Collagenase and Human Islet Isolation

Abri O. ; Kuhn F. ; Lippert H. The effect of multiple-organ donation on the degree of preservation of isolated pancreas islets. Transplant. Proc. 19: 99–102; 1987.

Arbet-Engels C. ; Darquy S. ; Capron F. ; Reach G. Use of an intracellular solution (University of Wisconsin) for all steps of the isolation of pancreatic islets. Transplant. Proc. 24: 2791; 1992.

Armstrong C.P. ; Taylor T.V. ; Torrance H.B. Pressure, volume and the pancreas. Gut 26: 615–624; 1985.

Au A.M.J. ; Varon S. Neural cell sequestration on immunoaffinity columns. Exp. Cell. Res. 120: 269–276; 1979.

Bedossa P. ; Lemaigre G. ; Bacci J. ; Martin E. Quantitative assessment of the collagen content in normal and pathologic pancreas tissue. Digestion 44: 7–13; 1989.

Benhamou P.Y. ; Watt P.C. ; Mullen Y. Human islet isolation in 104 consecutive cases. Factors affecting isolation success. Transplantation 57: 1804–1810; 1994.

Bodian D. Simplified method of dispersion of monkey kidney cells with trypsin. Virology 4: 575–577; 1956.

Bond M.D. ; Van Wart H.E. Purification and separation of individual collagenases of Clostridium histolyticum using red dye ligand chromotography. Biochemistry 23: 3077–3085; 1984.

Bond M.D. ; Van Wart H.E. Characterization of the individual collagenases from Clostridium histolyticum. Biochemistry 22: 3085–3091; 1984.

Bond M.D. ; Van Wart H.E. Relationship between the individual collagenases of Clostridium histolyticum: Evidence for evolution by gene duplication. Biochemistry 23: 3092–3099; 1984.

Bornstein P. The cross-linking of collagen and elastin and its inhibition in osteolathyrism. Is there a relation to the aging process? Am. J. Med. 49: 429–435; 1970.

Brandhorst D. ; Hering B.J. ; Brandhorst H. ; Federlin K. ; Bretzel R.G. Influence of donor data and organ procurement on human islet isolation. Transplant. Proc. 26: 592–593; 1994.

Brandhorst H. ; Hering B.J. ; Klitscher D. ; Bretzel R.G. ; Federlin K. Impact of cold ischemia and timing of intraductal collagenase distension on human islet yield, purity, viability and survival in low temperature culture. Transplant. Proc. In press; 1994.

Brandhorst H. ; Klitscher D. ; Hering B.J. ; Federlin K. ; Bretzel R.G. Influence of organ procurement on human islet isolation. (Abst.) Horm. Metab. Res. 25: 51–52; 1993.

Burghen G.A. ; Murrell L.R. Factors influencing isolation of islets of Langerhans. Diabetes 38(Suppl. 1): 129–132; 1989.

Burgmann H. ; Reckendorfer H. ; Sperlich M. ; Doleschel W. ; Spieckermann P.G. The calcium chelating capacity of different protecting solutions. Transplantation 54: 1106–1108; 1992.

Carrick L. ; Berk R.S. Purification and partial purification of a collagenolytic enzyme from Pseudomonas aeruginosa. Biochim. Biophys. Acta 391: 422; 1975.

Casanova D. ; Xenos L. ; Lloveras J.J. Comparison of human islet isolation from the stored and non-stored pancreas with two different protocols using UW solution. Transplant. Proc. 26: 588; 1994.

Cavanagh T.J. ; Fetterhoff T.J. ; Lonergan S.C. ; Dwulet F.E. ; Gill J.F. ; McCarthy R.C. Collagenase selection. In: Lanza R.P. ; Chick W.L. , eds. Pancreatic islet transplantation: Procurement of pancreatic islets. Austin: R.G. Landes; 1994: 39–51.

Cawston T.E. ; Barrett A.J. A rapid and reproducible assay for collagenase using [1-14C]-acetylated collagen. Anal. Biochem. 99: 340–345; 1979.

Cawston T.E. ; Murphy G. Mammalian collagenases. In: Lorand L. , ed. Methods in enzymology: Proteolytic enzymes. Vol. 80, Part C. New York: Academic Press; 1981; 711–722.

Chadwick D.R. ; Robertson G.S.M. ; Contractor H.H. Storage of pancreatic digest prior to islet purification; the influence of colloids and the sodium-potassium ratio in UW based preservation solutions. Transplantation 58: 99–104; 1994.

Chadwick D.R. ; Robertson G.S.M. ; Rose S. Storage of porcine pancreatic digest prior to islet purification: The benefits of UW solution and the role of its individual components. Transplantation 56: 288–293; 1993.

Chavira R. ; Burnett T.J. ; Hageman J.H. Assaying proteinases with Azacoll. Anal. Biochem. 136: 446–450; 1984.

Compton F. ; Miller B.H.R. ; Bewick M. Isolation, culture and cryopreservation of islets and the endocrine pancreas. The fractionation of crude collagenase for pancreatic islet isolation. Horm. Metabl. Res. 12: 55–58, 1982.

Contractor H.H. ; Bhalla R. ; James R.F.L. ; Robertson G.S.M. ; Chadwick D.R. ; London N.J.M. The effect of UW solution and its components on the collagenase digestion of the porcine and human pancreas. Transplant. Proc. 26: 587; 1994.

Contractor H.H. ; Johnson P.R.V. ; Chadwick D.R. ; Robertson G.S.M. ; London N.J.M. The effect of UW solution and its components on collagenase digestion of human and porcine pancreas. Cell Transplantation In press; 1995.

Corlett M.P. ; Scharp D.W. The effect of pancreatic warm ischemia on islet isolation in rats and dogs. J. Surg. Res. 45: 531–536; 1988.

Desnuelle P. Trypsin. In: Boyer P.D. ; Lardy H. ; Myrback K. , eds. The enzymes. New York: Academic Press; 1960; 119.

Dono K. ; Gotoh M. ; Fukuzaki T. The role of calcium in collagenase digestion and preservation of islets. Transplant. Proc. 24: 1000–1001; 1992.

Dono K. ; Gotoh M. ; Monden M. ; Kanai T. ; Fukuzaki T. ; Mori T. Low temperature collagenase digestion for islet isolation from 48-hour cold-preserved rat pancreas. Transplantation 57: 22–26; 1994.

Downing R. ; Scharp D.W. ; Ballinger W.F. An improved technique for the isolation and identification of mammalian islets of Langerhans. Transplantation 29: 79–83; 1980.

Downing R. ; Scharp D.W. ; Merrell R.C. ; Grieder M. ; Ballinger W.F. Venous vs. ductal distension of the canine pancras for isolation of islets. Surg. Forum 30: 422–424; 1979.

Farooq M. ; Norton W.T. A modified procedure for isolation of astrocyte- and neuron-enriched fractions from rat brain. J. Neurochem. 31: 887–894; 1978.

Fedoroff S. ; Hall C. Effect of horse serum on neural cell differentiation in tissue culture. In Vitro 15: 641–648; 1979.

Fetterhoff T.J. ; Cavanagh T.J. ; Wile K.J. Human pancreatic dissociation using a purified enzyme blend. Transplant. Proc. 27(6): 3282–3283, 1995.

Garrod D.R. ; Nicol A. Cell behaviour and the molecular mechanisms of cell-cell adhesion. Biol. Rev. 56: 199; 1981.

Garthwaite L. ; Balazs R. Separation of cell types from the cerebellum and their properties. Adv. Cell. Neurobiol. 2: 461–489; 1981.

Gill J.H. ; Love L.A. ; Baurley J. Safety testing of Liberase, a purified enzyme blend for human islet isolation. Transplant. Proc. In press; 1995.

Goodenough D.A. ; Gilula N.B. The splitting of hepatocyte gap junctions and zonae occludentes with hypertonic disaccharides. J. Cell Biol. 61: 575–590; 1974.

Gores P.F. ; Sutherland D.E.R. Commentary. Cell Transplantation 2: 291–293; 1993.

Goss J. ; Harper E. ; Harris E.D. Animal collagenases: Specificity of action and structures of the substrate cleavage sites. Biochem. Biophys. Res. Commun. 61: 605–612; 1974.

Gotoh M. ; Maki T. ; Kiyoizumi T. ; Satomi S. ; Monaco A.P. An improved method for isolation of mouse pancreatic islets. Transplantation 40: 437–438; 1985.

Gotoh M. ; Maki T. ; Satomi S. Reproducible high yield of rat islets by stationary in vitro digestion following pancreatic ductal or portal venous collagenase injection. Transplantation 43: 725–730; 1987.

Grand C.G. ; Maddi F. The effects of various concentrations of calcium chloride on the intercellular cement of endothelial cells grown in vitro. Anat. Rec. 112: 334; 1952.

Grant N.H. ; Album, Studies on the collagenases of Clostidium histolyticum. H.E. Arch. Biochim. Biophys. 82: 245–255; 1959.

Gray D.W.R. Approaches to pancreatic tissue dispersion. In: Lanza R.P. ; Chick W.L. , eds. Pancreatic islet transplantation: Procurement of pancreatic islets. Austin: R.G. Landes Company; 1994; 11–23.

Gray D.W.R. ; Leow C.K. Non-automated methods for islet isolation. In: Ricordi C. , ed. Pancreatic islet cell transplantation. Austin: R.G. Landes Company, 1992; 89–98.

Gray D.W.R. ; McShane P. ; Grant A. ; Morris P.J. A method for isolation of islets of Langerhans from the human pancreas. Diabetes 33: 1055–1061; 1984.

Harper E. ; Berger A. ; Katchalski E. The hydrolysis of poly(L-prolyl-glycyl-L-prolyl) by bacterial collagenase. Biopolymers 11: 1607–1612; 1972.

Harper E. ; Kang A.H. Studies on the specificity of bac- terial collagenase. Biochem. Biophys. Res. Commun. 41: 482–487; 1970.

Harris C.C. ; Leone C.A. Some effects of EDTA and tetraphenylboron on the ultrastructure of mitochondria in mouse liver cells. J. Cell Biol. 28: 405–408; 1966.

Hefley T.H. Utilization of FLPC-purified bacterial collagenase for the isolation of cells from bone. J. Bone Mineral Res. 2: 505–516; 1987.

Heiser A. ; Ulrichs K. ; Eckstein V. ; Muller Ruchholtz W. Xenogeneic cellular response of human lymphocytes to porcine lymphocytes and isolated porcine pancreatic islets. Transplant. Proc. 24: 2881–2882; 1992.

Heiser A. ; Ulrichs K. ; Muller-Ruchholtz W. Isolation of porcine pancreatic islets: Low trypsin activity during the isolation procedure guarantees reproducible islet yields. J. Clin. Lab. Anal. 8: 407–411; 1994.

Heiser A. ; Ulrichs K. ; Muller-Rucholtz W. Enzyme kinetics of commercial collagenase and their influence on porcine pancreatic islet isolation. Cell Transplantation 3: 242; 1994.

Heiser A. ; Ulrichs K. ; Muller-Rucholtz W. Enzyme activities in commercial collagenase preparations and their kinetics during islet isolation procedure. Horm. Metabl. Res. 26: 67; 1994.

Hellerstrom C. A method for the microdissection of intact pancreatic islets of mammals. Acta Endocrinologica 45: 122–132; 1964.

Henn F.A. Separation of neuronal and glial cells and subcellular constituents. Adv. Cell. Neurobiol. 1: 373–403; 1980.

Henriksson C. ; Bergmark J. ; Claes G. Use of trypsin for isolation of islets of Langerhan in the rat. Eur. Surg. Res. 9: 427–431; 1977.

Henry H. J. Path. Bacteriol. 26: 497; 1923.

Hesse F. ; Burtscher H. ; Popp F. ; Ambrosius D. Recombinant enzymes for islet isolation: Purification of a collagenase from Clostridium histolyticum and cloning/expression of the gene. Transplant. Proc. 27(6): 3287–3289, 1995.

Hilfer S.R. Collagenase treatment of chick heart and thyroid. In: Kruse P.F. ; Patterson M.K. , eds. Tissue culture: Methods and applications. New York: Academic Press; 1973; 16–20.

Hinshaw D.B. ; Jolley W.B. ; Knierim K.H. New nonenzymatic method for the isolation of functional pancreatic islets. Surg. Forum 32: 381–383; 1981.

Hinz R.W. ; Syverton J.T. Mammalian cell cultures for study of influenza virus. I. Preparations of monolayer cultures with collagenase. Proc. Soc. Exp. Biol. Med. 101: 19–22; 1959.

Hodges G.M. ; Livingstone D.C. ; Franks L.M. The localization of trypsin in cultured mammalian cells. J. Cell Sci. 12: 887–902; 1973.

Horaguchi A. ; Merrell R.C. Preparation of viable islets cells from dogs by a new method. Diabetes 30: 455–458; 1981.

Hosick H.L. ; Strolman R. Changes in ribosomepolyribosome balance in chick muscle cells during tissue dissociation, development in culture, and exposure to simplified culture medium. J. Cell. Physiol. 77: 145–156; 1973.

Howart H.T. ; Sales H. The exocrine pancreas. 2nd ed. London: W.B. Saunders Co. Ltd.; 1979.

Jingu K. ; Asano T. ; Kenmochi K. Combined method of mechanical chopper and automated digestion system for islet isolation. Transplant. Proc. 26: 634–636; 1994.

Johnson P.R.V. ; Button C.L. ; Roberts D.L. University of Wisconsin solution inhibits the class II collagenase within crude Clostridium histolyticum collagenase. Transplant. Proc. 27(6): 3286. 1995.

Johnson P.R.V. ; van Suylichem P.T.R. ; Roberts D.L. A simple, “in-vitro” method for evaluating the efficacy of different batches of crude Clostridium histolyticum collagenase for islet isolation. Transplant. Proc. 27(6): 3284–3285, 1995.

Johnson P.R.V. ; van Suylichem P.T.R. ; Roberts D.L. Design of a simple, “in vitro” method for evaluating the efficiency of crude Clostridium histolyticum collagenase and its components for porcine islet isolation. Xenotransplantation 27(6): 3286, 1995.

Juurlink B.H.J. ; Fedoroff S. ; Hall C. Nathaniel, E.J.H. Astrocyte cell lineage. I. Astrocyte progenitor cells in mouse neopallium. J. Comp. Neurol. 200: 375–391; 1981.

Kane R.E. Hyalin release during normal sea urchin development and its replacement after removal at fertilization. Exp. Cell Res. 81: 301–311; 1973.

Kasten F.H. Rat myocardial cells in vitro: Mitosis and differentiated properties. In Vitro 8: 128–149; 1972.

Kasten F.H. Preparation of primary cultures. Mammalian myocardial cells. In: Kruse P.F. ; Patterson M.K. , eds. Tissue culture: Methods and applications. New York: Academic Press; 1973: 72–81.

Keil B. ; Gilles A.M. ; Lecroisey A. ; Hurion N. ; Tong N.T. Specificity of collagenase from Achromobacter iophagus. FEBS Lett. 56: 292–296; 1975

Keil-Dlouha V. Chemical characterization and the study of the autodigestion of pure collagenase from Achromobacter iophagus. Biochem. Biophys. Acta 429: 239–251; 1976.

Kennedy R.H. ; Bockman D.E. ; Uscanga L. ; Choux R. ; Grimaud J.A. ; Sarles H. Pancreatic extracellular matrix alterations in chronic pancreatitis. Pancreas 21: 61–72; 1987.

Ketchum R.J. ; Nicolae M. ; Jahr H. Analysis of donor age and cold ischaemia time as factors in cadaveric human islet isolation. Transplant. Proc. 26: 596–597; 1994.

Kleinman H.K. ; Klebe R.J. ; Martin G.R. Role of collagenous matrices in the adhesion and growth of cells. J. Cell Biol. 88: 473–185; 1981.

Klitscher D. ; Brandhorst H. ; Hering B. ; Federlin K. ; Bretzel R.G. Can donor data predict human islet isolation outcome? Horm. Metab. Res. 25: 60; 1993.

Klitscher D. ; Hering B.J. ; Brandhorst H. ; Schede C.P. ; Bretzel R.G. ; Federlin K. Influence of donor data and organ procurement on human islet isolation. Presented at the 4th International Congress on Pancreas and Islet Transplantation. Amsterdam; 1993.

Kneteman N.M. ; DeGroot T.J. ; Warnock G.L. ; Rajotte R.V. The evaluation of solutions for pancreas preservation prior to islet isolation. Horm. Metab. Res. Suppl. 25: 4–9; 1990.

Kneteman N.M. ; Lakey J.R.T. ; Warnock G.L. ; Rajotte R.V. Human islet isolation after prolonged cold storage. Diab. Nutr. Metab. 5(Suppl. 1): 33–37; 1992.

Kono T. Purification and partial characterisation of collagenolytic enzymes from Clostridium histolyticum. Biochemistry 7: 1106–1114; 1968.

Kono T. Roles of collagenases and other proteolytic enzymes in the dispersal of animal tissues. Biochim. Biophys. Acta 178: 397–400; 1969.

Krause U. ; Puchinger H. ; Wacker A. Inhibition of glucose-induced insulin secretion in trypsin-treated islets of Langerhans. Horm. Metab. Res. 5: 325–329; 1973.

Lacy P.E. ; Kostianovsky M. Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16: 35–39; 1967.

Lakey J.R.T. ; Kneteman N.M. ; Rajotte R.V. ; Kizilisik T. ; Ao Z. ; Warnock G.L. A prospective evaluation of two techniques for human islet isolation. Transplant. Proc. 26: 589; 1994.

Lecroisey A. ; Keil-Dlouha V. ; Woods D.R. ; Perrin D. ; Keil B. Purification, stability and inhibition of the collagenase from Achromobacter iophagus. FEBS Lett. 59: 167–172; 1975.

Lernmark A. The preparation of, and studies on, free cell suspensions from mouse pancreatic islets. Diabetologia 10: 431–438; 1974

Lin H-C. ; Lei S.-P. Molecular cloning of the genes responsible for collagenase production from Clostridium histolyticum. World Intellectual Property Organization Patent Application WO 94/00580; 1994.

Lin Y. ; Means G.E. ; Feeney R.E. The action of proteolytic enzymes on N,N-dimethyl proteins. Basis for a microassay for proteolytic enzymes. J. Biol. Chem. 244: 789–793, 1969.

Linetsky E. ; Selvggi G. ; Bottino R. Comparison of collagenase type P and Liberase during human islet isolation using the automated method. Transplant. Proc. 27(6): 3264, 1995.

London N.J.M. ; Johnson P.R.V. ; Robertson G.S.M. ; Chadwick D.R. ; White S.A. Approaches to islet purification. In: Lanza R.P. ; Chick W.L. , eds. Pancreatic islet transplantation: Procurement of pancreatic islets. Austin: R.G. Landes Company; 1994: 25–38.

London N.J.M. ; Robertson G.S.M. ; Chadwick D.R. ; Johnson P.R.V. ; James R.F.L. ; Bell P.R.F. Human pancreatic islet isolation and transplantation. Clin. Transplant. 8: 421–459; 1994.

Longmuir I.S. ; Rees W. Preparation of cell suspensions from rat liver. Nature 177: 997; 1956.

Lwebuga-Mukasa J.S. ; Harper E. ; Taylor P. Collagenase enzymes from Clostridium: Characterisation of individual enzymes. Biochemistry 15: 4736–4741; 1976.

MacFarlane R.G. ; MacLennan J.D. Toxaemia of gas gangrene. Lancet 2: 328–331; 1945.

Mallya S.K. ; Mookhtiar K.A. ; Van Wart H.E. Accurate quantative assays for the hydrolysis of soluble type 1, 2, and 3 3H-acetylated collagens by bacterial and tissue collagens. Anal. Biochem. 158: 334–345; 1986.

Mandl I. ; Keller S. ; Manahan J. Multiplicity of Clostridium histolyticum collagenases. Bichemistry 3: 1737–1741; 1964.

Mandl I. ; Zipper H. ; Ferguson L.T. Clostridium histolyticum collagenase: Its purification and properties. Arch. Biochem. 74: 465–475; 1958.

Maschmann E. Biochem. Z. Zur kenntnis der proteasen des B. sporogenes Übereine neue proteinase und übes akti viebare peptidasen. Biochem. Ztsches. 300: 89–112, 1939.

Mazia D. The particulate organization of the chromosome. Proc. Natl. Acad. Sci. USA 40: 521–527; 1954.

McClay D. ; Marchese R.B. Separation of ectoderm and endoderm from sea urchin pluteus larvae and demonstration of germ layer-specific antigens. Dev. Biol. 71: 289–296; 1979.

McShane P. ; Sutton R. ; Gray D.W.R. ; Morris P.J. Protease activity in pancreatic islet isolation by enzymatic digestion. Diabetes 38(Suppl. 1): 126–128; 1989.

Mellert J. ; Hering B.J. ; Hopt U.T. Exchange of pancreata and islets between centers for experimental islet transplantation in the pig. Transplant. Proc. 23: 2435–2436; 1991.

Mendola G. ; Casamitjana R. ; Gomis R. Effect of nicotinamide therapy upon B cell function in newly diagnosed Type 1 (insulin-dependent) diabetic patients. Diabetologia 32: 160–162; 1989.

Mitchell W.M. ; Harrington W.F. Purification and properties of clostridiopeptidase B (clostripain). J. Biol. Chem. 243: 4683; 1968.

Montes de Oca H. Trypsin. B. High yield method for kidney tissue. In: Kruse P.F. ; Patterson M.K. , eds. Tissue culture: Methods and applications. New York: Academic Press; 1995; 8–12.

Mookhtiar K.A. ; Steinbrink D.R. ; Van Wart H.E. Mode of hydrolysis of collagen-like peptides by class I and class II Clostridium histolyticum collagenases: Evidence for both endopeptidase and tripeptidylcarboxypeptidase activities. Biochemistry 24: 6527–6533; 1985.

Morrow C.E. ; Sutherland D.E.R. ; Steffes M.W. ; Najarian J.S. ; Bach F.H. H-2 antigen class: Effect on mouse islet allograft rejection. Science 219: 1337–1339; 1983.

Moskalewski S. Isolation and culture of the islets of Langerhans of the guinea pig. Gen. Comp. Endocrinol. 5: 342–353; 1965.

Munn S.R. ; Kaufman D.B. ; Field M.J. ; Viste A.B. ; Sutherland D.E. Cold-storage preservation of the canine and rat pancreas prior to islet isolation. Transplantation 47: 28–31; 1989.

Nakas M. ; Higashino S. ; Loewenstein W.R. Uncoupling of an epithelial cell membrane junction by calcium removal. Science 151: 89–91; 1966.

Noel J. ; Rabinovitch A. ; Olsen L. ; Kyriakides G. ; Miller J. ; Mintz D.H. A method for large-scale, high-yield isolation of canine pancreatic islets of Langerhans. Metabolism 31: 184–187; 1982.

Ohzato H. ; Gotoh M. ; Monden M. ; Dono K. ; Kanai T. ; Mori T. Improvement in islet yield from a cold-preserved pancreas by pancreatic ductal collagenase distention at the time of harvesting. Transplantation 51: 566–570; 1991.

Ohzato H. ; Gotoh M. ; Monden M. Intraductal injection of collagenase solution at the time of harvesting: A possible solution for preservation and collagenase digestion. Transplant. Proc. 22: 782–783; 1990.

Oshima G. ; Shimabukuro H. ; Nagasawa K. Mode of action of bacterial collagenase on a synthetic substrate, (Pro-Pro-Gly)5. Biochim. Biophys. Acta 567: 392–400; 1979.

Peterkofsky B. In: Cunningham L.W. ; Frederiksen D.W. ; eds. Methods in enzymology. New York: Academic Press, 82, Part A: 453–471; 1982.

Pine L. ; Bradley G. ; Miller D. Collagenolytic activity and comparison of good and bad lots of commercial trypsin in the preparation of primary monkey kidney cells. Cytobios 4: 347–367; 1969.

Pine L. ; Taylor G.C. ; Miller D.M. ; Bradley G. ; Wetmore H.R. Comparison of good and bad lots of trypsin used in the production of primary monkey kidney cells. A definition of the problem and comparison of certain enzymatic characteristics. Cytobios 2: 197–207; 1969.

Pirola R.C. ; Davis A.E. Effect of pressure on the integrity of the duct-acinar system of the pancreas. Gut 11: 69–73; 1970.

Poduslo S.E. ; Norton W.T. Isolation of specific brain cells. Meth. Enzymol. 35: 561–579; 1975.

Queral A.E. ; DeAngelo A.B. ; Garrett C.T. Effect of different collagenases on the isolation of viable hepatocytes from rat liver. Anal. Biochem. 138: 235–237; 1984.

Rajotte R.V. ; Warnock G.L. ; Evans M.G. Ellis, D.; Davidson, I. Isolation of viable islets of Langerhans from collagenase-perfused canine and human pancreas. Transplant. Proc. 19: 918–922; 1987.

Rick W. Enzyme activities: Trypsin. In: Methods in enzymology. New York: Academic Press; 1993.

Ricordi C. The automated method for islet isolation. In: Ricordi C. , ed. Pancreatic islet cell transplantation. 1892-1992: One century of transplantation for diabetes. Austin: R.G. Landes; 1992: 99–112.

Ricordi C. ; Alejandro R. ; Rilo H.L. Long term in-vivo function of human mantled islets obtained by incomplete Pancreatic dissociation and purification. Transplant. Proc. 27(6): 3379, 1995.

Ricordi C. ; Lacy P.E. ; Finke E.H. ; Olack B.J. ; Scharp D.W. Automated method for isolation of human pancreatic islets. Diabetes 37: 413–420; 1988.

Ricordi C. ; Lacy P.E. ; Scharp D.W. Automated islet isolation from human pancreas. Diabetes 38(Suppl. 1): 140–142; 1989.

Ricordi C. ; Socci C. ; Davalli A.M. Effect of pancreas retrieval procedure on islet isolation in the swine. Transplant. Proc. 22: 442–443; 1990.

Robertson G.S.M. ; Chadwick D.R. ; Thirdborough S. Human islet isolation: A prospective randomised comparison of pancreatic vascular perfusion with hyperosmolar citrate or University of Wisconsin solution. Transplantation 56: 550–552; 1993.

Rose S.P.R. Preparation of enriched fractions from cerebral cortex containing metabolically active neuronal cells. Nature 206: 621–622; 1965.

Rous P. ; Jones F.S. A method for obtaining suspensions of living cells from the fixed tissues and for plating out of the individual cells. J. Exp. Med. 23: 549–555; 1916.

Sayers G. ; Beall R.J. ; Seeling S. ; Cummins K. Isolation of adrenal cortex cells—Hormone responses. Meth. Enzymol. 32: 673–693; 1974.

Sayers G. ; Swallow R.L. ; Giordano N.D. An improved accurate and specific method for the assay of ACTH. Endocrinology 88: 1063–1068; 1971.

Scharp D.W. The elusive human islet: Variables involved in its effective recovery. In: van Schilfgaarde R. ; Hardy M.A. , eds. Transplantation of the endocrine pancreas in diabetes mellitus. Amsterdam: Elsevier; 1988: 97–107.

Scharp D.W. Commentary. Cell Transplantation 2: 299; 1993.

Schellman G. ; Fisher E. A collagenase from Pseudomonas aeruginosa. Biochim. Biophys. Acta 122: 557; 1966.

Seglen P.O. Preparation of isolated rat liver cells. Meth. Cell Biol. 13: 29; 1976.

Seifter S. ; Harper E. Collagenases. In: Colowick S.P. , ed. Methods enzymol. Vol. 19. New York: Academic Press; 1970: 613–635.

Seifter S. ; Harper E. The collagenases. In: Boyer P.D. , ed. The enzymes. Vol. III: Hydrolysis: Peptide bonds. New York: Academic Press; 1971: 649–693.

Seifter S. ; Takahashi S. ; Harper E. Further evidence that cysteine reacts with the metal component of collagenase. Biochim. Biophys. Acta 214: 559–561; 1970.

Sellinger O.Z. ; Azcurra J.M. Bulk separation of neuronal cell bodies and glial cells in the absence of added digestive enzymes. In: Marks N. ; Rodright R. , eds. Research methods in neurochemistry. New York: Plenum; 1974: 3–38.

Simms H.S. ; Stillman N.P. Substances affecting adult tissue in vitro. 1. The stimulating action of trypsin on fresh tissue. J. Gen. Physiol. 201: 603–619; 1937.

Snow C. ; Allen A. The release of radioactive nucleic acids and mucoproteins by trypsin and ethylenediaminetetraacetate treatment of baby hamster cells in tissue culture. Biochem. J. 119: 707–714; 1970.

Soberano M.E. ; Schoellmann G. Specificity of bacterial collagenase: Studies with peptides newly synthesized using the solid-phase method. Biochim. Biophys. Acta 271: 133–144; 1972.

Socci C. ; Vignali A. ; Davalli A.M. Timing of collagenase injection influences the final islet yield in donors under 30 years of age. Transplant. Proc. 24: 2835–2836; 1992.

Soru E. ; Zaharia O. Clostridium histolyticum collagenase II. Partial characterisation. Enzymologia 43: 45–55; 1972.

Speicher D.W. ; McCarl R.L. Pancreatic enzyme requirements for the dissociation of rat hearts for culture. In Vitro 10: 30–41; 1974.

Speicher D.W. ; McCarl R.L. Isolation and characterisation of the proteolytic enzyme component from commercially available crude trypsins. Anal. Biochem. 84: 205–217; 1978.

Speicher D.W. ; McCarl R.L. Evaluation of a proteolytic enzyme mixture isolated from trypsins in tissue disaggregation. In Vitro 14: 849–853; 1978.

Steinbrink D.R. ; Bond M.D. ; Van Wart H.E. Substrate specificity of beta-collagenase from Clostridium histolyticum. J. Biol. Chem. 260: 2771–2776; 1985.

Steven F.S. ; Torre-Blanco A. ; Hunter J.A.A. A neutral protease in rheumatoid synovial fluid capable of attacking the telopeptide regions of polymeric collagen fibrils. Biochim. Biophys. Acta 405: 188–200; 1975.

Todd K. ; Kleinman R. ; Brunicardi F.C. Influence of pre-operative donor factors on the performance of the isolated perfused human pancreas. Transplant. Proc. 26: 552; 1994.

Toledo-Pereyra L.H. ; Zammit M. ; Malcom S. ; Cromwell P. Inconsistency of collagenase activity for the isolation of islet cells for transplantation. Transplantation 27: 222; 1979.

Toomey P. ; Contractor H. ; James R.F.L. ; London N.J.M. Cold storage of the human pancreas prior to islet isolation. Presented at the third International Congress on Pancreas and Islet Transplantation. Lyon; 1991.

Traverso L.W. ; Abou-Zamzam A.M. Activation of pancreatic proteolytic enzymes by commercial collagenases. Transplantation 25: 226–227; 1978.

Twining S.S. Fluorescein isothiocyanate-labelled casein assay for proteolytic enzymes. Anal. Biochem. 143: 30–34; 1984.

Tytell A.A. ; Hewson K. Production, purification, and some properties of Clostridium histolyticum collagenase. Proc. Soc. Exp. Biolmed. 74: 555–558; 1950.

Uscanga L. ; Kennedy R.H. ; Stocker S. ; Grimaud J.A. ; Sarles H. Immunolocalization of collagen types, laminin and fibronectin in the normal pancreas. Digestion 30: 158–164; 1984.

van Deijnen J.H. ; Hulstaert C.E. ; Wolters G.H. ; van Schilfgaarde R. Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig, and man. Cell Tissue Res. 267: 139–146; 1992.

van Suylichem P.T. ; Pasma A. ; Wolters G.H. ; van Schilfgaarde R. Microscopic aspects of the structure and collagen content of the pancreas from the perspective of islet isolation. Transplant. Proc. 19: 3958–3959; 1987.

van Suylichem P.T.R. ; Wolters G.H.J. ; van Schilfgaarde R. Peri-insular presence of collagenase during islet isolation procedures. J. Surg. Res. 53: 502–509; 1992.

Van Wart H.E. ; Bond M.D. An accurate, quantitative assay for collagenase activity based on the synertistic hydrolysis of collagen. Anal. Biochem. 120: 151–158; 1982.

Van Wart H.E. ; Steinbrink D.R. A continuous spectrophotometric assay for Clostridium histolyticum collagenase. Anal. Biochem. 113: 356–365; 1981.

Van Wart H.E. ; Steinbrink D.R. Complementary substrate specificities of class I and class II collagenase from Clostridium histolyticum. Biochemistry 24: 6520–6526; 1985.

Vencill C.F. ; Rasnick D. ; Crumley K.V. ; Nishino N. ; Powers J.C. Clostridium histolyticum collagenase: Development of new thio ester, fluorogenic, and depsipeptide substrates and new inhibitors. Biochemistry 24: 3149–3157; 1985.

Venter B.R. ; Venter J.C. ; Kaplan N.O. Affinity isolation of cultured tumor cells by means of drugs and hormones covalently bound to glass and Sepharose beads. Proc. Natl. Acad. Sci. USA 73: 2013–2017; 1976.

Vos-Scheperkeuter G.H. ; Vonk M.W.A. ; Wolters G.H.J. ; van Schilfgaarde R. Collagen degradation by three Clostridium histolyticum collagenase fractions with different substrate specificities. Transplant. Proc. 26(2): 641–642, 1994.

Warnock G.L. ; Ellis D. ; Rajotte R.V. ; Dawidson I. ; Baekkeskov S. ; Egebjerg J. Studies of the isolation and viability of human islets of Langerhans. Transplantation 45: 957–963; 1988.

Warnock G.L. ; Ellis D.K. ; Cattral M. ; Untch D. ; Kneteman N.M. ; Rajotte R.V. Viable purified islets of Langerhans from collagenase-perfused human pancreas. Diabetes 38: 136–139; 1989.

Watt P.C. ; Mullen Y. ; Benhamou P.Y. Donor factors affecting successful isolation of islets from the human pancreas. Transplant. Proc. 26: 594–595; 1994.

Waymouth C. Methods for obtaining cells in suspension from animal tissues. In: Pretlow T.G. ; Pretlow T.P. , eds. Cell separation—Methods and selected applications. Vol. 1. New York: Academic Press; 1982: 1–29.

Welton R.L. ; Woods D.R. Collagenase production by A chromobacter iophagus. Biochem. Biophys. Acta 384: 228–234; 1975.

White S.A. ; Hughes D.P. ; Contractor H.H. the intraductal administration of collagenase in UW at the time of harvesting significantly improves porcine islet isolation. Transplant. Proc. 27(6): 3366, 1995.

Wile K.J. ; Fetterhoff T.J. ; Cavanagh T.J. ; Wright M.J. Image analysis of human pancreatic islets: A time course approach to the evaluation of pancreatic dissociations. Transplant. Proc. 27(6): 3246–3247, 1995.

Wolters G.H. ; van Suylichem P.T. ; van Deijnen J.H. ; van Schilfgaarde R. Factors influencing the isolation process of islets of Langerhans. Horm. Metab. Res. Suppl. 25: 20–26; 1990.

Wolters G.H.J. ; van Suylichem P.T.R. ; van Deijnen J.H.M. ; van Schilfgaarde R. Increased islet yield by im-proved pancreatic tissue dissociation: The effects of bovine serum albumin and calcium. Transplant. Proc. 21: 2626–2627; 1989.

Wolters G.H.J. ; Vos-Scheperkeuter G.H. ; van Deijnen J.H.M. ; van Schilfgaarde R. An analysis of the role of collagenase and protease in the enzymatic dissociation of the rat pancreas for islet isolation. Diabetologia 35: 735–742; 1992.

Wolters G.H.J. ; Vos-Scheperkeuter G.H. ; van Suylichem P.T.R. ; Lin H.C. ; van Schilfgaarde R. Influence of the collagenase/protease ratio and concentrations on pancreatic tissue dissociation. Transplant. Proc. 1994; in press.

Wunsch E. ; Heidrich H.C. An assay for type 1 collagenase. Z. Physiol. Chem. 333: 149–159; 1963.

Yao Q.X. ; Yao Z. ; Heintz R. Effect of donor age and cold ischemia time (CIT) on yield, purity and function of human islets isolated from fifty-four (54) consecutive pancreas donors. Presented at the 4th International Congress on Pancreas and Islet Transplantation. Amsterdam; 1993.

Yoshihara K. ; Matsushita D. ; Minami J. ; Okabe A. Cloning and nucleotide-sequence analysis of the coIH gene from Clostridium histolyticum encoding a collagenase and a gelatinase. J. Bacteriol. 176: 6489–6496; 1994.

Zeng Y. ; Torres M.A. ; Thistlewaite R.J. Correlation between donor characteristics and human pancreatic islet yield and purity. Presented at the 4th International Congress on Pancreas and Islet Transplantation. Amsterdam; 1993.

Zimmerman B. ; Ashe E.C. ; Yurewicz E.C. ; Patel G. Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substrates. Anal. Biochem. 78: 47–51; 1977.