Potential risk genotypes associated with symptoms of pesticide poisoning based on polymorphisms in the XRCC1 ( rs25487 and rs1799782 ),SOD2 ( rs4880 ),PON1 ( rs662 ) and GSTP1 ( rs1695 ) genes,in farmers in the Cienega region
Restricted accessResearch articleFirst published online May, 2026
Potential risk genotypes associated with symptoms of pesticide poisoning based on polymorphisms in the XRCC1 ( rs25487 and rs1799782 ),SOD2 ( rs4880 ),PON1 ( rs662 ) and GSTP1 ( rs1695 ) genes,in farmers in the Cienega region
The variants in the genes involved in the defense mechanisms against pesticide poisoning could be the cause to develop symptoms of poisoning with these substances. In this work, we analyzed polymorphisms in genes that defend against pesticide poisoning in 112 farmers from the Cienega region of Jalisco. We genotyped the variants rs25487 and rs1799782 of the XRCC1 gene, rs28360135 of the XRCC4 gene, rs4880 of the SOD2 gene and rs662 and rs1695 of the PON1 and GSTP1 genes, respectively. For rs25487 (AA vs AG; p = 0.045), significance was found with heart palpitations, rs1799782 with salivation (CC vs CT; p = 0.038), rs4880 with sweating (TT+TC vs CC; p = 0.026; TC vs CC; p = 0.034) and rs662 with cough (AA vs AG+GG; p = 0.001; AA vs AG; p = 0.006; AA vs GG; p = 0.027) and salivation (AG vs GG; p = 0.037). Furthermore, the variant rs1799782 found significance with carbamates (p = 0.049) and pyrethroids (p = 0.015), as well as the TT versus TC+CC (p = 0.043) and TT versus TC (p = 0.034) genotypes of rs4880 with carbamates. Finally, AA+AG versus GG (p = 0.039) and AG versus GG (p = 0.025) genotypes of the rs1695 polymorphism were also significant with carbamates. Except for the XRCC4 rs28360135 and GSTP1 rs1695 variants, the rest of the polymorphisms showed significance with symptoms of acute poisoning. The presence of XRCC1 (rs1799782), SOD2 (rs4880) and GSTP1 (rs1695) variants may be risk factors for carbamate and pyrethroid poisoning.
AbubakarYTijjaniHEgbunaC, et al. Pesticides, history, and classification. In: EgbunaCSawickaB (eds) Natural remedies for pest, disease and weed control. Academic Press, 2020, pp.29–42.
2.
SharmaAShuklaAAttriK, et al. Global trends in pesticides: a looming threat and viable alternatives. Ecotoxicol Environ Saf2020; 201: 110812.
3.
Ceja GalvezHRSalazar FloresJTorres SanchezED, et al. Genetic profile for the detection of susceptibility to poisoning by exposure to pesticides. Ann Agric Environ Med2021; 28(2): 208–213.
4.
PingarilhoMOliveiraNGMartinsC, et al. Genetic polymorphisms in detoxification and DNA repair genes and susceptibility to glycidamide-induced DNA damage. J Toxicol Environ Health A2012; 75(13–15): 920–933.
5.
Pérez-RamírezCCañadas-GarreMMolinaMÁ, et al. Contribution of genetic factors to platinum-based chemotherapy sensitivity and prognosis of non-small cell lung cancer. Mutat Res Rev Mutat Res2017; 771: 32–58.
6.
SaadAMAbdel-MegiedAESElbazRA, et al. Genetic variants of APEX1 p.Asp148Glu and XRCC1 p.Gln399Arg with the susceptibility of hepatocellular carcinoma. J Med Virol2021; 93(11): 6278–6291.
7.
ChangTTSunMFChangKW, et al. Screening from the world’s largest TCM database for inhibiting DNA repair protein XRCC4. Mol Simul2011; 37 (6): 503–509.
8.
KahlVFSda SilvaFRAlvesJDS, et al. Role of PON1, SOD2, OGG1, XRCC1, and XRCC4 polymorphisms on modulation of DNA damage in workers occupationally exposed to pesticides. Ecotoxicol Environ Saf2018; 159: 164–171.
9.
FlynnJMMelovS.SOD2 in mitochondrial dysfunction and neurodegeneration. Free Radic Biol Med2013; 62: 4–12.
10.
FernandesRCHasanMGuptaH, et al. Host genetic variations in glutathione-S-transferases, superoxide dismutases and catalase genes influence susceptibility to malaria infection in an Indian population. Mol Genet Genomics2015; 290(3): 1155–1168.
11.
AlvesAALaurinhoKFrancoFC, et al. The incidence of the XRCC1 rs25487 and PON1 rs662 polymorphisms in a population from Central Brazil: patterns in an area with a high level of agricultural activity. Biochem Genet2023; 61(5): 1675–1703.
12.
LvFMaYZhangY, et al. Relationship between GSTP1 rs1695 gene polymorphism and myelosuppression induced by platinum-based drugs: a meta-analysis. Int J Biol Markers2018; 33(4): 364–371.
13.
OrtízIAvila-ChávezMTorresL.Plaguicidas en México: usos, riesgos y marco regulatorio. Rev Latinoam Biotecnol Ambient Algal2014; 5(3): 26–46.
14.
Salazar-FloresJPacheco-MoisésFPOrtizGG, et al. Occupational exposure to organophosphorus and carbamates in farmers in La Cienega, Jalisco, Mexico: oxidative stress and membrane fluidity markers. J Occup Med Toxicol2020; 15: 32.
15.
García-HernándezJLeyva-MoralesJBBastidas-BastidasPJ, et al. A comparison of pesticide residues in soils from two highly technified agricultural valleys in northwestern Mexico. J Environ Sci Health B2021; 56(6): 548–565.
16.
Silva-MaderaRJSalazar-FloresJPeregrina-LucanoAA, et al. Pesticide contamination in drinking and surface water in the Cienega, Jalisco, Mexico. Water Air Soil Pollut2021; 232(2): 1–13.
17.
ExcoffierLLischerHE.Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour2010; 10(3): 564–567.
18.
MaWQHanXQWangX, et al. Associations between XRCC1 gene polymorphisms and coronary artery disease: a meta-analysis. PLoS One2016; 11(11): e0166961.
19.
BazoAPSalvadoriD JrSalvadoriRA, et al. DNA repair gene polymorphism is associated with the genetic basis of atherosclerotic coronary artery disease. Cardiovasc Pathol2011; 20(1): e9–e15.
20.
GoncharovaIAMakeevaOAGolubenkoMV, et al. Genes for fibrogenesis in the determination of susceptibility to myocardial infarction. Mol Biol2016; 50(1): 94–105 (Russian).
21.
HoseiniSMBijanzadehMSeyedianSM.A DNA repair pathway polymorphism (rs25487) and angiographically proven coronary artery patients in a population of Southern Iran. Cardiovasc Hematol Agents Med Chem2021; 19(1): 76–82.
GeorgiadisNTsarouhasKTsitsimpikouC, et al. Pesticides and cardiotoxicity. Where do we stand?Toxicol Appl Pharmacol2018; 353: 1–14.
25.
PeritoreAFFrancoGAMolinariF, et al. Effect of pesticide vinclozolin toxicity exposure on cardiac oxidative stress and myocardial damage. Toxics2023; 11(6): 473.
26.
Macías-GómezNMPeralta-LealVMeza-EspinozaJP, et al. Polymorphisms of the XRCC1 gene and breast cancer risk in the Mexican population. Fam Cancer2015; 14(3): 349–354.
27.
Meza-EspinozaJPPeralta-LealVDurán-GonzálezJ, et al. XRCC1 R194W and R399Q polymorphisms and colorectal cancer risk in a northeastern Mexican population. Genet Res2023; 2023: 5565646.
28.
Przybylowska-SygutKStanczykMKusinskaR, et al. Association of the Arg194Trp and the Arg399Gln polymorphisms of the XRCC1 gene with risk occurrence and the response to adjuvant therapy among Polish women with breast cancer. Clin Breast Cancer2013; 13(1): 61–68.
29.
JalaliCGhaderiBAminiS, et al. Association of XRCC1 Trp194 allele with risk of breast cancer, and Ki67 protein status in breast tumor tissues. Saudi Med J2016; 37(6): 624–630.
30.
LiQMaRZhangM.XRCC1 rs1799782 (C194T) polymorphism correlated with tumor metastasis and molecular subtypes in breast cancer. Onco Targets Ther2018; 11: 8435–8444.
31.
PengMZhouXDingX, et al. Association of XRCC1 Arg399Gln and Arg194Trp polymorphisms with susceptibility to multiple autoimmune diseases: a meta-analysis. Rheumatol Int; 37(3): 435–444.
32.
ZhangYWangYWuJ, et al. XRCC1 Arg194Trp polymorphism is associated with oral cancer risk: evidence from a meta-analysis. Tumour Biol2013; 34(4): 2321–2327.
33.
MozaffariHRRostamniaMSharifiR, et al. A PRISMA-compliant meta-analysis on association between X-ray repair cross complementing (XRCC1, XRCC2, and XRCC3) polymorphisms and oral cancer susceptibility. Gene2021; 781: 145524.
34.
HoTLiGLuJ, et al. X-ray repair cross-complementing group 1 (XRCC1) single-nucleotide polymorphisms and the risk of salivary gland carcinomas. Cancer2007; 110(2): 318–325.
35.
MosaediMHSeifiSMohahammadpourA, et al. Association between XRCC1 (rs1799782) gene polymorphism and oral squamous cell carcinoma. J Mazandaran Univ Med Sci2022; 31(204): 40–48 (Persian).
36.
MrouehRTanskanenTHaapaniemiA, et al. Familial cancer risk in family members and spouses of patients with early-onset head and neck cancer. Head Neck2020; 42(9): 2524–2532.
37.
WongRHChangSYHoSW, et al. Polymorphisms in metabolic GSTP1 and DNA-repair XRCC1 genes with an increased risk of DNA damage in pesticide-exposed fruit growers. Mutat Res2008; 654(2): 168–175.
38.
KaurKKaurR.Impact of single nucleotide polymorphisms in the OGG1 and XRCC1 genes on modulation of DNA damage in pesticide-exposed agricultural workers in Punjab, North-West India. Biomarkers2020; 25(6): 498–505.
39.
GarshinAAltynovaNDjangalinaE, et al. Individual risk assessment for population living on the territories long-term polluted by organochlorine pesticides. Toxics2023; 11(6): 482.
40.
ZhangWSuYYueG, et alCorrelations of SDF-1α and XRCC1 gene polymorphisms with the risk of renal cancer development and bioinformatics studies of SDF-1α and XRCC1 and the prognosis of renal cancer. Sci Rep2024; 14(1): 3367.
41.
Ascencio-Montiel IdeJParraEJValladares-SalgadoA, et al. SOD2 gene Val16Ala polymorphism is associated with macroalbuminuria in Mexican type 2 diabetes patients: a comparative study and meta-analysis. BMC Med Genet2013; 14: 110.
42.
Gallegos-ArreolaMPRamírez-PatiñoRSánchez-LópezJY, et al. SOD2 gene variants (rs4880 and rs5746136) and their association with breast cancer risk. Curr Issues Mol Biol2022; 44(11): 5221–5233.
43.
Jiménez-OrtegaRFAparicio-BautistaDIBecerra-CerveraA, et al. Association study between antioxidant nutrient intake and low bone mineral density with oxidative stress-single nucleotide variants: GPX1 (rs1050450 and rs17650792), SOD2 (rs4880) and CAT (rs769217) in Mexican women. Antioxidants2023; 12(12): 2089.
44.
KangSYLeeKGLeeW, et al. Polymorphisms in the DNA repair gene XRCC1 associated with basal cell carcinoma and squamous cell carcinoma of the skin in a Korean population. Cancer Sci2007; 98(5): 716–720.
45.
WangVChenSYChuangTC, et al. Val-9Ala and Ile+58Thr polymorphism of MnSOD in Parkinson’s disease. Clin Biochem2010; 43(12): 979–982.
46.
HirtPACastilloDEYosipovitchG, et al. Skin changes in the obese patient. J Am Acad Dermatol2019; 81(5): 1037–1057.
47.
YosipovitchGDeVoreADawnA.Obesity and the skin: skin physiology and skin manifestations of obesity. J Am Acad Dermatol2007; 56(6): 901–916; quiz 917–920.
48.
LewandowskiŁUrbanowiczIKepinskaM, et al. Concentration/activity of superoxide dismutase isozymes and the pro-/antioxidative status, in context of type 2 diabetes and selected single nucleotide polymorphisms (genes: INS, SOD1, SOD2, SOD3)—preliminary findings. Biomed Pharmacother2021; 137: 111396.
49.
YuJQiuJZhangZ, et al. Redox biology in adipose tissue physiology and obesity. Adv Biol2023; 7(9): e2200234.
50.
GustiAMTQustiSYAlshammariEM, et al. Antioxidants-related superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (GST), and nitric oxide synthase (NOS) gene variants analysis in an obese population: a preliminary case–control study. Antioxidants2021; 10(4): 595.
51.
ValeALottiM.Organophosphorus and carbamate insecticide poisoning. Handb Clin Neurol2015; 131: 149–168.
52.
DebnathMKhanMS.Health concerns of pesticides. In: KhanMRahmanM (eds.) Pesticide residue in foods: sources, management, and control. Springer, 2017, pp.103–118.
53.
HeKZhangSPangJ, et al. Genomic profiling reveals novel predictive biomarkers for chemo-radiotherapy efficacy and thoracic toxicity in non-small-cell lung cancer. Front Oncol2022; 12: 928605.
54.
MansourSAMossaATHeikalTM.Effects of methomyl on lipid peroxidation and antioxidant enzymes in rat erythrocytes: in vitro studies. Toxicol Ind Health2009; 25(8): 557–563.
55.
Alegría-TorresJAGarcía-DomínguezMLCruzM, et al. Q192R polymorphism of paraoxonase 1 gene associated with insulin resistance in Mexican children. Arch Med Res2015; 46(1): 78–83.
56.
Hernández-CollazoAAPérez-MéndezOLópez-OlmosV, et al. Association between rs662 (A > G) and rs854560 (A > T) polymorphisms in PON1 gene and the susceptibility for psoriasis in mestizo population of Western Mexico. Mol Biol Rep2021; 48(1): 183–194.
57.
SeoTPahwaPMcDuffieHH, et al. Interactive effect of paraoxonase-1 Q192R polymorphism and smoking history on the lung function decline in grain workers. Ann Epidemiol2008; 18(4): 330–334.
58.
RodrigoLHernándezAFLópez-CaballeroJJ, et al. Immunohistochemical evidence for the expression and induction of paraoxonase in rat liver, kidney, lung and brain tissue. Implications for its physiological role. Chem Biol Interact2001; 137(2): 123–137.
59.
EomSYYimDHLeeCH, et al. Interactions between paraoxonase 1 genetic polymorphisms and smoking and their effects on oxidative stress and lung cancer risk in a Korean population. PLoS One2015; 10(3): e0119100.
60.
CostaLGRichterRJLiWF, et al. Paraoxonase (PON 1) as a biomarker of susceptibility for organophosphate toxicity. Biomarkers2003; 8(1): 1–12.
61.
EomSYKimYSLeeCJ, et al. Effects of intronic and exonic polymorphisms of paraoxonase 1 (PON1) gene on serum PON1 activity in a Korean population. J Korean Med Sci2011; 26(6): 720–725.
62.
LeeCHLeeKYChoeKH, et al. Effects of oxidative DNA damage induced by polycyclic aromatic hydrocarbons and genetic polymorphism of the paraoxonase-1 (PON1) gene on lung cancer. J Prev Med Public Health2005; 38(3): 345–350 (Korean).
63.
Aksoy-SagirliPCakmakogluBIsbirT, et al. Paraoxonase-1 192/55 polymorphisms and the risk of lung cancer in a Turkish population. Anticancer Res2011; 31(6): 2225–2229.
64.
WangHLiLDingL, et al. Association of genetic polymorphisms in the paraoxonase 1 gene with the risk and prognosis of non-small cell lung cancer in Chinese Han population. J Investig Med2012; 60(3): 592–597.
65.
ZayedAAAhmedAIKhattabAM, et al. Paraoxonase 1 and cytochrome P450 polymorphisms in susceptibility to acute organophosphorus poisoning in Egyptians. Neurotoxicology2015; 51: 20–26.
66.
PearsonJNPatelM.The role of oxidative stress in organophosphate and nerve agent toxicity. Ann N Y Acad Sci2016; 1378(1): 17–24.
67.
Taler-VerčičAGoličnikMBavecA.The structure and function of paraoxonase-1 and its comparison to paraoxonase-2 and -3. Molecules2020; 25(24): 5980.
68.
LiuYJHuangPLChangYF, et al. GSTP1 genetic polymorphism is associated with a higher risk of DNA damage in pesticide-exposed fruit growers. Cancer Epidemiol Biomarkers Prev2006; 15(4): 659–666.
69.
SinghSKumarVSinghP, et al. Genetic polymorphisms of GSTM1, GSTT1 and GSTP1 and susceptibility to DNA damage in workers occupationally exposed to organophosphate pesticides. Mutat Res2011; 725(1–2): 36–42.
70.
Saad-HusseinANoshyMTahaM, et al. GSTP1 and XRCC1 polymorphisms and DNA damage in agricultural workers exposed to pesticides. Mutat Res Genet Toxicol Environ Mutagen2017; 819: 20–25.
