An enteric syndrome of turkey poults, characterized by enteritis, crop mycosis, intestinal changes (pale, thin-walled ballooning with watery contents), and rickets, occurred during 1988 in 74 turkey flocks from different farms belonging to 9 California turkey growers. The flocks ranged in size from 9,000 to 120,000 birds. Pools of intestine sections from 618 birds, representing 78 field cases, were examined. Histopathological examination of the intestines showed a mild to severe atrophy with a reduced depth of crypts, which was more prominent in the distal part of the small intestine. Viral isolation attempts with primary cell cultures of chicken embryo kidney cells were negative. Examination by electron microscopy of negatively stained intestinal specimens revealed the presence of Reoviridae particles of 58.8 to 80 nm in diameter. Enzyme-linked immunosorbent assay results on the intestinal pools for mammalian and group A avian rotaviruses were negative. A statistically significant relationship was found for the presence of Reoviridae particles in the intestines of 10-21-day-old birds. Of the 7 most common pathological conditions analyzed, 2, rickets and intestinal changes (thin-walled ballooning intestine with watery contents), showed a statistically significant association with the presence of Reoviridae particles.
References
1.
DanielWN: 1983, Chi-square distribution: Test of homogeneity. Biostatistics: A foundation for analysis in the health sciences, 3rd ed., pp. 376–380. Wiley and Sons, Inc., New York.
2.
DecaessteckerMCharlierGMeulemansG: 1988, Epidemiological study of enteric viruses in broiler chickens: Comparison of tissue culture and direct electron microscopy. Avian Pathol17:477–486.
3.
DhillonASSchabergDM: 1987, Salmonella contamination in commercial poultry. Proc Western Poult Dis Conf37:118–120.
4.
DoaneFWAndersonN: 1987, Reoviridae. Electron microscopy in diagnostic virology, 1st ed., pp. 108–115. Cambridge University Press, Cambridge, London.
5.
FarrellJAHarveyJDBellamyAR: 1974, Biophysical studies of reovirus type 3. Virology62: 145–153.
GiambroneJJ: 1985, Vaccinating to control reovirus associated diseases. Poult Digest March:96–100.
8.
GoodwinMA: 1987, Cryptosporidiosis in chickens and turkeys: A review. Proc Western Poult Dis Conf37: 142–146.
9.
GoodwinMALatimerKenneth SL: 1984, Quantitation of intestinal D-xylose absorption in normal and reovirus inoculated turkeys. Avian Dis28:959–967.
10.
GoodwinMASteffensWLRussellIDBrownJ: 1988, Diarrhea associated with intestinal cryptosporidiosis in turkeys. Avian Dis32:63–67.
11.
GouveaRSchnitzerPJ: 1982, Pathogenicity of avian reoviruses: Examination of six isolates and a vaccine strain. Infect Immun38:731–738.
12.
GrahamDL: 1987, Characterization of a reo-like virus and its isolation from and pathogenicity for parrots. Avian Dis31:419–419.
13.
GuyJSLevyMGLeyDH: 1987, Experimental reproduction of enteritis in bobwhite quail (Colinus virginianus) with Cryptosporidium and reovirus. Avian Dis31:713–722.
14.
HarveyJDFarrellJABellamyAR: 1974, Biophysical studies of reovirus type 3. II. Properties of the hydrated particle. Virology62:154–160.
15.
HuangDDNugentMARosenbergerJKSchnitzerTJ: 1987, Association of avian reovirus M and S genes with viral behavior in vivo. II Viral pathogenicity. Avian Dis31:446–454.
16.
HyeronymusDRKVillegasPKlevenSH: 1983, Identification and serological differentiation of several reovirus strains isolated from chickens with suspected malabsorption syndrome. Avian Dis27:246–254.
17.
KibengeFSBDhillonAS: 1987, Rapid passage of avian reovirus in one-day-old chicks, clinical and virological findings. Avian Pathol16:213–225.
18.
KibengeFSBDhillonAS: 1987, A comparison of the pathogenicity of four avian reoviruses in chickens. Avian Dis31:39–42.
19.
Le MinorL: 1984, Genus III. Salmonella. In: Bergey's manual of systemic bacteriology, ed. KriegN, vol. 1, pp. 427–458. Williams & Wilkins, Baltimore, MD.
20.
LeyDHLevyMGHunterL: 1988, Crytosporidia positive rates of avian necropsy accessions determined by examination of Auramine O-stained fecal smears. Avian Dis32: 108–113.
21.
McNultyMSCurranWLMcFerranJB: 1980, Detection of astroviruses in turkey faeces by direct electron microscopy. Vet Rec28:561.
22.
McNultyMSCurranWLToddDMcFerranJB: 1979, Detection of viruses in avian faeces by direct electron microscopy. Avian Pathol8:239–247.
23.
McNultyMSToddDAllanGM: 1984, Epidemiology of rotavirus infection in broiler chickens: Recognition of four serotypes. Arch Virol81:113–121.
24.
MerrillRanck FHoerrFJ: 1987, Cryptosporidia in the respiratory tract of turkeys. Avian Dis31:389–391.
25.
NersessianBNGoodwinMAKlevenSHPestiD: 1985, Studies on orthoreoviruses isolated from young turkeys. I. Isolation and characterization. Avian Dis29:755–767.
26.
NersessianBNGoodwinMAPageRK: 1986, Studies on orthoreoviruses isolated from young turkeys. III. Pathogenic effects in chicken embryos, chicks, poults and suckling mice. Avian Dis30:585–592.
27.
OlsonND: 1984, Reovirus infections. In: Diseases of poultry, ed. HofstadMSBarnesJHCalneckBW, 8th ed., pp. 560–566. Iowa State University Press, Ames, IA.
28.
PalmerELMartinML: 1988, Reoviridal. Electron microscopy in viral diagnosis, pp. 75–82. CRC Press Inc., Boca Raton, FL.
29.
ReynoldsDLSaifYM: 1986, Astrovirus: A cause of an emetic disease in turkey poults. Avian Dis30:728–735.
30.
ReynoldsDLSaifYMTheilKW: 1987, A survey of enteric viruses of turkey poults. Avian Dis31:89–98.
31.
ReynoldsDLSaifYMTheilKW: 1987, Enteric viral infections of turkey poults: Incidence of infection. Avian Dis31:272–276.
32.
ReynoldsDLTheilKWSaifYM: 1987, Demonstration of rotavirus and rotavirus-like virus in the intestinal contents of diarrheic pheasant chicks. Avian Dis31:376–379.
33.
RuffMDRosenbergerJK: 1985, Interaction of low pathogenicity reoviruses and low levels of infection with several coccidial species. Avian Dis29:1057–1065.
34.
SaifLJ: 1989, Immunodiagnosis of enteric viral infections: Applications of electron microscopy and monoclonal antibodies. Proc Western Poultry Dis Conf38:232–233.
35.
SaifLJSaifYMTheilKW: 1985, Enteric viruses in diarrheic turkey poults. Avian Dis29:798–811.
36.
TakaseKNonakaFYamamotoMYamadaS: 1987, Serologic and pathogenic studies on avian reoviruses isolated in Japan. Avian Dis31:464–469.
37.
TangKNFletcherOJVillegasP: 1987, Comparative study of the pathogenicity of avian reoviruses. Avian Dis1:577–583.
38.
TheilKW: 1987, A modified genome electropherotyping procedure for detecting turkey rotaviruses in small volumes of intestinal contents. Avian Dis31:899–903.
39.
TheilKWReynoldsDLSaifYM: 1986, Comparison of immune electron microscopy and genome electropherotyping techniques for detection of turkey rotaviruses and rotavirus-like viruses in intestinal contents. J Clin Microbiol23:695–699.
40.
Van der HeideLLuttickenDHorzinckM: 1981, Isolation of avian reoviruses as a possible agent of osteoporosis (“brittle bone disease” “femoral head necrosis”) in broiler chickens. Avian Dis25:847–856.
