The expression of integrins α2β1, α3β1, and α5β1 in 30 ameloblastomas (20 solid and 10 unicystic tumors), 12 adenomatoid odontogenic tumors (AOTs), and 5 human tooth germs in different stages of odontogenesis was analyzed. The distribution, location, pattern, and intensity of immunohistochemical expression were evaluated. Intensity was analyzed using scores (0 = absence, 1 = weak staining, and 2 = strong staining). No difference in the immunoexpression of the integrins was observed between solid and unicystic ameloblastomas. When these two ameloblastoma types were pooled into a single group, the following significant differences were found: immunoexpression of integrin α2β1 was stronger in ameloblastomas than in AOTs and tooth germs, and the expression of integrin α5β1 was stronger in ameloblastomas than in AOTs. The lack of detection of integrin α3β1 in tooth germs and its detection in the odontogenic tumors studied suggest that this integrin might be used as a marker of neoplastic transformation in odontogenic tissues.
Santos JN, Pereira Pinto L., de Figueredo Crlv, de Souza LBOdontogenic tumors: analysis of 127 cases. Pesqui Odontol Bras. 2001;15:308-313.
4.
Philipsen HP , Reichart PAUnicystic ameloblastoma. A review of 193 cases from the literature. Oral Oncol.1998; 34:317-325.
5.
Barnes L., Eveson JW, Reichart P., Sidransky D.World Health Organization Classification of Tumours. Pathology and Genetics of Head and Neck Tumours. Lyon , France: IARC Press; 2005.
Mosqueda-Taylor A., Ledesma-Montes C., Caballero-Sandoval S., Portilla-Robertson J., Ruiz-Godoy Rivera LM, Meneses-Garcia A.Odontogenic tumors in Mexico. A collaborative retrospective study of 349 cases . Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997;48: 672-675.
8.
Heikinheimo K. , Morgan PR, Happonen RP, Stenman G., Virtanen I.Distribution of extracellular matrix proteins in odontogenic tumours and developing teeth . Virchows Arch B Cell Pathol. 1991;61:101-109.
9.
Medeiros AMC.Expressão da fibronectina, tenascina e colágeno I em ameloblastomas e no tumor odontogênico adenomatóide [Tese, Doutorado em Patologia Oral]. Natal-RN, Brasil: Departamento de Odontologia, Universidade Federal do Rio Grande do Norte; 2001.
10.
Yokosaki Y., Monis H., Chen J., Sheppard D.Differential effects of the integrins α9β1, αvβ3, and αvβ6 on cell proliferative response to tenascin. Roles of the β subunit extracellular and cytoplasmic domains. J Biol Chem.1996 ;271:24144-24150.
11.
Miles AJ, Knutson JR, Skubitz AP, Furcht LT, Mccarthy JB, Fields GBA peptide model of basement membrane collagen α1 (IV) 531-543 binds the α3β1 integrin. J Biol Chem. 1995;270:29047-29050.
12.
Van Waes C. , Surh DM, Chen Z., et al. Increase in suprabasilar integrin adhesion molecule expression in human epidermal neoplasms accompanies increased proliferation occurring with immortalization and tumor progression . Cancer Res. 1995;55:5434-5444.
13.
Franchi A., Santoro R., Paglierani M., Bondi R.Comparison of integrin α chain expression in benign and malignant salivary gland tumours. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1997;83:588-595.
14.
Benassi MS, Ragazzini P., Gamberi G., et al. Adhesion molecules in high-grade soft tissue sarcomas: correlation to clinical outcome. Eur J Cancer. 1998;34:496-502.
15.
Hynes ROIntegrins: a family of cell surface receptors. Cell . 1987;48:549-554.
16.
Hynes ROIntegrins: versatility, modulation, and signaling in cell adhesion . Cell. 1992;69:11-25.
17.
Thomas GJ, Jones J., Speight PMIntegrins and oral cancer. Oral Oncol. 1997 ;33:381-388.
18.
Kreidberg JAFunctions of α3β1 integrin. Curr Opin Cell Biol. 2000;12:548-555.
19.
Souza LB, Sousa CVC, Pereira Pinto L.Estudo morfológico e imuno-histoquímico da membrana basal em diversos padrões histológicos do ameloblastoma. Rev Pós Grad. 2001;8:40-45.
20.
Di Persio CM , Hodivala-Dilke KM, Jaenish R., Kreidberg JA, Hynes ROα3β1 Integrin is required for normal development of the epidermal basement membrane. J Cell Biol . 1997;137:729-742.
21.
Labat-Robert J.Fibronectin in malignancy. Effect of aging . Semin Cancer Biol. 2002;12:187-195.
22.
Damsky CH, Ilic D.Integrin signaling: it's where the action is. Curr Opin Cell Biol. 2002;14:594-602.
23.
Kumamoto H. , Kimi K., Ooya K.Detection of cell cycle-related factors in ameloblastomas. J Oral Pathol Med. 2001;30:309-315.
24.
Kumamoto H. , Kimi K., Ooya K.Immunohistochemical analysis of apoptosis-related factors (Fas, Fas ligand, caspase-3 and single-stranded DNA) in ameloblastomas . J Oral Pathol Med. 2001;30:596-602.
25.
Sandra F., Mitsuyasu T., Nakamura N., Shiratsuchi Y., Ohishi M.Immunohistochemical evaluation of PCNA and Ki-67 in ameloblastoma . Oral Oncol. 2001;37:193-198.
26.
Sandra F., Nakamura N., Kanematsu T., Hirata M., Ohishi M.The role of MDM2 in the proliferative activity of ameloblastoma. Oral Oncol. 2002;38:153-157.
27.
Coppolino MG , Deddhar S.Bi-directional signal transduction by integrin receptors. Int J Biochem Cell Biol. 2000;32:171-188.
28.
Okajian GK, Schwimmer R.Regulation of epithelial cell surface polarity reversal by β1 integrins . J Cell Sci. 1994;107:561-576.
29.
Murata M., Cheng J., Horino K., Hara K., Shimokawa H., Saku T.Enamel proteins and extracellular matrix molecules are co-localized in the pseudocystic stromal space of adenomatoid odontogenic tumor. J Oral Pathol Med. 2000;29:483-490.
30.
Heikinheimo K., Salo T.Expression of basement membrane type IV collagen and type IV collagenases (MMP-2 and MMP-9) in human fetal teeth. J Dent Res. 1995; 74:1226-1234.
31.
Thesleff I. , Partanen AM, Vainio S.Epithelial-mesenchymal interactions in tooth morphogenesis: the roles of extracellular matrix, growth factors, and cell surface receptors. J Craniofac Genet Dev Biol. 1991;11:229-237.
32.
Sawada T., Nanci A.Spatial distribution of enamel proteins and fibronectin at early stages of rat incisor tooth formation. Arch Oral Biol. 1995;40:1029-1038.
33.
Nykvist P., Tu H., Ivaska J., Kapyla J., Pihlajaniemi T., Heino J.Distinct recognition of collagen subtypes by α1β1 and α2β1 integrins. J Biol Chem. 2000;275: 8255-8261.
34.
Brown EJIntegrin-associated proteins. Curr Opin Cell Biol . 2002; 14:603-607.
35.
Mitra A., Chakrabarti J., Banerji A., Chatterjee A.Binding of α2 monoclonal antibody to human cervical tumor cell (SiHa) surface α2β1 integrin modulates MMP-2 activity. Gynecol Oncol. 2004;94:33-39.
Pinheiro JJ , Freitas VM, Moretti AI, Jorge AG, Jaeger RGLocal invasiveness of ameloblastoma. Role played by matrix metalloproteinases and proliferative activity. Histopathology. 2004;45:65-72.
Giannelli G. , Fransvea E., Marinosci F., et al. Transforming growth factor-β1 triggers hepatocellular carcinoma invasiveness via α3β1 integrin. Am J Pathol. 2002;161:183-193.
