Restricted accessResearch articleFirst published online 1994-06
A hamster anti-idiotype monoclonal antibody,mimicking the inner-core region of Gram-negative bacterial lipopolysaccharide (LPS),stimulates LPS inner-core-specific serum antibodies in hamsters
We have earlier described the development of an Ab2β anti-idiotype antibody specific for idiotypic determinants of a BALB/c mouse IgG3 monoclonal antibody (MAbY1-4A6) directed against the conserved inner-core 2-keto-3-deoxyoctulosonate (Kdo) residue of Salmonella minnesota rough mutant Re lipopolysaccharide (Re-LPS) [Field S, Pollack M, Morrison D C. Microb Pathog 1993; 15: 103-120]. Immunization of both inbred and outbred mice with MAb4G2 protected these animals against subsequent lethal challenge with S. minnesota Re-LPS. Here we demonstrate that hamsters immunized with the anti-idiotype MAb4G2 generate serum antibodies which cross-react with a variety of forms of LPS, as evidenced by specific dose-dependent binding to R and S chemotype LPS as assessed by ELISA and specific binding to antibiotic-treated, whole bacterial culture supernatants and to LPS-containing fractions in sucrose gradient velocity-sedimentation-fractionated, antibiotic-treated bacterial culture supernatants. Competitive inhibition assays using MAb4G2-elicited hamster antibodies (Ab3 or Ab1') and the Kdo-specific MAbY1-4A6 (Ab1) were carried out to assess the Kdo epitope-specificity and Ab1'-like properties of the hamster αMAb4G2 antibodies (Ab3). Hamster αMAb4G2 serum antibodies and the Ab1 MAbY1-4A6 express similar epitope specificities for both the MAb4G2 and Re-LPS antigens, confirming the Kdo epitope-specificity of these Ab1' serum antibodies. These data further establish that MAb4G2 is a true internal surrogate image of the conserved inner-core Kdo epitope of LPS.
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