The antimicrobial susceptibility of 73 Actinobacillus (Haemophilus) pleuropneumoniae isolates from swine in Missouri was determined with a microdilution minimal inhibitory concentration test system. Serotyping was accomplished by means of co-agglutination. Serotype 1 (39/73) and serotype 5 (30/73) were commonly found, whereas serotype 7 (4/73) was infrequently encountered. Most isolates (MIC,) were found susceptible to ampicillin (amoxicillin), cephalothin, penicillin, erythromycin, gentamicin, and kanamycin. Marked resistance was found with oxytetracycline, tylosin, and sulfadimethoxine. The data indicate that use of ampicillin (amoxicillin) or penicillin may correlate well with the favorable outcome of treatment.
References
1.
AcarJFGoldsteinFW: 1986, Disk susceptibility test. In: Antibiotics in laboratory medicine, ed. LorianV, 2nd ed., pp. 27–63. Williams & Wilkins, Baltimore, MD.
2.
BauerAWKirbyWSherrisJC: 1966, Antibiotic susceptibility testing by standardized single disk method. Am J Clin Pathol45:493–496.
3.
CarterGR: 1979, Diagnostic procedures in veterinary bacteriology and mycology, 3rd ed., pp. 113–118. Charles C Thomas, Springfield, IL.
4.
FalesWHBergJNMorehouseLG: 1986, Use and comparison of minimal inhibitory concentration and disk diffusion antimicrobial susceptibility testing with bovine isolates of Pasteurella hemolytica type 1 and Pasteurella multocida recovered from Missouri cattle with bovine respiratory disease complex. Proc Annu Meet Am Assoc Vet Lab Diagn29: 1–8.
5.
FalesWHBurrowsGW: 1983, A new in vitro susceptibility testing system for veterinary medical diagnostic laboratories using a commercially prepared stabilized dried antimicrobial minimal inhibitory concentration system. Proc 3rd Int Symp Vet Lab Diagn1:269–275.
6.
FalesWHMorehouseLGKintnerLD: 1982, Prevalence of Haemophilus pleuropneumoniae in Missouri swine. Proc Annu Meet Am Assoc Vet Lab Diagn25:251–260.
7.
HenryS: 1982, Haemophilus pleuropneumoniae in swine. Proc 58th Annu Conf for Veterinarians, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO.
8.
MittalKRHigginsRLariviereS: 1983, Identification and serotyping of Haemophilus pleuropneumoniae by co-agglutination. J Clin Microbial18:1351–1354.
9.
OlanderHJ: 1963, A septicemic disease of swine and its causative agent Haemophilus parahemolyticus. PhD Dissertation, University of California, Davis, CA.
10.
PohlSBertschingerHUFrederiksenWMannheimW: 1983, Transfer of Haemophilus pleuropneumoniae and Pasteurella haemolytica like organisms causing porcine necrotic pleuro-pneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae comb. nov.) on the bases of phenotypic and deoxyribonucleic acid relatedness. Int J Syst Bacterial33: 510–514.
11.
PrescottJFBaggotJD: 1988, Antimicrobial therapy in veterinary medicine, pp. 9–16. Blackwell Scientific Publications, Boston, MA.
