Effects of combined electroacupuncture and exercise training on motor function and microtubule-associated protein 2 expression in the middle and late stages of cerebral infarction in rats

Abstract

Objective:

To evaluate the effects of electroacupuncture (EA) combined with exercise training on motor function and microtubule-associated protein (MAP)-2 in the hippocampal CA3 region of rats in the middle and late stages of cerebral infarction, and explore potential underlying mechanisms of action.

Methods:

A total of 80 Wistar rats were randomly divided into model, EA, training and EA + training groups (n = 20 per group) after establishing the middle cerebral artery occlusion (MCAO) model of cerebral infarction. Rats were treated with EA and/or training in the sixth week post-MCAO. After receiving 2 weeks of treatment, motor function was assessed and MAP-2 expression in the CA3 region was measured using an immunohistochemical method.

Results:

Compared to the model group, significant differences in walking stick, balance beam and screen capture ability were detected in the EA, training and EA + training groups (p < 0.05). The EA + training group showed greater improvements than the EA and training groups (p < 0.05 each). Significant differences in MAP-2 expression were detected in the EA, training and EA + training groups compared to the model group (p < 0.05). MAP-2 expression was higher in the EA + training group than in the EA and training groups (p < 0.05 each).

Conclusion:

MAP-2 expression and motor functional recovery were higher in the combined therapy (EA + training) group compared to the monotherapy (EA or training) groups. EA combined with exercise training appeared to significantly promote the recovery of motor function in the middle and late stages of cerebral infarction in this rat model.