American canine hepatozoonosis (ACH), caused by Hepatozoon americanum, is an emerging tick-borne disease of dogs. An indirect enzyme-linked immunosorbent assay (ELISA) that should facilitate diagnosis of infection and study of the epidemiology of ACH has been developed using H. americanum sporozoites as antigen. Efficacy of the new test as a diagnostic tool was compared with that of skeletal muscle biopsy, the current gold standard for confirming H. americanum infection. Results show that the test is sensitive (93%) and specific (96%) and that it is as reliable as histopathologic examination of skeletal muscle for detecting infection. The ELISA would be suitable as a routine laboratory test for diagnosis of ACH.
References
1.
BanethGShkapVSamishM: 1998, Antibody response to Hepatozoon canis in experimentally infected dogs. Vet Parasitol74:299–305.
2.
CraigTMJonesLPNordgrenRM: 1984, Diagnosis of Hepatozoon canis by muscle biopsy. J Am Anim Hosp Assoc20:301–303.
3.
CraigTMSmallwoodJEKnauerKWMcGrathJP: 1978, Hepatozoon canis infection in dogs: clinical, radiographic, and hematological findings. J Am Vet Med Assoc173:967–972.
4.
GossetKAGauntSDAjaDS: 1985, Hepatozoonosis and ehrlichiosis in a dog. J Am Anim Hosp Assoc21:265–267.
5.
GreinerM: 1996, Two-graph receiver operating characteristic (TG-ROC): update version supports optimization of cut-off values that minimize overall misclassification costs. J Immunol Methods191:93–94.
6.
GreinerMSohrDGöbelP: 1995, A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests. J Immunol Methods185:123–132.
7.
JamesSP: 1905, On a parasite found in the white corpuscles of the blood of dogs. Scien Mem Off Med Saint Dept Gov India14:1–12.
8.
JekelJFElmoreJGKatzDL: 1996, Understanding and reducing errors in clinical medicine. In: Epidemiology, biostatistics, and preventive medicine, pp. 85–87. WB Saunders, Philadelphia, PA.
9.
MacintireDK: 1997, Emerging tick-borne diseases. In: International forum on ticks and tick-borne diseases. Compend Cont Ed Pract Vet19:66–70.
10.
MacintireDKVincent-JohnsonNDillonAR: 1997, Hepatozoonosis in dogs: 22 cases (1989–1994). J Am Vet Med Assoc210:916–922.
11.
MathewJSEwingSAPancieraRJKocanKM: 1999, Sporogonic development of Hepatozoon americanum (Apicomplexa) in its definitive host, Amblyomma maculatum (Acarina). J Parasitol85:1023–1031.
12.
MathewJSEwingSAPancieraRJWoodsPJ: 1998, Experimental transmission of Hepatozoon americanum Vincent-Johnson et al., 1997 to dogs by the Gulf Coast tick, Amblyomma maculatum Koch. Vet Parasitol80:1–14.
13.
MathewJSVan Den BusscheRAEwingSA: 2000, Phylogenetic relationships of Hepatozoon (Apicomplexa: Adeleorina) based on molecular, morphologic, and life-cycle characters. J Parasitol86:366–372.
14.
MillerWW: 1908, Hepatozoon perniciosum n. g., n. sp., a haemogregarine pathogenic for white rats; with a brief description of the sexual cycle in the intermediate host, a mite (Laelaps echidninus Berlese). Bull Hyg Lab Wash46:51–123.
15.
PancieraRJEwingSAMathewJS: 1999, Comparison of lesions and parasites in skeletal muscle of dogs experimentally or naturally infected with Hepatozoon americanum. Vet Parasitol82:261–272.
16.
PancieraRJGattoNTCrystalMA: 1997, Canine hepatozoonosis in Oklahoma. J Am Anim Hosp Assoc33:221–225.
17.
SmallwoodJE: 1978, Periosteal new bone formation associated with Hepatozoon gametocytes in two dogs: a preliminary report. J Am Radiol Soc19:142–143.
18.
Vincent-JohnsonNAMacintireDKBanethG: 1997, Canine hepatozoonosis: pathophysiology, diagnosis and treatment. Compend Cont Ed Pract Vet19:51–65.
19.
Vincent-JohnsonNAMacintireDKLindsayDS: 1997, A new Hepatozoon species from dogs: description of the causative agent of canine hepatozoonosis in North America. J Parasitol83:1165–1172.
20.
WenyonCM: 1926, Protozoology: a manual for medical men, veterinarians and zoologists. Bailliere, Tindall and Cassel, London, UK.
