Maternal Serum Leptin Concentrations Do Not Correlate With Cord Blood Leptin Concentrations in Normal Pregnancy

Abstract

Objective: To determine whether there is a difference in maternal leptin concentration and cord blood concentration, consistent with the hypothesis of a noncommunicating, two-compartement model of fetoplacental leptin regulation.

Methods: Blood samples were collected from 139 women, identified as having an uncomplicated pregnancy, from an antecubital vein at delivery. Cord blood samples were taken from the umbilical vein. Leptin was measured by radioimmunoassay, and its relationship to fetal and maternal anthropometrics was assessed by Spearman correlation. Differences in maternal and cord blood leptin levels between male and female infants were tested twith the Mann-Whitney U test. Maternal and cord blood leptin were compared by the Wilcoxon signed rank test. The outcome measures were maternal and cord blood leptin at delivery, fetal birth weight, length, weight/length ratio, and ponderal index, maternal prepregnancy body mass index, pregnancy weight gain, relative weight gain, and body mass index at delivery.

Results: No correlations were found between maternal and cord blood leptin concentrations. Fetal leptin level correlated with birth weight (ρ = 0.665; p < .0001), length (ρ = 0.490; P < .0001), ponderal index (ρ = 0.260; P = .002), and weight/length ratio (ρ = 0.625; P < .0001). Median leptin concentrations were higher in female (9.3 ng/mL, range 1.5-34.4 ng/mL) than in male (8.2 ng/mL, range 1.6-38.3 ng/mL) neonates, but this difference was statistically not significant. Logistic regression analysis showed a significant influence on umbilical venous leptin concentration for birth weight (P < .0001) but not for gender. Maternal leptin concentrations were significantly higher than cord leptin concentrations (P < .0005 for the male and female neonates and the entire group).

Conclusion: There was no correlation between maternal and cord leptin, whiich supports the hypothesis of a noncommunicating, two-compartment model of fetoplacental leptin regulation.

Keywords

Maternal leptin cord blood leptin pregnancy anthropometric measurements

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References

1. Zhang Y , Proenca R , Maffei M , Barone M , Leopold L , Friedman JM . Positional cloning of the mouse obese gene and its human homologue. Nature 1994;372:425-432.

2. Pelleymounter MA , Cullen MJ , Baker M , et al. Effects of the obese gene product on body weight regulation in ob/ob mice. Science 1995;269:540-543.

3. Halaas JL , Gajiwala KS , Maffei M , et al. Weight-reducing effects of the plasma protein encoded by the ob gene. Science 1995;269:543-546.

4. Campfield LA , Smith F , Guisez Y , Devos R , Burn P . Recombinant mouse OB protein: Evidence of a peripheral signal linking adiposity and central neural networks. Science 1995;269:546-549.

5. Considine RV , Sinha MK , Heiman ML , et al. Serum immunoreactive leptin concentrations in normal-weight and obese humans. N Engl J Med 1996;334:292-295.

6. Hassink SG , Shelsow DV , de Lancey E , Opentanova I , Considine RV , Caro JF . Serum leptin in children with obesity: Relationship to gender and development. Pediatrics 1996;98:201-203.

7. Clapp JF III , Kiess W . Cord Blood leptin reflects fetal fat mass. J Soc Gynecol Investig 1998;5:300-303.

8. Schubring C , Kiess W , Englaro P , Rascher W , Blum W . Leptin concentrations in amniotic fluid, venous and arterial cord blood and maternal serum: High leptin synthesis in the ferns and inverse correlation with placental weight. Eur J Pediatr 1996;155:830-830.

9. Hassink SG , de Lancey E , Shelsow DV , et al. Placental leptin: An important new growth factor in intrauterine and neonatal development? Pediatrics 1997;100:1-9.

10.

10. Senaris R , Garcia-Caballero T , Casabiell X , et al. Synthesis of leptin in human placenta. Endocrinology 1997;138:4501-4504.

11.

11. McCarthy JF , Misra DN , Roberts JM . Maternal plasma leptin is increased in preeclampsia and positively correlates with fetal cord concentration. AM J Obstet Gynecol 1999;180:731-736.

12.

12. Schubring C , Kiess W , Englaro P , et al. Levels of leptin in maternal serum, amniotic fluid, and arterial and venous cord blood: Relation to neonatal and placental weight. J Clin Endocrinol Metab 1997;82:1480-1483.

13.

13. Matsuda J , Yokota I , Iida M , et al. SErum leptin concentration in cord blood: Relationship to birth weight and gender. J Clin Endocrinol Metab 1997;82:1642-1644.

14.

14. Catalano PM , Tyzbir ED , Allen Sr , McBean JH , McAuliffe TL . Evaluation of fetal growth by estimation of fetal body composition. Obstet Gynecol 1992;79:46-50.

15.

15. Clapp JF , Capeless EL . Neonatal morphometrics after endurance exercise during pregnancy. Am J Obstet Gynecol 1990;163:1805-1811.

16.

16. Sivan E , Lin WM , Homko Cj , Reece A , Boden G . Leptin is present in human cord blood. Diabetes 1997;46:917-919.

17.

17. Koistinen HA , Koivisto VA , Anderson S , et al. Leptin concentration in cord blood correlates with intrauterine growth. J Clin Endocrinol Metab 1997;82:3328-3330.

18.

18. Blum WF , Englaro P , Hanitsch S , et al. Plasma leptin levels in healthy children and adolescents: Dependence on body mass index, body fat mass, gender, pubertal stage, and testosterone. J Clin Endocrinol Metab 1997;82:2904-2910.

19.

19. Kolacznski JW , Nyce MR , Considine RV , et al. Acute and chronic effects of insulin on leptin production in humans: Studies in vivo and in vitro. Diabetes 1996;45:699-701.

20.

20. Havel PJ , Kasim-Karakas S , Dubuc GR , Mueller W , Phinney SD . Gender differences in plasma leptin concentrations. Nat Med 1996;2:949-950.

21.

21. Catalano PM , Drago NM , Amini SB . Factors affecting fetal growth and body composition. Am J Obstet Gynecol 1959;172:1459-1463.

22.

22. Tannura T , Goldenberg RL , Johnston KE , Cliver SP . Serum leptin concentrations during pregnancy and their relationship to fetal growth. Obstet Gynecol 1998;91:389-395.

23.

23. Butte NF , Hopkinson JM , Nicolson MA . Leptin in human reproduction: Serum leptin levels in pregnant and lactating women. J Clin Endocrinol Metab 1997;82:585-589.

24.

24. Highman TJ , Friedman JE , Huston LP , Wong WW , Catalano PM . Longitudinal changes in maternal serum leptin concentrations, body composition, and resting metabolic rate in pregnancy. Am J Obstet Gynecol 1998;178:1010-1015.

25.

25. Carmichael SL , Abrams B . A critical review of the relationship between gestational weight gain and preterm delivery. Obstet Gynecol 1997;89:865-873.

26.

26. Wen SW , Goldenberg RL , Cutter GR , Hoffman HJ , Cliver SP . Intrauterine growth retardation and preterm delivery: Prenatal risk factors in an indigent population. Am J Obstet Gynecol 1990;162:213-218.

27.

27. Masuzaki H , Ogawa Y , Sagawa N , et al. Nonadipose tissue production of leptin: Leptin as a novel placenta-derived hormone in humans. Nat Med 1997;3:1029-1033.

28.

28. Cioffi JA , Shafer AW , Zupancic TJ , et al. Novel B219/OB receptor isoforms: Possible role of leptin in hematopoiesis and reproduction. Nat Med 1996;2:585-589.