Porcine reproductive and respiratory syndrome virus (PRRSV) MN-1b strain open reading frame 4 (ORF4) has been cloned, sequenced, and expressed in Escherichia coli. The homologies of nucleotide and amino acid sequences between MN-1b (US isolate) and LV (European isolate) are 69% and 64%, respectively. The data also showed that ORF4 of MN-1b is 36 bases shorter than that of LV. Western blot analysis of expressed recombinant ORF4 protein reacted with 65% (26/40) of PRRSV-infected pig sera tested. These results demonstrated that ORF4 of PRRSV may not be a well-conserved region.
References
1.
AhlRPensaertMRobertsonIB: 1992, Porcine reproductive and respiratory syndrome (PRRS or blue-eared pig disease). Vet Rec130:87–89.
2.
BautistaEMGoyalSMCollinsJE: 1993, Serologic survey for Lelystad and VR-2332 strains of porcine respiratory and reproductive syndrome (PRRS) virus in U.S. swine herds. J Vet Diagn Invest5:612–614.
3.
BenfieldDANelsonECollinsJE: 1992, Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest4:127–133.
4.
CollinsJEBenfieldDAChristiansonWT: 1992, Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the diseases in gnotobiotic pigs. J Vet Diagn Invest4:117–126.
5.
ConzelmannIXVisserNVan WoenselP: 1993, Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology193:329–339.
6.
den BoonJASnijderETChimsideED: 1991, Equine arteritis virus is not a togavirus but belongs to the coronavirus superfamily. J Virol65:2910–2920.
7.
KimHSKwangJYoonIJ: 1993, Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch Virol133:477–83.
8.
KwangJLittledikeETDonisRO: 1992, Recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (BVDV) detects serum antibodies in vaccinated and infected cattle. Vet Microbiol32:281–292.
9.
LaemmliUK: 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685.
10.
MeulenbergJJMHulstMMde MeuerEJ: 1993, Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology192:62–72.
11.
NelsonEAChristopher-HenningsJDrewT: 1993, Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J Clin Microbiol31:3184–3189.
12.
PatonDJBrownIHEdwardsS: 1991, Blue ear disease of pigs. Vet Rec128:617.
13.
PlagemanPGWMoennigV: 1992, Lactate dehydrogenase-elevating virus, equine arterits virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Adv Virus Res41:99–183.
14.
TowbinHStaehelinTGordonJ: 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA76:4350–4354.
15.
WensvoortGde KluyverEPLuijtzeEA,: 1992, Antigenic comparison of Lelystad virus and swine infertility and respiratory syndrome (SIRS) virus. J Vet Diagn Invest4:134–138.
16.
WensvoortGTerpstraCPolJMA: 1991, Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Vet Q13:121–130.
17.
YoonJJooHSChristiansonWT: 1992, An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J Vet Diagn Invest4:144–147.
