Free accessResearch articleFirst published online 1993-1
Comparison of a Monoclonal Antibody Capture ELISA (MACELISA) to Indirect ELISA and Virus Neutralization Test for the Serodiagnosis of Transmissible Gastroenteritis Virus
An enzyme-linked immunosorbent assay (ELISA) in which the antigen is captured to the plate by monoclonal antibodies (MACELISA) was developed for the detection of antibodies to transmissible gastroenteritis virus (TGEV). The viral antigen was semipurified from TGEV-infected cells by simple ultracentrifugation. MACELISA results with 258 field sera were compared with those of a standard indirect ELISA and with the virus neutralization test (VNT). Sensitivity, specificity, and kappa values of MACELISA indicated a strong correlation with VNT results, whereas an indirect ELISA was less sensitive and much less specific than VNT. The serologic response of 4 pigs orally inoculated and intraperitoneally boostered with TGEV was compared using the 3 tests. Its sensitivity, specificity, and ability to use unpurified antigen make the MACELISA the advisable first step in TGEV serodiagnosis.
References
1.
BanJGiecicovaEOrlickOAltanerC: 1990, Use of monoclonal antibodies in an ELISA for the diagnosis of bovine leukemia virus infection. J Virol Methods30:79–88.
2.
BernardSLaudeHLantierI: 1986, Detection du coronavirus de la GET et des anticorps specifiques par une technique ELISA mixte mettant en ouvre anticorps monoclonaux et policlonaux. J Rech Porcine Fr18:363–370.
3.
BohlEH: 1989, Transmissible gastroenteritis (classical enteric variant). In: Virus infections of porcines, ed. PensaertM.Elsevier Science B.V., Amsterdam, The Netherlands.
4.
BrownIHCartwrightSF: 1986, New porcine coronavirus?. Vet Rec119:282–283.
5.
BrownIHPatonDJ: 1991, Serological studies of transmissible gastro-enteritis in Great Britain using a competitive ELISA. Vet Rec128:500–503.
6.
CallebautPCorreaIPensaertM: 1988, Antigenic differentiation between transmissible gastroenteritis virus of swine and a related porcine respiratory coronavirus. J Gen Virol69: 1725–1730.
7.
CallebautPPensaertMBHooybergsJ: 1988, A competitive-inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus or with the TGEV-related porcine respiratory coronavirus. Vet Microbiol20: 9–19.
8.
CorreaIGebauerFBullidoM: 1990, Localization of the antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus. J Gen Virol71:271–279.
9.
CoxEHooybergsJPensaertMB: 1990, Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus. Res Vet Sci48: 165–169.
10.
CoxEPensaertMBCallebautPVan DeunK: 1990, Intestinal replication of a porcine respiratory coronavirus closely related antigenically to the enteric transmissible gastroenteritis virus. Vet Microbiol23:237–243.
11.
DoyleLPHutchingsLM: 1946, A transmissible gastroenteritis in pigs. J Am Vet Med Assoc108:257–259.
12.
FrankenaKNoordhuizenJWillebergP: 1990, EPI-SCOPE: computer programs in veterinary epidemiology. Vet Rec126:573–576.
13.
GarwesDJSterwartFCartwrightSFBrownI: 1988, Differentiation of porcine coronavirus from transmissible gastroenteritis virus. Vet Rec122:86–87.
14.
HofmannMWylerR: 1990, Enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea coronavirus antibodies in swine sera. Vet Microbiol21:263–273.
15.
HohdatsuTEiguchiYIdeS: 1987, Evaluation of an enzyme-linked immunosorbent assay for the detection of transmissible gastroenteritis virus antibodies. Vet Microbiol13:93–97.
16.
JestinALe ForbanYVannierP: 1987, Un nouveau coronavirus porcin. Etudes séro-épidémiologiques retrospectives dans les élévages de Bretagne. Rec Méd Vét163:567–571.
MartinSMeekAWillebergP: 1987, Veterinary epidemiology. Principles and methods. Iowa State University Press, Ames, IA.
19.
NelsonLKellingC: 1984, Enzyme-linked immunosorbent assay for detection of transmissible gastroenteritis virus antibody in swine sera. Am J Vet Res45:1654–1657.
20.
O'TooleDBrownIBridgesACartwrightSF: 1989, Pathogenicity of experimental infection with “pneumotropic” porcine coronavirus. Res Vet Sci47:23–29.
21.
PatonDJBrownIHVazE: 1991, An ELISA for the detection of serum antibodies to both transmissible gastroenteritis virus and porcine respiratory coronavirus. Br Vet J147:370–372.
22.
PensaertM: 1989, Transmissible gastroenteritis virus (respiratory variant). In: Virus infections of porcines, ed. PensaertM.Elsevier Science B.V., Amsterdam, The Netherlands.
23.
PensaertMBCallebautPVergoteJ: 1986, Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet Q8:257–261.
24.
RasschaertDDuarteMLaudeH: 1990, Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. J Gen Virol71:2599–2607.
25.
ReynoldsDJ: 1982, Application of ELISA to the study of viruses. Development and application of ELISA for veterinary diagnosis and research on enteric and other viruses. In: The ELISA: enzyme-linked immunosorbent assay in veterinary research and diagnosis, ed. WardleyRCCrowtherJR. Martinus Nijhoff, The Hague, The Netherlands.
26.
RukhadzeGAlipertTSergeevV: 1989, Isolation of peplomer glycoprotein E2 of transmissible gastroenteritis virus and application in enzyme-linked immunosorbent assay. J Clin Microbiol27: 1754–1758.
27.
SanchezCMJimenezGLaviadaMD: 1990, Antigenic homology among coronaviruses related to transmissible gastroenteritis virus. Virology174:410–417.
28.
Sanchez-VizcainoJMCambraM: 1987, In: Technicas inmunoenzimaticas ELISA en patología animal y vegetal. Publicación técnica No. 7. INIA-OIE.
29.
TomaBBenetJ: 1976, Technique de recherche sur microplaques des anticorps neutralisant le virus de la gastroentérite transmissible du porc. Rec Méd Vét152:565–568.
30.
VannierP: 1990, Disorders induced by the experimental infection of pigs with the porcine respiratory coronavirus (P.R.C.V.). J Vet Med Ser B37: 177–180.
31.
WesleyRDWoodsRDHillHTBiwerJD: 1990, Evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the United States. J Vet Diagn Invest2:312–317.
