The frequency and the specificities of antinuclear antibodies (ANAb) were studied in dogs with systemic lupus erythematosus (SLE) and compared to those found in normal dogs and in dogs with various infectious diseases. Whole ANAb were detected by immunofluorescence. Anti-double-stranded DNA Ab were found in only 2% of SLE dogs, whereas anti-single-stranded DNA Ab were present in 21.4% of SLE dogs and in 26.8% of dogs with infectious disease. Antihistone Ab were frequently observed in SLE dogs (71%) and are essentially directed against trypsin-resistant epitopes of H3, H4 and H2A. The Western blots of nuclear extracts of HeLa cells were recognized mainly by type 1 Ab (30%, reacting with bands of 43, 36, 35, 34, 30 and 27 kDa) and by anti-Sm Ab (12%) associated with anti-RNP Ab. Anti-SSA and anti-SSB Ab were rare.
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Lapras M., Monier JCTechnique d'IF appliquée au diagnostic des maladies autoimmunes chez le chien: résultats cliniques. Rev Med Vet1971; 120: 973-89.
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Grinden CB, Johnson KHSystemic lupus erythematosus: literature review and report of 42 new canine cases. J Am Anim Hosp Assoc1983; 19: 489-503.
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Hubert B., Teichner M., Fournel C., Monier JCSpontaneous familial SLE in a canine breeding colony. J Comp Pathol1988; 98: 81-9.
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Costa 0, Fournel E, Lotchouang JC, Monier JC, Fontaine M. Specificities of antinuclear antibodies detected in dogs with systemic lupus erythematosus. Vet Immunol Immunopathol1984; 7: 369-82.
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Brinet A., Fournel C., Faure JR, Venet C., Monier JCAntihistone antibodies (ELISA and immunoblot) in canine lupus erythematosus . Clin Exp Immunol1988; 74: 105-9.
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Ait-Kaci A., Monier JC, Mamelle N.Enzyme linked immunosorbent assay for antihistone antibodies and their presence in systemic lupus erythematosus sera. J Immunol Meth1981; 44: 311-22.
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Hardin JA, Thomas JOAntibodies to histones in systemic lupus erythematosus: localization of prominent autoantigens on histones H1 and H2B. Proc Natl Acad Sci USA1983; 80: 7410-4.
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Gioud M., Ait-Kaci A, Monier JC. Histone antibodies in systemic lupus erythematosus . Arthritis Rheum1978; 25: 407-13.
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Tan EM, Cohen AS, Fries JF et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum1982; 25: 1271-7.
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Johns EW, ed. The HMG Chromosomal Proteins . New York: Academic Press, 1982.
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Briolay J., Gioud M., Monier JC et al. Antinuclear antibodies detected by IIF on Hep2 cells and by immunoblotting in patients with systemic sclerosis. Autoimmunity1989; 2: 165-76.
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Laemmli UKCleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature1970; 227: 680-5.
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Teng CS, Teng CT, Allfrey VGStudies of nuclear acidic proteins. J Biol Chem1971; 246: 3597-600.
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Kilianska Z. , Schmidt WN, Hnilica LSAntibodies to dehistonized chromatin of normal and fetal rat liver. CellDifferentiation1983; 13: 225-31.
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Monier JC, Perraud M., Picard F., Gioud M.Comparison of three techniques for the detection of antibodies to ds-DNA: IF on Trypanosoma gambiense, IF on Crithidia luciliae and RIA using the Farr technique. Ann Immunol (Inst Pasteur)1978; 129C: 463-74.
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Costa O., Monier JCDetection of antibodies to histones in human SLE and in murine lupus like syndromes using micro-ELISA. Ann Immunol (Inst Pasteur)1983; 134C: 365-76.
19.
Costa 0, Pinatel K, Monier JC. Antibodies to RNA detected by ELISA in SLE, SS and RA . J Rheumatol1985; 12: 928-33.
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Towbin H., Staehelin T., Gordon J.Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA1979; 76: 4350-4.
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Costa 0, Monier JC. Antihistone antibodies detected by microELISA and immunoblotting in mice with lupus like syndrome (MRL/1, MRL/n, PN and NSB strains). Clin Immunol Immunopathol1986; 40: 276-82.
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Portanova JP , Arndt RE, Tan EM, Kotzin BLAntihistone antibodies in idiopathic and drug-induced lupus recognize distinct intrahistone regions . J Immunol1987; 138: 446-51.
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Thomas JO, Wilson CM, Hardin JAThe major core histone antigenic determinants in SLE are in the trypsin-sensitive regions. FEBS1984; 169: 90-6.
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Gohill J., Cary PD, Couppez M., Fritzler MJAntibodies from patients with drug-induced and idiopathic lupus erythematosus react with epitopes restricted to the amino and carboxyl termini of histone . J Immunol1985; 135: 3116-21.
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Craft JE, Radding JA, Harding MW, Bernstein RM, Hardin JAAutoantigenic histone epitopes: a comparison between procainamide and hydralazine induced lupus. Arthritis Rheum1987; 30: 682-94.
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Bustin M., Reisch J., Einck L., Klippel JHAutoantibodies to nucleosomal proteins: antibodies to HMG-17 in autoimmune diseases. Science1982; 215: 1245-7.
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Soulard M., Barque JP, Della Valle V et al. A novel 43 kDa glycoprotein is detected in the nucleus of mammalian cells by auto-Ab from dogs with autoimmune disorders. Exp Cell Res1991; 193: 59-71.
