A method is presented using a new reagent containing propylgallate for the quantitative determina tion of lupus anticoagulant. The amount of an optimized phospholipid standard required by the clotting reaction was found to be 32-50 μg/ml at a 95% confidence level, with a mean of 41 μg/ml. This method eliminates the ef fect of heparin therapy, coumadin therapy, factor-VIII inhibitor, factor-IX inhibitor, and single-factor deficien cies that afflict presently used lupus anticoagulant screen ing and confirmatory procedures. Using this method, it should be possible to detect lupus anticoagulant in pa tients at a much lower level and follow the effect of ther apy on lupus anticoagulant.
Mintz G., Acevedo-Vazquez E., Gutierrez-Espinosa G., et al. Renal vein thrombosis and inferior vena cava thrombosis in systemic erythematosis. Arthritis Rheum1984;27:539.
2.
Padmakumar K. , Singh RR, Rai R., et al. Lupus anticoagulants in systemic erythematosis: prevalence and clinical associations. Ann Rheum Dis1991 ;498:986.
3.
Averbuch M., Koifman B., Levo Y.Lupus anticoagulant, thrombosis and thrombocytopenia in systemic lupus erythematosis . Am J Med Sci1987;293:1.
Hughes Grv.Thrombosis, abortion, cerebral disease and the lupus anticoagulant . BMJ1983;287:1088.
6.
Hughes Grv.An immune mechanism in thrombosis. Q J Med1988;258:753.
7.
Barna LK, Triplett DAA report on the first international workshop for lupus anticoagulant identification . Clin Exp Rheumatol1991;9:557.
8.
Saxena R., Saraya AK, Kotte VK, et al. Evaluation of four coagulation tests to detect plasma lupus anticoagulants. Am J Clin Pathol1991;96:755.
9.
Hemostasis Committee of the Societé Française de Biologie Clinique , Angeles-Cano E ed. Laboratory heterogeneity of the lupus anticoagulant: a multi-centre study using different clotting assays on a panel of 78 samples. Thromb Res1992;66:349.
10.
Triplett DACoagulation assays for lupus anticoagulant: a review and critique of current methodology. Stroke.1992;23 (suppl 1):1-11.
11.
Marshall LRDetection of lupus anticoagulant: an Australian perspective. Pathology1992;25:360.
12.
Messmore H. , Fabbrini N., Hoppensteadt D., et al. Lupus anticoagulant assays. Semin Thromb Hemost1994;20:79.
13.
Feng C., Sophia SF, Tsang Ssf, et al. Evaluation of laboratory tests for lupus anticoagulant in a group of Chinese patients. Pathology1994;26:40.
14.
Lupus Working Party on behalf of the BCSH Hemostasis and Thrombosis Task Force: Guidelines on testing for the lupus anticoagulant. J Clin Pathol44:885.
15.
Exner T., Triplett DA, Taberner D., Machin SJGuidelines for testing and revised criteria for lupus anticoagulants, SSC subcommittee for the Standardization of Lupus Anticoagulants. Thromb Haemost1991 ;65:320.
