Abstract
A novel two-photon absorption (TPA) material with phenanthrolin as acceptor (BSIPB) has been firstly synthesized and studied as possible fluorescence probe for nucleic acids detection. The single-photon and two-photon fluorescence properties of the dye in the unbound state and in DNA complexes were investigated. Some quantum yields and TPA cross sections at important points were calculated. The results showed that BSIPB can be used as an efficient fluorescent probes for DNA detection and imaging under both single-photon excitation (SPE) and two-photon excitation (TPE).
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