Serologic Examination of Aborted Ovine and Bovine Fetal Fluids for the Diagnosis of Border Disease,Bluetongue,Bovine Viral Diarrhea,and Leptospiral Infections
Free accessResearch articleFirst published online April, 1989
Serologic Examination of Aborted Ovine and Bovine Fetal Fluids for the Diagnosis of Border Disease,Bluetongue,Bovine Viral Diarrhea,and Leptospiral Infections
Fetal serum from most of 994 bovine and 553 ovine aborted fetuses was tested serologically for antibodies to border disease (BD), bovine viral diarrhea (BVD), and bluetongue (BT) viruses, and to Leptospira sp., and the results were compared with the results of isolation procedures, fluorescent antibody tests (FAT), and histologic examinations of the same fetuses. Antibodies to BT virus were not found in any of the 994 bovine and 553 ovine fetuses. Antibody titers to BVD virus were present in 39 of 966 bovine fetuses tested, and BVD virus was detected in 4 of the 39. Four of 74 fetuses in which the BVD virus was detected by FAT or isolation had titers to BVD virus. Microagglutination (MAT) titers to 1 or more of 5 serovars of leptospires were present in 52 of 773 bovine fetal sera tested. Leptospires were not detected by FAT in any bovine fetuses that had leptospiral antibody titers. Leptospires were detected by FAT in 15 aborted calves, and none of these had MAT titers. Antibody titers to BD virus were present in 80 of 486 fetal lamb sera tested, and the virus was detected by FAT or isolation in 3 of the 80 fetuses. Border disease virus was detected in 14 of 486 fetal lambs tested. Twelve of the 14 were tested serologically and 3 had titers to BD virus. Leptospiral antibody titers were present in 27 of 326 ovine fetal sera tested. Leptospires were not detected in any of the 326 ovine fetuses tested by FAT.
References
1.
AndersonCASawyerMHigginsRJ: 1987, Experimentally induced ovine border disease: Extensive hypomyelination with minimal viral antigen in neonatal spinal cord. Am J Vet Res48:499–503.
2.
BraunRKOsburnBIKendrickJW: 1973, Immunologic response of bovine fetus to bovine viral diarrhea virus. Am J Vet Res34:1127–1132.
3.
BuxtonDFinlaysonJ: 1986, Experimental infection of pregnant sheep with Toxoplasma gondii: Pathological and immunological observations on the placenta and foetus. J Comp Pathol96:319–333.
4.
CarbreyEABrownLNChowTL: 1971, Recommended standard laboratory techniques for diagnosing infectious bovine rhinotracheitis, bovine virus diarrhea, and shipping fever (para-influenza-3). Proc Annu Meet US Anim Health Assoc57:629–648.
5.
CarterGR: 1984, Diagnostic procedures in veterinary bacteriology and mycology, 4th ed.Charles C Thomas, Springfield, IL.
6.
CasaroAPEKendrickJWKennedyPC: 1971, Response of the bovine fetus to bovine viral diarrhea-mucosal disease virus. Am J Vet Res32:1543–1561.
7.
ColeJREllinghausenHCRubinHL: 1979, Laboratory diagnosis of leptospirosis of domestic animals. Proc Annu Meet US Anim Health Assoc83: 189–199.
8.
DesmontsGRemingtonJS: 1980, Direct agglutination test for diagnosis of toxoplasma infection: Method for increasing sensitivity and specificity. J Clin Microbial11:562–568.
9.
DubeyJPHughesHPALillehojHS: 1987, Placental transfer of specific antibodies during ovine toxoplasmosis. Am J Vet Res48:474–476.
10.
FaheyKJBrandonMR: 1978, Synthesis of immunoglobulin by normal and antigenically stimulated fetal sheep. Res Vet Sci25:218–224.
11.
FennestadKLBorg-PetersenC: 1957, Leptospira antibody production by bovine foetuses. Nature180:1210–1211.
12.
FrenchELHoreDESnowdonWA: 1974, Infection of pregnant ewes with mucosal disease virus of ovine origin. Aust Vet J50:45–54.
13.
GardGPAcklandHMPlantJW: 1976, A mucosal disease virus as a cause of abortion, hairy birth coat, and unthriftiness in sheep: 2. Observations on lambs surviving longer than seven days. Aust Vet J52:64–68.
14.
GardinerACZakarianBBarlowRM: 1980, Periarteritis in experimental border disease of sheep: III. Immunopathological observations. J Comp Pathol90:469–474.
15.
GibsonCDZemjanisR: 1973, Immune response of the bovine fetus to several antigens. Am J Vet Res34:1277–1280.
HageageGJHarringtonBJ: 1984, Use of calcofluor white in clinical mycology. Lab Med15:109–112.
18.
HarknessJWLamontPH: 1974, Laboratory diagnosis of mucosal disease. Vet Rec96:17–18.
19.
JerrettIVMcOristSWaddingtonJ: 1984, Diagnostic studies of the fetus, placenta, and maternal blood from 265 bovine abortions. Cornell Vet74:8–20.
20.
JochimMMLuedkeAJChowTL: 1974, Bluetongue in cattle: Immunogenic and clinical responses in calves inoculated in utero and after birth. Am J Vet Res35:517–522.
21.
JochimMMPearsonJE: 1979, Protocol for the immunodiffusion test for bluetongue. Proc Annu Meet Am Assoc Vet Lab Diagn22:463–471.
22.
KahrsRFScottFWHillmenRB: 1972, An appraisal of fetal serology for the diagnosis of bovine abortion and congenital defects. Proc Annu Meet US Anim Health Assoc76:588–594.
23.
KendrickJW: 1971, Bovine viral diarrhea-mucosal disease virus infection in pregnant cows. Am J Vet Res32:533–544.
24.
KendrickJKirkbrideCA: 1983, Bovine abortion caused by the bovine viral diarrhea virus. In: Laboratory diagnosis of abortion in food animals, ed. KirkbrideCA, pp. 133–139. Am Assoc Vet Lab Diagn, Tucson, AZ.
25.
KirkbrideCABicknellEJReedDE: 1973, A diagnostic survey of bovine abortion and stillbirth in the northern plains states. J Am Vet Med Assoc162:556–560.
26.
KirkbrideCAHalleyL: 1982, Immunofluorescent and ELISA techniques as aids in diagnosing bovine leptospirosis. Proc Annu Meet Am Assoc Vet Lab Diagn25:345–356.
27.
LangfordEV: 1976, Laboratory procedures. In: Laboratory diagnosis of mycoplasmosis in food animals, ed. StalheimOHV. Am Assoc Vet Lab Diagn, Tucson, AZ.
28.
Leslie-SteenPKirkbrideCA: 1983, Comparison of diagnostic methods for in utero parvovirus infection of swine fetuses. Proc Int Symp Vet Lab Diagn3:111–118.
29.
LeudkeAJJochimMMBrowneJG: 1970, Observation on latent bluetongue virus infection in cattle. J Am Vet Med Assoc156:1871–1879.
30.
MacLachlanNJSchoreCEOsbumBI: 1984, Antiviral responses of bluetongue virus-inoculated bovine fetuses and their dams. Am J Vet Res45:1469–1473.
31.
MengelingWL: 1983, Maternal reproductive failure caused by porcine parvovirus. In: Laboratory diagnosis of abortion in food animals, ed. KirkbrideCA, pp. 140–144. Am Assoc Vet Lab Diagn, Tucson, AZ.
32.
MillerRBQuinnPJ: 1974, Observations on abortions in cattle: A comparison of pathological, microbiological, and immunological findings in aborted foetuses and foetuses collected at abattoirs. Can J Comp Med39:270–290.
33.
MillerRBRuhnkeHLPalmerNC: 1983, Bovine abortion caused by Ureaplasma diversum. In: Laboratory diagnosis of abortion in food animals, ed. KirkbrideCA, pp. 27–33. Am Assoc Vet Lab Diagn, Tucson, AZ.
34.
MillerRBWilkieBN: 1979, The indirect fluorescent antibody technique as a method for detecting antibodies in aborted fetuses. Can J Comp Med43:255–261.
35.
MundayBLDubeyJP: 1986, Serology of experimental toxoplasmosis in pregnant ewes and their foetuses. Aust Vet J63: 353–354.
36.
NicolettiP: 1983, Bovine abortion caused by Brucella sp. In: Laboratory diagnosis of abortion in food animals, ed. KirkbrideCA, pp. 20–23. Am Assoc Vet Lab Diagn, Tucson, AZ.
37.
OsbumBI: 1974, Animal models for the study of immunologic modulation of fetal infectious disease processes. In: The immune system and infectious diseases. 4th Int Convocation Immunol, Buffalo, NY, ed. NaterEMilgromF, pp. 25–32. S. Karger, Basel, Switzerland.
38.
OsbumBIClarkeGLStewardWC: 1973, Border disease-like syndrome in lambs: Antibodies to hog cholera and bovine viral diarrhea viruses. J Am Vet Med Assoc163: 1165–1176.
39.
OsbumBIJohnsonRTSilversteinAM: 1971, Experimental viral-induced congenital encephalopathies: II. The pathogenesis of bluetongue vaccine virus infection in fetal lambs. Lab Invest25:206–210.
40.
OsbumBIStabenfeldtGHArdansAA: 1974, Perinatal immunity in calves. J Am Vet Assoc164:295–298.
41.
PlantJWAclandHMGardGP: 1976, A mucosal disease virus as a cause of abortion, hairy birth coat, and unthriftiness in sheep. Aust Vet J52:57–63.
42.
PoitrasBJMillerRBWilkieBN: 1986, The maternal to fetal transfer of immunoglobulins associated with placental lesions in sheep. Can J Vet Res50:68–73.
43.
ReedDEBicknellEJLarsonGA: 1971, Infectious bovine rhinotracheitis virus induced abortion: Rapid diagnosis by fluorescent antibody technique. Am J Vet Res32:1423–1426.
44.
ReggiardoCKaeberleML: 1981, Detection of bacteremia in cattle inoculated with bovine viral diarrhea virus. Am J Vet Res42:218–221.
45.
RossiCRKieselGKHubbertWT: 1976, Immunoglobulin concentrations and bovine enterovirus inhibitors in fetal bovine fluids. Cornell Vet66:381–386.
46.
RuthGRKirkbrideCALangpapTJ: 1974, Fetal serology as an aid to diagnosis of bovine abortion. Proc Annu Meet Am Assoc Vet Lab Diagn7:9–17.
47.
SawyerMOsbumBIKnightHD: 1973, A quantitative serologic assay for diagnosing congenital infections of cattle. Am J Vet Res34:1281–1284.
48.
SawyerMMSchoreCEMenziesPI: 1986, Border disease in a flock of sheep: Epidemiologic, laboratory, and clinical findings. J Am Vet Med Assoc198:61–65.
49.
SeefeldtSLKirkbrideCA: 1985, Comparison of an enzyme-linked immunosorbent assay and a direct agglutination test for detecting ovine fetal antibodies to Toxoplasma gondii. Proc Annu Meet Am Assoc Vet Lab Diagn28:297–304.
50.
SilversteinAMParshallCJ: 1966, Immunologic maturation in utero: Kinetics of the primary antibody response in the fetal lamb. Science154: 1675–1677.
51.
SilversteinAMThorbeckeGJKraneKL: 1963, Fetal response to antigenic stimulus: III. Gamma globulin production in normal and stimulated fetal lambs. J Immunol91:384–395.
52.
SmithiesLKModdermanE: 1975, BVD virus in commercial fetal calf serum and “normal” and aborted fetuses. Proc Annu Meet Am Assoc Vet Lab Diagn18:113–119.
53.
TerpstraC: 1981, Border disease: Virus persistence, antibody response, and transmission studies. Res Vet Sci30:185–191.
54.
ThomasFCRandallGCBMyersDJ: 1986, Attempts to establish congenital bluetongue virus infections in calves. Can J Vet Res50:280–281.
55.
ThompsonSWHuntRD: 1966, Selected histochemical and histopathologic methods. Charles C Thomas, Springfield, IL.
56.
TokohisaSInabaYSatoK: 1981, Inhibitors of bovine parvovirus, coronavirus, and rotavirus in precolostral and fetal bovine sera. Vet Microbial6:143–155.
