A new enzyme support consisting of magnetic composite microspheres was prepared using artemisia seed gum, chitosan and magnetic fluid as a source, and characterized. The microspheres contained reactive aldehyde groups that react with the free amino groups of the enzymes.
The β-galactosidase was largely immobilized by covalent binding to the surface of the activated magnetic carriers. The factors controlling the relative activity and the enzymic properties of the immobilized β-galactosidase were compared with those of its soluble counterpart, for which ONPG (o-nitrophenol β-D-galactopyranoside) was chosen as a substrate. The pH and thermal stability of the immobilized β-galactosidase were higher than those of the soluble one.
The Michaelis constant, Km, was evaluated both for the immobilized β-galactosidase and for the soluble enzyme. The immobilized type exhibited better environmental adaptability and re-usability than the soluble one.
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