Biochemical approaches were utilized initially to identify the virulence factors of the mutans streptococci (primarily Streptococcus mutans and S. sobrinu). Traditional mutant analysis of these organisms further suggested the important role of several of these factors in cariogenicity. However, because these mutations were not clearly defined, the utilization of cloned genes was necessary to verify their significance. The introduction of molecular genetic approaches for characterizing these factors has led not only to a clearer understanding of the role of these virulence factors in cariogenicity but has also suggested some novel approaches for reducing further the incidence of dental caries.
References
1.
Abo, H., T. Matsumura, T. Kodama, H. Ohta, K. Fukui, K. Kato, and H. Kagawa: Peptide Sequences for Sucrose Splitting and Glucan Binding Within Streptococcus sobrinus Glucosyltransferase (Water-insoluble Glucan Synthetase). J. Bacteriol. 173:989-996 (1991).
2.
Ackermanns, F., J.P. Klein, J.A. Ogier, H. Bazin, F. Cormont, and R.M. Frank: Purification and Characterization of a Saliva-interactive Cell Wall Protein from Streptococcus mutans Serotype f by Using Monoclonal Antibody Immunoaffinity Chromatography. Biochem. J. 228:211-217 (1985).
3.
Aoki. H., T. Shiroza, M. Hayakawa, S. Sato, and H.K. Kuramitsu: Cloning of a Streptococcus mutans Gene Coding for Insoluble Glucan Synthesis. Infect. Immun. 53:587-594 (1986).
4.
Banas, J.J. and K.S. Gilmore: Analysis of Streptococcus mutans and Streptococcus downei Mutants Insertionally Inactivated in the gbp and gtfs Genes. In: Genetics and Molecular Biology of Streptococci. Lactococci. and Enterococci, pp. 281-283. (G. M. Dunny, P. P. Cleary, and L. L. McKay, Eds.) Amer. Soc. Microbiol, Washington, D.C. (1991 ).
5.
Barrett. J. F., T.A. Barrett, and R. Curtiss, III: Purification and Partial Characterization of the Multicomponent Dextranase Complex of Stretptococcus sobrinus and Cloning of the Dextranase Gene. Infect. Immun. 55:792-802 (1987).
6.
Barletta, R.G., S.M. Michalek, and R. Curtiss III: Analysis of the Virulence of Streptococcus mutans Serotype c Mutants in the Rat Model System. Infect. Immun. 56:322-330 (1988).
7.
Bender. G. R. S.V. Sutton, and R.E. Marquis: Acid Tolerance, Proton Permeabilities, and Membrane ATPases of Oral Streptococci. Infect. Immun. 53:331-338 (1986).
8.
Bowen, W.H. K.M. Madison, and S.K. Pearson: Influence of Desalivation in Rats on Incidence of Caries in Intact Cagemates, J. Dent. Res. 67:1316-1318 (1988 ).
9.
Bowen, W.H. K. Schilling, E. Giertsen, S. Pearson, S.F. Lee,A.S. Bleiweis, and D. Beeman: Role of a Cell Surface-Associated Protein in Adherence and Dental Caries. Infect. Immun. 59:4606-4609 (1991 ).
10.
Bume, R.A. D.T. Parsons, and R.E. Marquis: Cloning and Expression in Escherichi coli of the Genes of the Arginine-deiminase System of Streptococcus sanguis NCTC 10904. Infect. Immun. 57:3540-3548 (1989).
11.
Burne, R.A. B. Rubenfeld, W.H. Bowen, and R.E. Yasbin: Tight Genetic Linkage of a Glucosyltransferase and Dextranase of Streptococcus mutans GS-5. J. Dent. Res. 65:1392-1401 (1986).
12.
Bume, R.A., K.M. Schilling, W.H. Bowen, and R.E. Yasbin: Expression, Purification, and Characterization of an Exo-β-D-fructosidase of Streptococcus mutans, J. Bacteriol. 169:4507-4511 (1987).
13.
Carlsson, J.: A Levansucrase from Streptococcus mutans. Caries Res. 4:97-113 (1970).
14.
Caufield, P.W., G.R. Shah, and S.K. Hollingshead: Use of Transposon Tn916 to Inactivate and Isolate a Mutacinassociated Gene from Streptococcus mutans. Infect. Immun. 58:4126-4135 (1990).
15.
Caufield, P.W. and T.M. Walker: Genetic Diversity within Streptococcus mutans Evident from Chromosomal DNA Restriction Fragment Polymorphisms. J. Clin. Microbiol. 27:274-278 (1989).
16.
Chassy, B.: Sucrose Metabolism and Glucosyltransferase in Oral Streptococci. In: Glucosyltransferases. Glucans, Sucrose, and Dental Caries, pp. 3-10. (R. J. Doyle and C. E. Ciardi, Eds.) IRL Press, Washington, D.C. (1983).
Curtiss, R. III: Genetic Analysis of Streptococcus mutans Virulence. Curr. Top. Microbiol. Immunol.118:253-277 (1985).
19.
Curtiss, R. III, R.M. Goldschmidt, N.B. Fletchall, and S.M. Kelly: Avirulent Salmonella typhimurium delta cya delta crp Oral Strains Expressing a Streptococcal Colonization and Virulence Antigen. Vaccine. 6:155-160 (1988).
20.
Curtiss, R. III, R. Goldschmidt, R. Pastina, M. Lyons, S.M. Michalek, and J. Mestecky: Cloning Virulence Determinants from Streptococcus mutans and the use of Recombinant Clones to Construct Bivalent Oral Vaccine Strains to Confer Protective Immunity Against S. mutans-lnduced Dental Caries. In: Molecular Microbiology and Immunobiology of Streptococcus mutans. pp. 173-180. ( S. Hamada, S. M. Michalek, H. Kiyono, L. Menaker, and J. R. McGhee, Eds.) Elsevier Science. Amsterdam: ( 1986 ).
21.
Curtiss, R. III, S.A. Larrimore, R.G. Holt, J.F. Bamett. R. Barletta, H.H. Murchison, S.M. Michalek, and S. Saito: Analysis of Streptococcus mutans Virulence Attributes using Recombinant DNA and Immunological Techniques. In: Glucosyltransferases, Glucans, Sucrose. and Dental Caries. pp. 95-104. (R. J. Doyle, and J. E. Ciardi. Eds.) IRL Press, Arlington (1983).
22.
Demuth, D.R., C.A. Davis, A.M. Comer, R.J. Lamont. R.S. Leboy, and D. Malamud: Cloning and Expression of a Streptococcus sanguis Surface Antigen That Interacts with a Human Salivary Agglutinin. Infect. Immun. 56:2484-2490 (1988).
23.
Dertzbaugh, M.T., D.L. Peterson, and F.L. Macrina: Cholera Toxin B-Subunit Gene Fusions: Structural and Functional Analysis of the Chimeric Protein. Infect. Immun. 58:70-79 (1990).
24.
de Stoppelaar, J.D., K. Konig, A. Plasschaert, and J. van der Hoeven: Decreased Cariogenicity of a Mutant of Streptococcus mutans. Arch. Oral Biol. 16:971-975 (1971).
25.
DiRita, V.J. and J.J. Mekalonos: Genetic Regulation of Bacterial Virulence. Annu. Rev. Genet. 23:455-482 ( 1989).
26.
Donoghue, H.D. and H.N. Newman: Effect of Glucose and Sucrose on Survival in Batch Culture of Streptococcus mutans C67-25. Infect. Immun. 13:16-21 (1976).
27.
Douglas, C.W.I. and R.R.B. Russell: Effects of Specific Antisera Upon Streptococcus mutans Adherence to Saliva-Coated Hydroxyapatite. FEMS Microbiol. Lett. 25:211-214 (1984).
28.
Drake, D., K.R. Taylor, A.S. Bleiweis and R.J. Doyle: Specificity of the Glucan-binding Lectin of Streptococcus cricetus. Infect. Immun. 56:1864-1872 (1988 ).
29.
Duncan, M.J. and J.D. Hillman: DNA Sequence and in vitro Mutagenesis of the Gene Encoding the Fructose-1-6-diphosphate-dependent L-(+) Lactate Dehydrogenase of Streptococcus mutans. Infect. Immun. 59:3930-3934 (1991).
30.
Ellwood, D.C.: Chemostat Studies of Oral Bacteria. In: Microbial Aspects of Dental Caries. Vol. 3, pp. 785-798. (H. M. Stiles, W. J. Loesche, and T. C. O'Brien, Eds.) IRL Press, Washington, D.C. (1976).
31.
Ferretti, J.J., M.L. Gilpin, and R.R.B. Russell: Nucleotide Sequence of a Glucosyltransferase Gene from Streptococcus sobrinus MFe28. J. Bacteriol. 169:4271-4278 (1987 ).
32.
Fitzgerald, R.J., B.O. Adams, H.J. Sandham, and S. Abhyanker: Cariogenicity of a Lactate Dehydrogenase-Deficient Mutant of Streptococcus mutans Serotype c in Gnotobiotic Rats. Infect. Immun. 57:823-826 (1989).
33.
Fitzgerald, R.J. and P.H. Keyes: Demonstration of the Etiologic Role of Streptococci in Experimental Caries in the Hamster. J. Am. Dent. Assoc. 61:9-19 (1960).
34.
Freedman, M.L. and J.M. Tanzer: Dissociation of Plaque Formation from Glucan-Induced Agglutination in Mutants of Streptococcus mutans. Infect. Immun. 10:189-196( 1974).
35.
Freedman, M.L., J.M. Tanzer, and A.L. Coykendall: The Use of Genetic Variants in the Study of Dental Caries. In: Animal Models in Cariology (Spec. Suppl. to Microbiol. Abstr.-Bacteriol.), pp. 247-269. (J. M. Tanzer, Ed.) Information Retrieval, Washington, D.C. (1981).
36.
Fukushima, K., R. Motoda, K. Takada, and T. Ikeda: Resolution of Streptococcus mutans Glucosyltransferases into Two Components Essential to Water-Insoluble Glucan Synthesis. FEBS Lett. 128:213-216 (1981).
37.
Gibbons. R. J.: Bacterial Adhesion to Oral Tissue: A Model for Infectious Diseases. J. Dent. Res. 68:750-760 (1989).
38.
Gibbons, R.J., K.S. Berman, P. Knoettner, and B. Kapsimalis: Dental Caries and Alveolar Bone Loss in Gnotobiotic Rats Infected with Capsule-forming Streptococci of Human Origin. Arch. Oral Biol. 11:549-560 (1966).
39.
Gibbons, R.J., L. Cohen, and D.I. Hay: Strains of Streptococcus mutans and Streptococcus sobrinus Attach to Different Pellicle Receptors. Infect. Immun. 52:555-561 (1986).
40.
Gibbons, R.J. and I. Etherden: Comparative Hydrophobicities of Oral Bacteria and their Adherence to Salivary Pellicles. Infect. Immun. 41:1190-1196 (1983).
41.
Gibbons, R.J. and R.J. Fitzgerald: Dextran-Induced Agglutination of Streptococcus mutans and its Potential Role in the Formation of Microbial Dental Plaques. J. Bacteriol. 98:341-346 (1969).
42.
Gilmore, K., R.R.B. Russell, and J.J. Ferretti: Analysis of a Streptococcus downei gtfs Gene which Specifies a Glucosyltransferase that Synthesizes Soluble Glucan. Infect. Immun. 58:2452-2458 (1990).
43.
Gilpin, M.L., R.R.B. Russell, and P. Morrissey: Cloning and Expression of Two Streptococcus mutans Glucosyltransferases in Escherichia coli. Infect. Immun. 49:414-416 (1985).
44.
Glass, R.L.: Introduction: The First International Conference on the Declining Prevalence of Dental Caries. J. Dent. Res. 61:1304 (1982).
45.
Guggenheim, B., B. Reolati, and H.R. Muhleman: Caries and Plaque Inhibition by Mutanase in Rats. Caries Res. 6:289-297 (1972).
46.
Hamada, S. and T. Ooshima: Production and Properties of Bacteriocins (Mutacins) from Streptococcus mutans. Arch. Oral Biol. 20:641-648 (1975).
47.
Hamada, S. and H.D. Slade: Biology, Immunology, and Cariogenicity of Streptococcus mutans. Microbiol. Rev. 44:331-384 (1980).
48.
Hanada, N. and H.K. Kuramitsu: Isolation and Characterization of the Streptococcus mutans gtfC Gene Coding for the Synthesis of Both Soluble and Insoluble Glucans. Infect. Immun. 56:1995-2005 (1988).
49.
Hanada, N. and H.K. Kuramitsu: Isolation and Characterization of the Streptococcus mutans gtfD Gene Coding for Primer-Dependent Soluble Glucan Synthesis. Infect. Immun. 57:2079-2085 (1989).
50.
Hanada, N., Y. Yamashita, Y. Shibata, S. Sato, T. Katayama, T. Takehara, and M. Inoue: Cloning of a Streptococcus sobrinus gtf Gene that Encodes a Glucosyltransferase which Produces a High Molecular Weight Water-Soluble Glucan. Infect. Immun. 59:3434-3438 (1991).
51.
Harper, D.S. and W.J. Loesche: Growth and Acid Tolerance of Human Dental Plaque Bacteria. Arch. Oral Biol. 29:843-848 (1984).
52.
Harris, G.S. and R. Curtiss III.: Cloning of a Locus Involved in Streptococcus mutans Glycogen Accumulation. Abstr. Am. Soc. Microbiol. No. D-89 (1991).
53.
Hayakawa, M., H. Aoki, and H.K. Kuramitsu: Isolation and Characterization of the Sucrose-6-Phosphate Hydrolase Gene from Streptococcus mutans. Infect. Immun. 53:582-586 (1986).
54.
Hillman, J.D.: Lactate Dehydrogenase Mutants of Streptococcus mutans: Isolation and Preliminary Characterization. Infect. Immun. 21:206-212 (1978).
55.
Hillman, J.D., M.J. Duncan, and K.P. Stashenko: Cloning and Expression of the Gene Encoding the Fructose-1-6-diphosphate-dependent L-(+) Lactate Dehydrogenase of Streptococcus mutans. Infect. Immun. 58:1290-1295 (1990).
56.
Hillman, J.D., A.L. Dzubach, and S.W. Andrews: Colonization of the Human Oral Cavity by a Streptococcus mutans Mutant Producing Increased Bacteriocin. J. Dent. Res. 66:1092-1094 (1987).
57.
Holt, R.G., Y. Abiko, S. Saito, M. Smorawinska, J.B. Hansen, and R. Curtiss III: Streptococcus mutans Genes That Code for Extracellular Proteins in Escherichia coli. Infect. Immun. 38:147-156 (1982).
58.
Hudson. M. C. and R. Curtiss III: Regulation of Expression of Streptococcus mutans Genes Important to Virulence. Infect. Immun. 58:464-470 (1990).
59.
Koga. T., S. Sato, M. Inoue, K. Takeuchi, T. Furuta, and S. Hamada: Role of Primers in Glucan Synthesis by Glucosyltransferases from Streptococcus mutans OMZ-176. J. Gen. Microbiol. 129:751-754 (1983).
60.
Kohler, B. and B. Krasse: Human Strains of Mutans Streptococci Show Different Cariogenicity, J. Dent. Res.67 (Spec. Issue):343 (1988).
61.
Krasse, B.: Caries Risk. Quintessence, Chicago (1985).
62.
Kriger, P.S. and R.G. Quivey: Purification of a Mutanase, J. Dent. Res.69 (Spec. Issue):325 (1990).
63.
Kuramitsu, H.K. and L. Wondrack: Insoluble Glucan Synthesis by Streptococcus mutans Serotype c Strains. Infect. Immun. 42:763-770 (1983).
64.
Lee, S.F. A. Progulske-Fox, and A.S. Bleiweis: Molecular Cloning and Expression of a Streptococcus mutans Major Surface Protein Antigen PI (I/II) in Escherichia coli. Infect. Immun. 56:2114-2119 (1988).
65.
Lee, S.F. A. Progulske-Fox, G.W. Erdos, D.A. Placentini, G.W. Ayakawa, P.J. Crowley, and A.S. Bleiweis: Construction and Characterization of Isogenic Mutants of Streptococcus mutans Deficient in Major Surface Protein Antigen PI (I/II). Infect. Immun. 57:3306-3313 (1989).
66.
Loesche, W.J.: Role of Streptococcus mutans in Human Dental Decay. Microbiol. Rev. 50:353-380 (1986).
67.
Lunsford. R. D. and F.L. Macrina: Molecular Cloning and Characterization of scrB, the Structural Gene for the Streptococcus mutans Phosphoenolpyruvate-Dependent Phosphotransferase System Sucrose-6-Phosphate Hydrolase. J. Bacteriol. 166:426-434 (1986).
68.
Macrina, F.L., M.T. Dertzbaugh, M.C. Halula, E.R. Krah III. and K.R. Jones: Genetic Approaches to the Study of Oral Microflora: A Review. Crit. Rev. Oral Biol. and Med. 1:207-227 (1990).
69.
Macrina. F. L. K.R. Jones, C.-A. Alpert, B.M. Chassy, and S.M. Michalek: Repeated DNA Sequences Involved in Mutations Affecting Transport of Sucrose into Streptococcus mutans V403 via the Phosphoenolpyruvate Phosphotransferase System. Infect. Immun. 59:1535-1543 (1991 ).
70.
Marchman. J. L. G.R. Shah, R.T. Taylor, and P.W. Caufield. Alteration of Aciduricity in Streptococcus mutans by Tn916 Mutagenesis. J. Dent. Res. 69 (Spec. Issue):325 ( 1990).
71.
McCabe, M.M. R. Hamelik, and E. Smith: Purification of Dextran-Binding Protein from Streptococcus mutans. Biochem. Biophys. Res. Commun. 78:273-278 (1977).
72.
Michalek, S.M. and N.K. Childers: Development and Outlook for a Caries Vaccine, Crit. Rev. Oral Biol. Med. 1:37-54 (1990).
73.
Minah, G.E. and W.J. Loesche: Sucrose Metabolism by Prominent Members of the Flora Isolated from Cariogenic and Non-Cariogenic Dental Plaques. Infect. Immun. 17:55-61 (1977).
74.
Mooser. G. and C. Wong: Isolation of a Glucan-Binding Domain of Glucosyltransferase (1,6-a-Glucan Synthase) from Streptococcus sobrinus. Infect. Immun. 56:880-884 (1988).
75.
Munro, C., S.M. Michalek, and F.L. Macrina: Cariogenicity of Streptococcus mutans V403 Glucosyltransferase and Fructosyltransferase Mutants Constructed by Allelic Exchange. Infect. Immun. 59:2316-2323 (1991).
76.
Murchison, H., S. Larrimore, and R. Curtiss III: Isolation and Characterization of Streptococcus mutans Mutants Defective in Adherence and Aggregation. Infect. Immun. 34: 1044-1055 1981.
77.
Newbrun, E.: Sugar and Dental Caries: A Review of Human Studies. Science217:418-423 (1982).
78.
Perry, D., and H.K. Kuramitsu: Genetic Transformation of Streptococcus mutans. Infect. Immun. 32:1295-1297 (1981).
79.
Poy, F., and G.R. Jacobson: Evidence that a Low Affinity Sucrose Phosphotransferase Activity in Streptococcus mutans GS-5 is a High-Affinity Trehalose Uptake System. Infect. Immun. 58:1479-1480 (1990).
80.
Russell, M.W.: Protein Antigens of Streptococcus mutans. In: Molecular Microbiology and Immunobiology of Streptococcus mutans, pp. 51-59. ( S. Hamada, S. M. Michalek. H. Kiyono, L. Menaker, and J. R. McGhee, Eds.) Elsevier Science, Amsterdam (1986 ).
81.
Russell, R.R.B.: Glucan-Binding Proteins of Streptococcus mutans Serotype c. J. Gen. Microbiol. 112:197-201 (1979a).
82.
Russell, R.R.B.: Wall-Associated Protein Antigens of Streptococcus mutans. J. Gen. Microbiol. 114: 109-115 (1979b).
83.
Russell, R.R.B.: Genetic Analysis and Genetic Probes for Oral Bacteria. In: Aspects of Oral Molecular Biology. pp. 57-76. (D. B. Ferguson, Ed.) Krager, Basel (1990).
84.
Russell, R.R.B., and G. Coleman: Immunization of Monkeys (Macaca fascicularis) with Purified Streptococcus mutans Glucosyltransferase. Arch. Oral Biol. 26:23-28 (1981).
85.
Russell, R.R.B., D. Coleman, and G. Dougan: Expression of a Gene for Glucan-Binding Protein from Streptococcus mutans in Escherichia coli. J. Gen. Microbiol. 131:295-299 (1985).
86.
Sandham, H.J., J. Brown, H.I. Phillips, and K.H. Chan: A Preliminary Report on Long-Term Elimination of Detectable Mutans Streptococci in Man. J. Dent. Res. 67:9-14 (1988).
87.
Sato, Y., F. Poy, G.R. Jacobson, and H.K. Kuramitsu: Characterization and Sequence Analysis of the serA Gene Encoding Enzyme IIser of the Streptococcus mutans Phosphoenolpyruvate-Dependent Sucrose Phosphotransferase System. J. Bacteriol. 171:263-271 (1989).
88.
Schachtele. C.F., R.H. Staat, and S.K. Harlander: Dextranases from Oral Bacteria: Inhibition of Water-Insoluble Glucan Production and Adherence to Smooth Surfaces by Streptococcus mutans. Infect. Immun. 12:309-317 (1975).
89.
Schilling, K.M. and W.H. Bowen: Glucans Synthesized in situ in Experimental Salivary Pellicle Function as Specific Binding Sites for Streptococcus mutans. Infect. Immun. 60:284-295 (1992).
90.
Schroeder, V.A., S.M. Michalek, and F.L. Macrina: Biochemical Characterization and Evaluation of Virulence of a Fructosyltransferase-Deficient Mutant of Streptococcus mutans V403. Infect. Immun. 57:3560-3567 (1989).
91.
Shimamura, A., H. Tsumori, and H. Mukasa: Three Kinds of Glucosyltransferases from Streptococcus mutans 6715 (serotype g). FEBS Lett. 157:79-84 (1983).
92.
Slee, A.M., and J.M. Tanzer: Sucrose Transport by Streptococcus mutans. Evidence for Multiple Transport Systems. Biochim. Biophys. Acta692:415-524 (1982).
93.
Smith, D.J., and M.J. Taubman: Immunological Characteristics of Synthetic Peptides Derived from Glucosyltransferase (GTF) Sequences Associated with Function. Cariol. Nineties Abstr. No. 8 (1991).
94.
Sommer, P., T. Bruyere, J.A. Ogier, J.-M. Gamier, J.-M. Jeltsch, and J.-P. Klein: Cloning of the Saliva-Interacting Protein Gene from Streptococcus mutans. J. Bacteriol. 169:5167-5173 (1987 ).
95.
Staat, R.H., S.D. Langley, and R.J. Doyle: Streptococcus mutans Adherence: Presumptive Evidence for Protein-Mediated Attachment Followed by Glucan-Dependent Cellular Accumulation. Infect. Immun., 27:675-681 (1980).
96.
Takahashi, I., N. Okahashi, C. Sasakawa, M. Yoshikawa, S. Hamada, and T. Koga: Homology between Surface Protein Antigen Genes of Streptococcus sobrinus and Streptococcus mutans. FEBS Lett. 249:383-388 (1989).
97.
Tanzer, J.M.: Essential Dependence of Smooth Suface Caries on, and Augmentation of Fissure Caries by, Sucrose and Streptococcus mutans Infection. Infect. Immun. 25:526-531 (1979).
98.
Tanzer, J.M.: Microbiology of Dental Caries. In: Contemporary Oral Microbiology and Immunology, pp. 377-424. (J. Slots and M. A. Taubman, Eds.) Mosby Year Book, St. Louis (1992).
99.
Tanzer, J.M., B.M. Chassy, and M.I. Krichevsky: Sucrose Metabolism by Streptococcus mutans SL-1. Biochim. Biophys. Acta261:379-387 (1972).
100.
Tanzer, J.M. and M.L. Freedman: Genetic Alteration of Streptococcus mutans Virulence. In: Secretory Immunity and Infection, pp. 661-672. (J. R. McGhee, J. Mestecky. and J. L. Babb, Eds.) Plenum Press, New York (1978).
101.
Tanzer, J.M., M.L. Freedman, R.J. Fitzgerald, and R.H. Larson: Diminished Virulence of Glucan-synthesis Defective Mutants of Streptococcus mutans Strains. Infect. Immun. 10:197-203 (1974).
102.
Tanzer, J.M., M.L. Freedman, F.N. Woodiel, R.L. Eifert, and L.A. Rinehimer: Association of Streptococcus mutans Virulence with Synthesis of Intracellular Polysaccharides. In: Proceedings: Microbial Aspects of Dental Caries (a Special Supplement to Microbiology Abstracts), pp. 597-616. (H. M. Stiles, W. J. Loesche, and T. C. O'Brien, Eds.) Information Retrieval, Washington, D.C. (1976).
103.
Tanzer, J.M., A.B. Kurase, and J. Clive: Competitive Displacement of Mutans Streptococci and Inhibition of Tooth Decay by Streptococcus salivarius TOVE-R. Infect. Immun. 48:44-50 (1985).
104.
van der Hoeven, J.S. and A.H. Rogers: Stability of the Resident Microflora and the Bacteriocinogeny of Streptococcus mutans as Factors Affecting its Establishment in Specific Pathogen-Free Rats. Infect. Immun. 23:206-212 (1979).
105.
van Houte, J., J. Russo, and K.S. Prostak: Increased pH-Lowering Ability of Streptococcus mutans Cell Mass Associated with Extracellular Glucan-Rich Matrix Material and the Mechanism Involved. J. Dent. Res. 68:451-459 (1989).
106.
van Houte, J., V.N. Upeslacis, H.V. Jordan, Z. Skobe, and D.B. Green: Role of Sucrose in Colonization of Streptococcus mutans in Conventional Sprague-Dawley Rats. Arch. Oral Biol. 55:202-215 (1976).
107.
Vickerman, M.M., D.B. Clewell, and G.W. Jones: Sucrose-promoted Accumulation of Growing Glucosyltransferase Variants of Streptococcus gorodonii on Hydroxyapatite Surfaces. Infect. Immun. 59:3523-3530 (1991).
108.
Walker, J.E., M.L. Saraste, and N.J. Gay: The unc Operon. Nucleotide Sequence, Regulation, and Structure of ATP-Synthase. Biochim. Biophys. Acta768:164-200 (1984).
109.
Yamashita, Y., W.H. Bowen, and H.K. Kuramitsu: Molecular Analysis of a Streptococcus mutans Strain Exhibiting Polymorphism in the Tandem gtfB and gtfC Genes. Infect. Immun. 60:1618-1624 (1992).
110.
Yamashita, Y., N. Hanada, and T. Takehara: Purification of a Fourth Glucosyltransferase from Streptococcus sobrinus, J. Bacteriol. 171:6265-6270 (1989 ).
