Polymorphonuclear Leukocyte Integrinsin Deep Venous Thrombosis

Abstract

The polymorphonuclear leukocytes (PMN) have a role in the pathophysiology of deep venous thrombosis (DVT). We examined the phenotypical expression of PMN beta₂-integrins (CD l l a, CDl l b, CD 11c) in a group of 19 subjects with leg DVT. PMN cells were incubated with fluorescent monoclonal antibodies against CD11a, CD11b, CD11c, and the evaluation was made by flow cytofluorimetry. The same integrins were determined after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and N-formylmethionyl-leucyl-phenylalanine (fMLP). In DVT subjects, at baseline, the phenotypical expression of CD11b was decreased and that of CD11c increased when compared with normal controls. In normal subjects PMN activation with PMA and fMLP led to a constant increase of all PMN adhesion molecules, while in DVT subjects the CDl l a did not show any change. These data might have therapeutical ap plications, especially with the aim of preventing post-thrombotic deterioration of vein function.

Keywords

Deep venous thrombosis Polymorphonuclear leukocyte integrins Polymorphonuclear leukocyte activation

References

Stewart GJ . Neutrophils and deep venous thrombosis. Haemostasis 1993;23:127.

Whiston RJ , Hallett MB , Davies EV , et al. Inappropriate neutrophil activation in venous disease. Br J Surg 1994;81:695.

Downing LJ , Strieter RM , Kadell AM , et al. Neutrophils are the initial cell type identified in deep venous thrombosis induced vein wall inflammation. ASAIO J 1996;42:M677.

Wakefield TW , Strieter RM , Prince MR , et al. Pathogenesis of venous thrombosis: A new insight. Cardiovasc Surg 1997;5:6.

Reiter M , Bucek RA , Koca N , et al. Deep vein thrombosis and systemic inflammatory response: A pilot trial. Wien Klin Wochenschr 2003;115:111.

Wakefield TW , Strieter RM , Wilke CA , et al. Venous thrombosis-associated inflammation and attenuation with neutralizing antibodies to cytokines and adhesion molecules. Arterioscler Thromb Vasc Biol 1995;15:258.

Wakefield TW , Strieter RM , Downing LJ , et al. P-selectin and TNF inhibition reduce venous thrombosis inflammation. J Surg Res 1996;64:26.

Myers DD Jr , Henke PK , Wrobleski SK , et al. P-selectin inhibition enhances thrombus resolution and decreases vein wall fibrosis in a rat model. J Vasc Surg 2002;36:928.

Thanaporn P , Myers DD , Wrobleski SK , et al. P-selectin inhibition decreases post-thrombotic vein wall fibrosis in a rat model. Surgery 2003;134:365.

10.

Hogevold HE , Lyberg T , Kahler H , et al. Expression of beta-2-integrins and L-selectin by leukocytes and changes in acute-phase reactants in total hip replacement surgery. Eur Surg Res 1996;28:190.

11.

Quarmby J , Smith A , Collins M , et al. A model of in vivo human venous thrombosis that confirms changes in the release of specific soluble cell adhesion molecules in experimental venous thrombogenesis. J Vasc Surg 1999;30:139.

12.

Smith A , Quarmby JW , Collins M , et al. Changes in the levels of soluble adhesion molecules and coagulation factors in patients with deep vein thrombosis. Thromb Haemost 1999;82:1593.

13.

Caimi G , Canino B , Ferrara F , et al. Leukocyte rheology before and after chemotactic activation in some venous diseases. Eur J Vasc Endovasc Surg 1999;18:411.

14.

Saharay M , Shields DA , Porter JB , et al. Leukocyte activity in the microcirculation of the leg in patients with chronic venous disease. J Vasc Surg 1997;26:265.