Comparison of Immunogold Silver Staining (IGSS) with Two Immunoperoxidase Staining Systems for the Detection of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Antigens in Formalin-Fixed Tissues

Benfield D Harris L Nelson E : 1992, Properties of SIRS virus isolate ATCC VR-2332 in the United States and preliminary characterization of a monoclonal antibody to this virus. Am Assoc Swine Pract Newsl 4:19–21.

Chamlian A Benkoël L Ikoli JF : 1993, Nuclear immunostaining of hepatitis C infected hepatocytes with monoclonal antibodies to C 100-3 nonstructural protein. Comparison of immunogold silver staining with other immunohistochemical methods. Cell Mol Biol 39:243–251.

Halbur PG Andrews JJ Huffman EL : 1994, Development of a streptavidin-biotin immunoperoxidase procedure for the detection of porcine reproductive and respiratory syndrome virus antigen in porcine lung. J Vet Diagn Invest 6:254–257.

Holgate CS Jackson P Cowen PN Bird CC : 1983, Immunogold-silver staining: New method of immunostaining with enhanced sensitivity. J. Histochem Cytochem 31:938–944.

Larochelle R Magar R : 1993, The application of immunogold silver staining (IGSS) for the detection of transmissible gastroenteritis virus in fixed tissues. J Vet Diagn Invest 5:16–20.

Larochelle R Sauvageau R Magar R : 1994, Immunohistochemical detection of swine influenza virus and porcine reproductive and respiratory syndrome virus in porcine proliferative and necrotizing pneumonia cases from Quebec. Can Vet J 35:513–515.

Magar R Larochelle R : 1992, Immunohistochemical detection of porcine rotavirus using immunogold silver staining (IGSS). J Vet Diagn Invest 4:3–7.

Magar R Larochelle R Robinson Y Dubuc C : 1993, Immunohistochemical detection of porcine reproductive and respiratory syndrome virus using colloidal gold. Can J Vet Res 57:300–304.

Magar R Robinson Y Dubuc C Larochelle R : 1995, Isolation and experimental oral transmission in pigs of a porcine reproductive and respiratory syndrome virus isolate. In: Corona and related viruses, advances in experimental and biology series, ed. Talbot PJ Levy GA , pp. 134–144. Plenum Publishing Corporation, New York, NY.

Nelson EA Christopher-Hennings J Drew T : 1993, Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J Clin Microbiol 31:3184–3189.

Zeman D Neiger R Yaeger M : 1993, Laboratory investigation of PRRS virus infection in three swine herds. J Vet Diagn Invest 5:522–528.