Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1,Bovine Herpesvirus 2,and Bovine Herpesvirus 4 Infections
Free accessResearch articleFirst published online July, 2002
Development of a Polymerase Chain Reaction and Restriction Typing Assay for the Diagnosis of Bovine Herpesvirus 1,Bovine Herpesvirus 2,and Bovine Herpesvirus 4 Infections
A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections. The specificity, sensitivity, and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.
References
1.
BrakeFStuddertMJ: 1985, Molecular epidemiology and pathogenesis of ruminant herpesviruses including bovine, buffalo and caprine herpesviruses 1 and bovine encephalitis herpesvirusAust Vet J62:331–334
2.
BoernerBWeigeltWBuhkHJ: 1999, A sensitive and specific PCR/Southern blot assay for detection of bovine herpesvirus 4 in calves infected experimentallyJ Virol Methods83:169–180
3.
BulachDMStuddertMJ: 1990, Comparative genome mapping of bovine encephalitis herpesvirus, bovine herpesvirus 1, and buffalo herpesvirusArch Virol113:17–34
4.
CandidoABontempoEDResendeM: 2000, Polymerase chain reaction amplification of genomic fragments of bovine herpesvirus-1Mem Inst Oswaldo Cruz95:225–226
5.
CastrucciGFrigeriFFerrariM: 1987, A study of the pathogenesis of bovid herpesvirus 4 in calvesZentralbl Veterinärmed34:473–479
CheeMSBankierATBeckS: 1990, Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169Curr Top Microbiol Immunol154:125–169
8.
ChenCMHuCLWangCH: 1999, Gender determination in single bovine blastomeres by polymerase chain reaction amplification of sex-specific polymorphic fragments in the amelogenin geneMol Reprod Dev54:209–214
9.
CzaplickiGThiryE: 1998, An association exists between bovine herpesvirus-4 seropositivity and abortion in cowsPrev Vet Med33:235–240
10.
IzumiyaYJangHKSugawaraM: 1999, Identification and transcriptional analysis of the homologues of the herpes simplex virus type 1 UL130 to UL140 genes in the genome of nononcogenic Marek's disease virus serotype 2J Gen Virol80:2417–2422
11.
LomontePBublotMVan SantenV: 1995, Analysis of bovine herpesvirus 4 genomic regions located outside the conserved gammaherpesvirus gene blocksJ Gen Virol76:1835–1841
12.
LyakuJRSVilcekSNettletonPFMarsdenHS: 1996, The distinction of serologically related ruminant alphaherpesviruses by polymerase chain reaction (PCR) and restriction endonuclease analysisVet Microbiol48:135–142
13.
MagninoSConzoGFiorettiA: 1996, An outbreak of Pacheco's parrot disease in psittacine birds recently imported to Campania, Italy: isolation of psittacid herpesvirus 2J Vet Med B43:631–637
14.
MurphyFAFauquetCMBishopDHL: 1995, Sixth report of the International Committee on Taxonomy of VirusesArch Virol10 (suppl.): 000–000
15.
RodriguezMSuarezHeinlein ARuizMMetzlerAESchudelAA: 1989, Detection of bovine herpesvirus type 1 via an immunoperoxidase method, using monoclonal antibodiesAm J Vet Res50:619–621
16.
RosCBelakS: 1999, Studies of genetic relationships between bovine, caprine, cervine, and rangiferine alphaherpesviruses and improved molecular methods for virus detection and identificationJ Clin Microbiol37:1247–1253
17.
RosCRiquelmeMEForslundKBelakS: 1999, Improved detection of five closely related ruminant alphaherpesviruses by specific amplification of viral genomic sequencesJ Virol Methods83:55–65
18.
ScottFMM: 1989, Bovine herpesvirus 2 infectionIn: Herpesvirus diseases in cattle, horses, and pigs, ed. WittmannG, pp. 73–95. Kluwer Academic, Boston, MA
19.
ThiryEBublotMDubuissonJPastoretPP: 1989, Bovine herpesvirus 4 (BHV4) infection in cattleIn: Herpesvirus diseases in cattle, horses, and pigs, ed. WittmannG, pp. 96–115. Kluwer Academic, Boston, MA
20.
Van EngelenburgFACMaesRKVan OirschotJTRijsewijkFAM: 1993, Development of a rapid and sensitive polymerase chain reaction assay for detection of bovine herpesvirus type 1 in bovine semenJ Clin Microbiol31:3129–3135
21.
Van OirschotJT: 1996, Infectious bovine rhinotracheitis/infectious pustular vulvovaginitisIn: Manual of standards for diagnostic tests and vaccines, ed. OIE Standards Commission, 3rd ed., pp. 281–290. Office International des Epizooties, Paris, France
22.
VilcekSNettletonPFHerringJAHerringAJ: 1994, Rapid detection of bovine herpesvirus 1 (BHV 1) using polymerase chain reactionVet Microbiol42:53–64
