Background: It has been suggested that cerebral blood vessels and brain cells might depend more on intracellular calcium than extracellular calcium to modulate intracellular free calcium concentrations, [Ca2+]i.
Methods and Results: A potent intracellular calcium antagonist, dantrolene, was used to prevent the ischemic stroke induced in the rat model. It was found that treatment of rats with dantrolene at —1 hour and +1 hour after 60 minutes of ischemic insult prevented by the formation of cortical necrosis 98% and 85%, respectively. Further, the [Ca2+]i of embry onic aorta cells was markedly reduced, and cAMP of the same cultured cells were signifi cantly increased by dantrolene treatment.
Conclusions: These results indicate that ischemic stroke is preventable by dantrolene through reduction of [Ca2+]i and increase of cAMP.
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