Sage Journals: Discover world-class research

Abstract

A plasmid, pG3BS, containing a cDNA clone from the 5′ coding region of the peplomer glycoprotein gene appears to be specific for enteric transmissible gastroenteritis virus (TGEV) strains and for live-attenuated TGEV vaccines. This cDNA probe is used to differentiate porcine respiratory coronavirus (PRCV) isolates from TGEV field and vaccine strains by a slot blot hybridization assay. Probe pG3BS also hybridizes to canine coronavirus (CCV) RNA but does not hybridize to antigenically related feline infectious peritonitis virus (FIPV) RNA. The RNAs of 13 enteric TGEV isolates from the United States, Japan, and England, 4 US-licensed live-attenuated TGEV vaccines, and antigenically closely related CCV were detected by pG3BS. The RNAs of FIPV and 3 US isolates of PRCV did not react with pG3BS but were detected by a TGEV-derived plasmid, pRP3. Pigs infected with either PRCV or TGEV test serologically positive for TGEV antibody by the serum neutralization test. Characterization of the virus circulating in a swine herd by the pG3BS probe will differentiate between an enteric TGEV and a respiratory PRCV infection.

References

Callebaut

Pensaert

Hooyberghs

: 1989, A competitive inhibition ELISA for the differentiation of serum antibodies from pigs infected with transmissible gastroenteritis virus (TGEV) or with the TGEV-related porcine respiratory coronavirus. Vet Microbiol 20:9–19.

Church

Gilbert

: 1984, Genome sequencing. Proc Natl Acad Sci USA 81:1991–1995.

Cox

Hooyberghs

Pensaert

: 1990, Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus. Res Vet Sci 48: 165–169.

Doyle

Hutchings

: 1946, A transmissible gastroenteritis in pigs. J Am Vet Med Assoc 108:257–259.

Gallagher

Parker

Buchmeier

: 1990, Neutralization-resistant variants of a neurotropic coronavirus are generated by deletions within the amino-terminal half of the spike glycoprotein. J Virol 64:731–741.

Garwes

Stewart

Cartwright

Brown

: 1988, Differentiation of porcine coronavirus from transmissible gastroenteritis virus. Vet Ret 122:86–87.

Jacobs

de Groot

van der Zeijst

BAM

: 1987, The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus (TGEV): Comparison with the sequence of the peplomer protein of feline infectious peritonitis virus (FIPV). Virus Res 8:363–371.

Kemeny

: 1975, Antibody response in pigs inoculated with transmissible gastroenteritis virus and cross reactions among ten isolates. Can J Comp Med 40:209–214.

Maniatis

Fritsch

Sambrook

: 1982, Molecular cloning (a laboratory manual). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

10.

Myint

Siddell

Tyrrell

: 1989, Detection of human coronavirus 229E in nasal washings using RNA: RNA hybridization. J Med Virol 29:70–73.

11.

Parker

Gallagher

Buchmeier

: 1989, Sequence analysis reveals extensive polymorphism and evidence of deletions within the E2 glycoprotein gene of several strains of murine hepatitis virus. Virology 173:664–673.

12.

Pensaert

Callebaut

Vergote

: 1986, Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet Q 8:257–261.

13.

Underdahl

Mebus

Stair

Twiehaus

: 1972, The effect of cytopathogenic transmissible gastroenteritis-like viruses and/or Escherichia coli on germfree pigs. Can Vet J 13:9–16.

14.

Wesley

: 1991, Nucleotide sequence of the E2-peplomer protein gene and partial nucleotide sequence of the upstream polymerase gene of transmissible gastroenteritis virus (Miller strain). In: Coronaviruses and Their Diseases, ed. Cavanagh

Brown

TDK

. pp. 301–306. Plenum Publishing Corp., New York, NY.

15.

Wesley

Woods

Cheung

: 1990, Genetic basis for the pathogenesis of transmissible gastroenteritis virus. J Virol 64:4761–4766.

16.

Wesley

Woods

Hill

Biwer

: 1990, Evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the United States. J Vet Diagn Invest 2: 312–317.

17.

Woods

: 1978, Small plaque variant transmissible gastroenteritis virus. J Am Vet Med Assoc 173:643–647.

18.

Woods

: 1982, Studies of enteric coronaviruses in a feline cell line. Vet Microbiol 7:427–435.

19.

Woods

Wesley

: 1988, Cultivation techniques for animal coronaviruses: Emphasis on feline infectious peritonitis virus, canine coronavirus, transmissible gastroenteritis virus, and porcine hemagglutinating encephalomyelitis virus. J Tissue Cult Methods 11:95–100.

Differentiation between Transmissible Gastroenteritis Virus and Porcine Respiratory Coronavirus Using a cDNA Probe

Abstract

References