Role of Polymerase Chain Reaction in the Diagnosis of Cytomegalovirus Infection in Liver Transplant Patients

Abstract

Eighty-eight formalin-fixed, paraffin-embedded needle biopsies from 58 liver trans plant recipients were analyzed for the presence of cytomegalovirus (CMV) by light microscopy and the polymerase chain reaction (PCR). Twenty-seven biopsies were positive for CMV by both light microscopy and PCR, 41 were negative by both methods, 17 were positive by PCR only, and 3 were positive by light microscopy only. In the absence of cytomegalic cells, immunohistochemical staining was unable to detect CMV that could have been identified by PCR. Serum total bilirubin was higher in patients whose biopsies contained PCR (but not histologic) evidence of CMV infection. No evidence of association of the presence of CMV was found by either PCR or light microscopy with serum levels of aminotransferase or alkaline phosphatase, nor with histologic evidence of rejection or hepatitis. PCR was negative for CMV in 22 liver biopsies from immunocompetent individuals without evidence of hepatic dysfunction. Although PCR can detect the presence of CMV in the absence of cytomegalic cells, the clinical significance of PCR-proven, histologically undetectable CMV in the liver is undetermined. Int J Surg Pathol 2(3):221-226, 1995