It has been shown that induction of endotoxin tolerance results in a decreased potential to produce pro-inflammatory cytokines such as TNF or IL-1. The present study was carried out to examine the capacity of peripheral blood mononuclear cells (PBMC) from endotoxin tolerant humans to synthesize the anti-inflammatory cytokines IL-10 and TGFβ in response to endotoxin. Four individuals were rendered endotoxin tolerant by daily i.v. injections of endotoxin. PBMC were isolated before tolerance induction and on day 4 after the last endotoxin treatment. The cells were stimulated with endotoxin ex vivo and cytokines in culture supernatants were determined. PBMC isolated on day 4 of all of the volunteers showed decreased TNFα, IL-6 and IL-10 synthesis upon endotoxin treatment in vitro compared to pretolerant cells, while no differences occurred with regard to the amount of TGFβ formed by PBMC before and after tolerance induction. Furthermore, no changes were found in the expression of HLA-DR on PBMC after tolerance induction.
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