Photoelectrical Responses of Dried Purple Membrane Film in Blue Form and Acid Purple Form

Abstract

The modification of photoelectrical property of dried purple membrane (PM) film by acid treatment and deionization method is reported. By acid treatment and deionization, blue and acid purple form of bacteriorhodopsin, which have been obtained only in suspension, are able to be obtained in dried PM films. Using these films, the photoelectrical responses for blue and acid purple form of bacteriorhodopsin in dried films are measured. The disappearance of the normal photocur rent and appearance of a photocurrent with an opposite polarity is observed for blue and acid purple form of dried PM. The photoelectrical property of bR in dried PM is able to be modified by acid treatment and deionization. These treatments are able to control the polarity of bacteriorhodopsin photoresponse without change of the orientation of the bacteriorhodopsin molecules.

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