Comparison of Particle Concentration Fluorescence Immunoassay to Card and Complement Fixation Tests Using Isolation of Brucella Abortus as the Standard
Free accessResearch articleFirst published online April, 1994
Comparison of Particle Concentration Fluorescence Immunoassay to Card and Complement Fixation Tests Using Isolation of Brucella Abortus as the Standard
Serologic test data and bacteriologic culture results from 816 cattle were evaluated. Brucella abortus (field strain or strain 19) was isolated from 27.3% of the cows. Results of the card test, particle concentration fluorescence immunoassay (PCFIA), and complement fixation (CF) test were compared. Antibody titers were directly associated with B. abortus isolation, with the majority (64.7%) of field strain isolations having a PCFIA value of ≤0.300 and a CF test result of at least 1 + at a 1:160 dilution. The specificity of the CF test was significantly higher than that of the PCFIA (at CF ≥ 1:40 and PCFIA ≥ 0.300).
References
1.
FleissJL: 1981, Statistical methods for rates and proportions, 2nd ed., pp. 13–15.
2.
HuberJDNicolettiP: 1986, Comparison of the results of card, Rivanol, complement fixation, and milk ring tests with the isolation rate of Brucella abortus from cattle. Am J Vet Res47: 1529–1531.
3.
MartinSWMeekAHWillebergP: 1987, Measurement of disease frequency and production. Vet Epidemiol Princ Methods73–75.
4.
MorganWJMcDiarmidA: 1960, The excretion of Brucella abortus in the milk of experimentally infected cattle. Res Vet Sci53–56.
5.
NicolettiPTanyaV: 1993, Comparison of enzyme-labeled immunosorbent assay and particle concentration fluorescence immunoassay with standard serologic methods and bacteriologic culture for detection of Brucella sp.-infected cows in herds with brucellosis. J Am Vet Med Assoc1975–1977.
6.
RohtLHSelwynBJHolguinAHChristensenBL: 1982, Principles of epidemiology, pp. 223–224.
7.
US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services: 1985, Laboratory procedures for isolating, identifying, and typing Brucella. USDA, Washington, DC.
8.
US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services: 1986, Microtitration complement fixation techniques.USDA, Washington, DC.
9.
US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Standard agglutination test procedures for the diagnosis of brucellosis. Diagnostic reagents manual 65D. USDA, Washington, DC.
10.
US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Supplemental test procedures for the diagnosis of brucellosis. Diagnostic reagents manual 65E. USDA, Washington, DC.
11.
WrightPFNielsenKH: 1990, Current and future serological methods. In: Advances in brucellosis research, ed. AdamsLG, pp. 305–320. Texas A&M University Press, College Station, TX.
