Objective:We determined the level of message for fibrillin-1, a single-stranded glycoprotein found in the elastic micropfibrils of the extracellular matrix, in human placenta compared with other adult human tissues and localized the protein int he term human placenta.
Methods:Northern blot analysis using a cDNA specific for fibrillin-1 was used to compare message levels in several adult human tissues and placenta. Immunohistochemical staining, using a monoclonal antibody to human fibrillin-1, was used to visualize the sitz of fibrillin-1 in term human placenta.
Results:Larger amounts of message for fibrillin-1 were present in placenta than in any of the other tissues tested. Staining for fibrillin-1 was present in the villous stroma but not in the basement membranes, trophoblast cells, or in blood vessels of stem, mature intermediate, or terminal villi.
Conclusions:Fibrillin-1 is distributed extensively in the villous stroma of the term human placenta and may contribute elastic properties to the placenta and basal plate required for an active uterus.
1. Sakai LY, Kenne DR, Engvall E. Fibrillin, a new 350-kD glycoprotein, is a component of extracellular microfibrils. J Cell Biol1986;103:2499-2509.
2.
2. Dietz HC, Cutting GR, Pyeritz RE, et al. Marfan syndrome caused by a recurrent de novo missense mutation in the fibrillin gene. Nature1991;352:337-339.
3.
3. Maslen CL, Corson GM, Maddox BK, Glanville RW, SAkai LY. Partial sequence of a candidate for the Marfan syndrome. Nature1991;352:334-337.
4.
4. Maslen CL, Glanville RW. The Marfan syndrome. The Endocrinologist1991;3:279-287.
5.
5. Low FN. Microfibrils: Fine filmentous components of the tissue space. Anat Rec1962;142:131-137.
6.
6. Keene DR, Maddox BK, Kuo H-J, Sakai LY, Glanville RW. Extraction of extendible beaded structures and their identification as fibrillin-containing extracellular matrix microfibrils. J Histochem Cytochem1991;39:441-449.
7.
7. Fleischmajer R, Contard P, Schwartz E, MacDonald ED Ii, Jacobs L II, Sakai LY. Elstin-associated microfibrils (10 nm) in a three-dimensional fibroblast culture. J Invest Dermatol1991;97:638-643.
8.
8. Mecham RP, Heuser JE. The elastic fiber. In: Hay ED, ed. Cell biology of extracellular matrix. 2nd ed.New York: Plenum, 1991:79-109.
9.
9. Arbeille BB, Fauvel-Lafeve FMJ, Lemesle MB, Tenza D, Legrand YJ. Thrombospondin: A component of microfibrils in various tissues. J Histochem Cytochem1991; 39:1367-1375.
10.
10. Autio-Harmainen H, Hurskainen T, Niskasaari K, Hoeyhtyae M, Tryggvason K. Simultaneous expression of 70 kilodalton type IV collagenese and type IV collagen α1 (IV) chain genes by cells of early human placenta and gestational endometrium. Lab Invest1992;67:191-200.
11.
11. Castellucci M, Classen-Linke I, Muehlhauser J, et al. The human placenta: A model for tenascin expression. Histochemistry1991;95:449-458.
12.
12. Earl U, Estlin C, Bulmer JN. Fibronectin and laminin in the early human placenta. Placenta1990;11:223-231.
13.
13. Juvonen M, Pihlajaniemi T, Autio-Harmainen H. Location and alternative splicing of type XIII collagen RNA in the early human placenta. Lab Invest1993;69:541-551.
14.
14. Nanaev AK, Rukosuev VS, Shirinsky VP, et al. Confocal and conventional immunofluorescent and immunogold electron microscopic localization of collagen types III and IV in human placenta. Placenta1991;12:573-595.
15.
15. Shen G-Q, Butkowski R, Cheng T, et al. Comparison of non-collagenous type IV collagen subunits in human glomerular basement membrane, alvelolar basement membrane, and placenta. Connect Tissue Res1990;24:289-301.
16.
16. Schuppan D, Cantaluppi MC, Becker J, et al. Undulin, an extracellular matrix glycoprotein associated with collagen fibrils. J Biol Chem1990;265:8823-8832.
17.
17. Rukosuev VS. Immunofluorescent localization of collagen types I, III, IV, V, fibronectin, laminin, entactin, and heparan sulphate proteoglycan in human immature placenta. Experientia1992;48:285-287.
18.
18. Amenta PS, Gay S, Vaheri A, Martinez-Hernandez A. The extracellular matrix is an integrated unit: Ultrastructural localization of collagen types I, III, IV, V, VI, fibronectin and laminin in human term placenta. Collagen Res Rel1986;6:125-152.
19.
19. Maddox BK, Sakai LY, Keene DR, Glanville RW. Connective tissue microfibrils. Isolation and characterization of three large pepsin-resistant domains of fibrillin-1. J Biol Chem1989;264:21381-21385.
20.
20. Benirschke K, Kaufmann P. Pathology of the human placenta. 2nd ed.New York: Springer-Verlag, 1990:270-278.
21.
21. Glanville RW, Qian R-Q, McClure DW, Maslen CL. Calcium binding, hydroxylation and glycosylation of the precursor epidermal growth factor-like domains of fibrillin-1, the Marfan gene protein. J Biol Chem1994;269:26630-26634.
