Fast and accurate detection of foot-and-mouth disease (FMD) outbreaks is needed to limit spread of the disease by proper vaccination. The use of the polymerase chain reaction (PCR) has revolutionized the way in which viral diseases are diagnosed. Sequence analysis of the amplified VP1 sequence can enable the classification of FMD virus detected in the morbid animal. PCR assays were carried out to identify the virus and its serotype in suspect animals from 2 outbreaks of FMD type O virus. Sequence analysis of the amplified VP1 cDNA showed 78% homology with O1K and over 95% homology between the samples. These findings suggest that the 2 outbreaks were due to infection with the same virus serosubtype.
References
1.
AcharyaRFryEStuartD: 1989, The three-dimensional structure of foot-and-mouth disease virus at 2.9 Å resolution. Nature337:709–716.
2.
BachrachHL: 1977, Foot and mouth disease virus: properties, molecular biology, and immunogenicity. In: Beltsville symposia on agricultural research, ed. Romberger, pp. 3–32. Allanheld, Osmun & Co., Montclair, NJ.
3.
BittleJLHoughtenRAAlexanderH: 1982, Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature298:30–33.
4.
CarrollARRowlandsDJClarkBE: 1984, The complete nucleotide sequence of the RNA for the primary translation product of foot and mouth disease virus. Nucleic Acids Res12:2461–2472.
5.
DopazoJSobrinoFPalmaELDomingoE: 1988, Gene encoding capsid protein VP1 of foot and mouth disease virus: a quasispecies model of molecular evolution. Proc Natl Acad Sci USA85:6811–6815.
6.
FrossSStrebelKBeckEScallerH: 1984, Nucleotide sequence and genomic organization of foot and mouth disease virus. Nucleic Acids Res12:6587–6601.
7.
LaorOTorgersenHYadinHBeckerY: 1992, Detection of FMDV RNA amplified by the polymerase chain reaction (PCR). J Virol Methods36:197–208.
8.
LaorOYadinHChaiDBeckerY: 1991, Detection of foot and mouth disease virus in diagnostic material using the PCR method on viral genomic poly-A isolated with oligo dT on magnetic beads. Isr J Vet Med46:127–133.
9.
LewisSAMorganDOGrubmanMJ: 1991, Expression, processing of foot-and-mouth disease virus capsid structure in heterologous system: induction of a neutralizing antibody response in guinea pigs. J Virol65:6572–6580.
10.
LoganDAbu-GhazalehBlackmore W: 1993, Structure of a major immunologic site on foot-and-mouth disease virus. Nature362:566–568.
11.
MeyerRFBrownCHouseJAMiltorTW: 1991, Rapid and sensitive detection of foot and mouth disease virus in tissues by enzymatic RNA amplification of the polymerase gene. J Virol Methods34:161–172.
12.
RodriguezAMartinez-SalasEDopazoJ: 1992, Primer design for specific diagnosis by PCR of highly variable RNA viruses: typing of foot and mouth disease virus. Virology189:363–367.
13.
SambrookJFritchEFManiatisT: 1989, Molecular cloning; a laboratory manual, 2nd ed.Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
14.
StramYYadinHChaiD: 1993, Characterization of foot and mouth disease virus (FMDV) serotypes using the polymerase chain reaction. Isr J Vet Med48:57–60.
15.
StrohmaierKFranzeRAdamISH: 1982, Location and characterization of the antigenic portion of the FMDV immunizing protein. J Gen Virol59:295–306.
