Morinda officinalis polysaccharide promotes the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells via microRNA-210-3p/scavenger receptor class A member 3

Abstract

Graphical abstract

MOP promotes osteogenic differentiation and cell viability and inhibits adipogenic differentiation and apoptosis in hBMSCs. In addition, MOP increased BMD and inhibited TRACP 5b and NTx levels in OVX rats.

Morinda officinalis polysaccharide (MOP) is the bioactive ingredient extracted from the root of Morinda officinalis, and Morinda officinalis is applied to treat osteoporosis (OP). The purpose of this study was to determine the role of MOP on human bone marrow mesenchymal stem cells (hBMSCs) and the underlying mechanism. HBMSCs were isolated from bone marrow samples of patients with OP and treated with MOP. Quantitative real-time polymerase chain reaction was adopted to quantify the expression of microRNA-210-3p (miR-210-3p) and scavenger receptor class A member 3 (SCARA3) mRNA. Cell Counting Kit-8 assay was employed to detect cell viability; Terminal-deoxynucleotidyl Transferase Mediated Nick End Labeling assay and flow cytometry were adopted to detect apoptosis; Alkaline Phosphatase (ALP) activity assay kit was applied to detect ALP activity; Western blot was executed to quantify the expression levels of SCARA3, osteogenic and adipogenic differentiation markers. Ovariectomized rats were treated with MOP. Bone mineral density (BMD), serum tartrate-resistant acid phosphatase 5b (TRACP 5b), and N-telopeptide of type I collagen (NTx) levels were assessed by BMD detector and Enzyme-linked immunosorbent assay kits. It was revealed that MOP could promote hBMSCs’ viability and osteogenic differentiation and inhibit apoptosis and adipogenic differentiation. MOP could also upregulate SCARA3 expression through repressing miR-210-3p expression. Treatment with MOP increased the BMD and decreased the TRACP 5b and NTx levels in ovariectomized rats. MOP may boost the osteogenic differentiation and inhibit adipogenic differentiation of hBMSCs by miR-210-3p/SCARA3 axis.

Keywords

Morinda officinalis polysaccharide human bone marrow-derived mesenchymal stem cells osteogenic differentiation

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References

Armas

Recker

. Pathophysiology of osteoporosis: new mechanistic insights. Endocrinol Metab Clin North Am 2012; 41(3): 475–486.

Office of the Surgeon General (US). Bone health and osteoporosis: a report of the surgeon general. Rockville, MD: Office of the Surgeon General (US), 2004.

Awasthi

Mani

Singh

, et al. The underlying pathophysiology and therapeutic approaches for osteoporosis. Med Res Rev 2018; 38(6): 2024–2057.

Yan

Xie

, et al. Combined treatment with cinnamaldehyde and PTH enhances the therapeutic effect on glucocorticoid-induced osteoporosis through inhibiting osteoclastogenesis and promoting osteoblastogenesis. Biochem Biophys Res Commun 2018; 505(3): 945–950.

Shea

Sorauf

Polson

, et al. One-dollar medications: evaluating the true cost. Adv Transl Med 2022; 1(1): 17–32.

Lee

Long

. Notch signaling suppresses glucose metabolism in mesenchymal progenitors to restrict osteoblast differentiation. J Clin Invest 2018; 128(12): 5573–5586.

Baker

Boyette

Tuan

. Characterization of bone marrow-derived mesenchymal stem cells in aging. Bone 2015; 70: 37–47.

Rastegar

Shenaq

Huang

, et al. Mesenchymal stem cells: molecular characteristics and clinical applications. World J Stem Cells 2010; 2(4): 67–80.

Wang

Deng

Chen

, et al. LncRNA MIAT can regulate the proliferation, apoptosis, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells by targeting miR-150-5p. Bioengineered 2022; 13(3): 6343–6352.

10.

Qiu

Zhao

, et al. Ataxia-televangelist mutated (ATM)/ATR serine/threonine kinase (ATR)-mediated RAD51 recombinase (RAD51) promotes osteogenic differentiation and inhibits osteoclastogenesis in osteoporosis. Bioengineered 2022; 13(2): 4201–4211.

11.

Zhang

Xin

, et al. Morinda officinalis How. —A comprehensive review of traditional uses, phytochemistry and pharmacology. J Ethnopharmacol 2018; 213: 230–255.

12.

Zheng

Qin

, et al. Antiosteoporotic activity of anthraquinones from Morinda officinalis on osteoblasts and osteoclasts. Molecules 2009; 14(1): 573–583.

13.

Zhang

Yang

, et al. Monotropein isolated from the roots of Morinda officinalis increases osteoblastic bone formation and prevents bone loss in ovariectomized mice. Fitoterapia 2016; 110: 166–172.

14.

Yan

Huang

Shen

, et al. Identification and characterization of a polysaccharide from the roots of Morinda officinalis, as an inducer of bone formation by up-regulation of target gene expression. Int J Biol Macromol 2019; 133: 446–456.

15.

Zheng

, et al. CircRNA_0001795 sponges miRNA-339-5p to regulate yes-associated protein 1 expression and attenuate osteoporosis progression. Bioengineered 2022; 13(2): 2803–2815.

16.

Wang

Feng

, et al. β-Patchoulene represses hypoxia-induced proliferation and epithelial-mesenchymal transition of liver cancer cells. Bioengineered 2022; 13(5): 11907–11922.

17.

Noack

Hempel

Preissler

, et al. Prostaglandin E2 impairs osteogenic and facilitates adipogenic differentiation of human bone marrow stromal cells. Prostaglandins Leukot Essent Fatty Acids 2015; 94: 91–98.

18.

Zhu

Peng

, et al. Morinda officinalis oligosaccharides ameliorate depressive-like behaviors in poststroke rats through upregulating GLUT3 to improve synaptic activity. FASEB J 2020; 34(10): 13376–13395.

19.

Huang

Qiu

Zhang

, et al. Nitroglycerin enhances proliferation and osteoblastic differentiation in human mesenchymal stem cells via nitric oxide pathway. Acta Pharmacol Sin 2008; 29(5): 580–586.

20.

Zhang

, et al. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway. Acta Pharmacol Sin 2012; 33(4): 479–489.

21.

Xin

Cai

. The role of resveratrol in bone marrow-derived mesenchymal stem cells from patients with osteoporosis. J Cell Biochem 2019; 120(10): 16634–16642.

22.

Raggatt

Partridge

. Cellular and molecular mechanisms of bone remodeling. J Biol Chem 2010; 285(33): 25103–25108.

23.

Zhang

Liu

, et al. Vitamin K2 stimulates MC3T3E1 osteoblast differentiation and mineralization through autophagy induction. Mol Med Rep 2019; 19(5): 3676–3684.

24.

Liu

Lee

. Transcriptional regulatory cascades in Runx2-dependent bone development. Tissue Eng Part B Rev 2013; 19(3): 254–263.

25.

Moseti

Regassa

Kim

. Molecular regulation of adipogenesis and potential anti-adipogenic bioactive molecules. Int J Mol Sci 2016; 17(1): 124.

26.

Nenonen

Cheng

Ivaska

, et al. Serum TRACP 5b is a useful marker for monitoring alendronate treatment: comparison with other markers of bone turnover. J Bone Miner Res 2005; 20(10): 1804–1812.

27.

Das

Khodarkovskaya

, et al. Non-coding RNAs and their bioengineering applications for neurological diseases. Bioengineered 2021; 12(2): 11675–11698.

28.

Yang

Yujiao

Fang

, et al. The roles of miRNA, lncRNA and circRNA in the development of osteoporosis. Biol Res 2020; 53(1): 40.

29.

Ren

Yang

Dai

, et al. Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-kappaB signaling pathway. Mol Cancer 2017; 16(1): 117.

30.

Wang

, et al. Downregulation of RGMA by HIF-1A/miR-210-3p axis promotes cell proliferation in oral squamous cell carcinoma. Biomed Pharmacother 2019; 112: 108608.

31.

Chen

Hou

Cai

. MiR-210-3p Enhances cardiomyocyte apoptosis and mitochondrial dysfunction by targeting the NDUFA4 gene in sepsis-induced myocardial dysfunction. Int Heart J 2021; 62(3): 636–646.

32.

Chen

, et al. Identification of differentially expressed microRNAs in the bone marrow of osteoporosis patients. Am J Transl Res 2019; 11(5): 2940–2954.

33.

Peng

Zhu

, et al. MiR-210-3p inhibits osteogenic differentiation and promotes adipogenic differentiation correlated with Wnt signaling in ERalpha-deficient rBMSCs. J Cell Physiol 2019; 234(12): 23475–23484.

34.

Zhu

Yuan

, et al. The function and mechanism of the miR-210-3p/KRAS axis in bone marrow-derived mesenchymal stem cell from patients with osteoporosis. J Tissue Eng Regen Med 2021; 15(8): 699–711.

35.

Chen

Tao

, et al. Regulatory network of miRNA on its target: coordination between transcriptional and post-transcriptional regulation of gene expression. Cell Mol Life Sci 2019; 76(3): 441–451.

36.

Han

H J

Tokino

Nakamura

. CSR, a scavenger receptor-like protein with a protective role against cellular damage caused by UV irradiation and oxidative stress. Hum Mol Genet 1998; 7(6): 1039–1046.

37.

Brown

Schibler

Fitzgerald

, et al. Scavenger receptor class A member 3 (SCARA3) in disease progression and therapy resistance in multiple myeloma. Leuk Res 2013; 37(8): 963–969.

38.

Tian

Zhou

, et al. Scavenger receptor class a, member 3 is associated with severity of hand, foot, and mouth disease in a case-control study. Medicine (Baltimore) 2019; 98(40): e17471.

39.

Chen

Xie

L Q

, et al. Scara3 regulates bone marrow mesenchymal stem cell fate switch between osteoblasts and adipocytes by promoting Foxo1. Cell Prolif 2021; 54(8): e13095.