. The kit provides reagents necessary to perform 100 assays (based on 100-µl volume).
More: www.cellsignal.com
Notch1 (D6F11) XP Rabbit mAb #4380
Notch1 is a transmembrane protein functioning in development and the determination of cell fate. During maturation, the notch molecule is cleaved by a furin-like convertase at its extracellular domain. Upon binding to a ligand such as Delta1 or upon extracellular calcium depletion, the carboxy-terminal notch1 fragment is released and further cleaved between Gly1743 and Va11744. The resulting activated cytosolic fragment translocates to the nucleus where it activates transcription.
Porvair Sciences
MicroBurst Clear Sealing Film
With its adhesive encapsulated in millions of tiny spheroids, MicroBurst sealing film, when first handled, is not sticky and can be quickly and easily moved into position. Pressing down hard when the film is in the correct place bursts and activates the adhesive microcapsules. According to Porvair, the film has excellent optical properties and produces a very firm seal that quickly strengths to form a near-permanent seal on your plates. MicroBurst film can be pierced by pipette tips or robot liquid handlers and is temperature stable.
More: www.porvair-sciences.com
24-Well Microplates for Large-Scale, High-Yield Growth of Yeast
Produced under class 10,000 clean room conditions from virgin polypropylene, the new 24-well plate has a working volume of 10 ml/well and contains no contaminants that may leach out and affect yeast growth. The wells of the high-volume plate are rectangular in shape with a “v” bottom to facilitate easy yeast concentration and recovery.
More: www.porvair-sciences.com
Low-Profile 96-Well Microplate
Designed to minimize space required for compound storage, this new low-profile 96-well plate achieves a storage volume of 1.1 ml/well in a height of just 27 mm. A new matching antievaporation cap mat, manufactured from thermoplastic elastomer, is also available to ensure long-term integrity of stored samples.
More: www.porvair-sciences.com
Thermo Fisher Scientific
Nunclon Vita Stem Cell Culture Surface
According to Thermo, this new, energy-treated polystyrene surface enables direct attachment, colony formation, and growth of human embryonic stem cells (ESCs) and induced pluripotent stem cells (IPSs), without the need for feeder cells or matrix coatings. In media supplemented with a Rho-associated kinase (ROCK) inhibitor, human ESCs can be cultured on the Nunclon Vita Surface for at least 10 passages without any loss of pluripotency or karyotype changes. By eliminating matrix coatings and feeder cells, the Nunclon Vita surface reduces culture variability and is less labor intensive. The Nunclon Vita surface treatment is considered more adaptable for scalable cell expansion as cells grow directly on the polystyrene surface. In addition, the new surface works in synergy with a ROCK inhibitor for expansion of human ESC, IPS, and human embryonic kidney cells to enable enzymatic and mechanical passage protocols.
More: www.thermoscientific.com/nunclonvita
