Histopathologic, immunohistochemical, and ultrastructural evaluations were made of a 6-day-old Holstein calf with severe vacuolation of the neuronal perikarya that was widely distributed throughout the central nervous system. No evidence of storage material within the vacuoles was revealed by histopathologic and ultrastructural examinations. Immunohistochemical and electron microscopic examinations were negative for protease-resistant prion protein and scrapie-associated fibrils, respectively. These results indicate that the clinical signs in this calf were not associated with transmissible spongiform encephalopathy. Neuronal vacuo-lation has not previously been documented in calves.
References
1.
ChaplinMJAldrichAMStackMJ: 1998, Scrapie associated fibril detection from formaldehyde fixed brain tissue in natural cases of ovine scrapie. Res Vet Sci64:41–44.
2.
CharltonKM: 1984, Rabies: spongiform lesions in the brain. Acta Neuropathol63:198–202.
3.
CharltonKMCaseyGAWebsterWABundzaA: 1987, Experimental rabies in skunks and foxes. Pathogenesis of the spongiform lesions. Lab Invest57:634–645.
4.
CutlipRCMillerJMRaceRE: 1996, Experimental transmission of scrapie to cattle. In: Bovine spongiform encephalopathy—the BSE dilemma, ed. GibbsCJ, pp. 92–96. Springer-Verlag, New York, NY.
5.
DavisAJJennyALMillerLD: 1991, Diagnostic characteristics of bovine spongiform encephalopathy. J Vet Diagn Invest3:266–271.
6.
FieldEJ: 1952, Vacuolated cells in the dorsal root ganglion of the rabbit. J Comp Pathol62:249–251.
7.
FoleyGLZacharyJF: 1995, Rabies-induced spongiform change and encephalitis in a heifer. Vet Pathol32:309–311.
8.
HadlowWJ: 1995, Neuropathology and the scrapie-kuru connection. Brain Pathol5:27–31.
9.
HamirANFischerKA: 1999, Neuronal vacuolations in raccoons from Oregon. J Vet Diagn Invest11:303–307.
10.
HamirANHeidelJRPictonRRupprechtCE: 1997, Neuronal vacuolation in raccoons (Procyon lotor). Vet Pathol34:250–252.
11.
KirkwoodJKCunninghamAA: 1999, Scrapie-like encephalopathies (priondiseases) in nondomesticated species. In: Zoo and wild animal medicine: current therapy 4, ed. FowlerMEMillerRE, pp. 662–668. WB Saunders, Philadelphia, PA.
12.
KortzGDMeierWAHigginsRJ: 1997, Neuronal vacuolation and spinocerebellar degeneration in young Rottweiler dogs. Vet Pathol34:296–302.
13.
LancasterMJGillIJHooperPT: 1987, Progressive paresis in Angora goats. Aust Vet J64:123–124.
14.
LaplancheJHunterNShinagawaMWilliamsE: 1999, Scrapie, chronic wasting disease, and transmissible mink encephalopathy. In: Prion biology and disease, ed. PrusinerSB, pp. 393–429. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
15.
McGillISWellsGAH: 1993, Neuropathological findings in cattle with clinically suspect but unconfirmed bovine spongiform encephalopathy. J Comp Pathol108:241–260.
16.
MilartZChomiakM: 1971, Pathological changes in the central nervous system of a bull affected with spastic paresis. Vet Rec88:66–68.
17.
MillerJMJennyALTaylorWD: 1993, Immunohistochemical detection of prion protein in sheep with scrapie. J Vet Diagn Invest5:309–3126.
18.
RyderSJHawkinsSACDawsonMWellsGAH: 2000, The neuropathology of experimental bovine spongiform encephalop-athy in the pig. J Comp Pathol122:131–143.
19.
Van den InghTSGAMMandigersPJJvan NesJJ: 1998, Neuronal vacuolar disorder in young Rottweiler dogs. Vet Rec142:245–257.
20.
WellsGAHScottACJohnsonCT: 1987, A novel progressive spongiform encephalopathy in cattle. Vet Rec121:419–420.
21.
WyattJMPearsonGRSmerdonTN: 1991, Naturally occurring scrapie-like spongiform encephalopathy in five domestic cats. Vet Rec129:233–236.
22.
ZlotnikIRennieJC: 1958, A comparative study of the incidence of vacuolated neurons in the medulla from apparently healthy sheep of various breeds. J Comp Pathol68:411–415.
