Stimulation of β-adrenergic receptors by isoproterenol or addition of 8-BrcAMP rapidly and transiently induces the expression of the protooncogenes, c-fos, and jun B, but not that of c-jun in A5 cells. These results indicate that different intracellular pathways may operate within the same cell for the induction of this group of early response genes. The inducibility of c-fos and jun B genes by either isoproterenol of 8-BrcAMP is transcriptionally regulated and accompanied by increases in their respective products. Furthermore, both c-fos and jun B mRNA levels are elevated at G0/G 1 phase of the A5 cell cycle and are inducible by isoproterenol or 8-BrcAMP at the different phases of the cell cycle. These data further suggest a possible role of c-fos and jun B in A5 cell cycle.
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