Canine Systemic Lupus Erythematosus. II: Antinuclear Antibodies

Abstract

The frequency and the specificities of antinuclear antibodies (ANAb) were studied in dogs with systemic lupus erythematosus (SLE) and compared to those found in normal dogs and in dogs with various infectious diseases. Whole ANAb were detected by immunofluorescence. Anti-double-stranded DNA Ab were found in only 2% of SLE dogs, whereas anti-single-stranded DNA Ab were present in 21.4% of SLE dogs and in 26.8% of dogs with infectious disease. Antihistone Ab were frequently observed in SLE dogs (71%) and are essentially directed against trypsin-resistant epitopes of H3, H4 and H2A. The Western blots of nuclear extracts of HeLa cells were recognized mainly by type 1 Ab (30%, reacting with bands of 43, 36, 35, 34, 30 and 27 kDa) and by anti-Sm Ab (12%) associated with anti-RNP Ab. Anti-SSA and anti-SSB Ab were rare.

Keywords

Systemic lupus erythematosus Antinuclear antibodies Nuclear extract Dog Immunoblot

Get full access to this article

View all access options for this article.

References

Lewis RM , Schwartz RS , Henry WB Canine systemic lupus .erythematosus. Blood 1965; 25: 143-60.

Lapras M. , Monier JC Technique d'IF appliquée au diagnostic des maladies autoimmunes chez le chien: résultats cliniques. Rev Med Vet 1971; 120: 973-89.

Monier JC , Dardenne M. , Rigal D. , Costa O. , Fournel C. , Lapras M. Clinical and laboratory features of canine lupus syndromes. Arthritis Rheum 1980; 23: 294-301.

Grinden CB , Johnson KH Systemic lupus erythematosus: literature review and report of 42 new canine cases. J Am Anim Hosp Assoc 1983; 19: 489-503.

Hubert B. , Teichner M. , Fournel C. , Monier JC Spontaneous familial SLE in a canine breeding colony. J Comp Pathol 1988; 98: 81-9.

Costa 0, Fournel E, Lotchouang JC, Monier JC, Fontaine M. Specificities of antinuclear antibodies detected in dogs with systemic lupus erythematosus. Vet Immunol Immunopathol 1984; 7: 369-82.

Brinet A. , Fournel C. , Faure JR , Venet C. , Monier JC Antihistone antibodies (ELISA and immunoblot) in canine lupus erythematosus . Clin Exp Immunol 1988; 74: 105-9.

Ait-Kaci A. , Monier JC , Mamelle N. Enzyme linked immunosorbent assay for antihistone antibodies and their presence in systemic lupus erythematosus sera. J Immunol Meth 1981; 44: 311-22.

Hardin JA , Thomas JO Antibodies to histones in systemic lupus erythematosus: localization of prominent autoantigens on histones H1 and H2B. Proc Natl Acad Sci USA 1983; 80: 7410-4.

10.

Gioud M. , Ait-Kaci A, Monier JC. Histone antibodies in systemic lupus erythematosus . Arthritis Rheum 1978; 25: 407-13.

11.

Tan EM , Cohen AS , Fries JF et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25: 1271-7.

12.

Johns EW , ed. The HMG Chromosomal Proteins . New York: Academic Press, 1982.

13.

Briolay J. , Gioud M. , Monier JC et al. Antinuclear antibodies detected by IIF on Hep2 cells and by immunoblotting in patients with systemic sclerosis. Autoimmunity 1989; 2: 165-76.

14.

Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.

15.

Teng CS , Teng CT , Allfrey VG Studies of nuclear acidic proteins. J Biol Chem 1971; 246: 3597-600.

16.

Kilianska Z. , Schmidt WN , Hnilica LS

Antibodies to dehistonized chromatin of normal and fetal rat liver. Cell

Differentiation 1983; 13: 225-31.

17.

Monier JC , Perraud M. , Picard F. , Gioud M. Comparison of three techniques for the detection of antibodies to ds-DNA: IF on Trypanosoma gambiense, IF on Crithidia luciliae and RIA using the Farr technique. Ann Immunol (Inst Pasteur) 1978; 129C: 463-74.

18.

Costa O. , Monier JC Detection of antibodies to histones in human SLE and in murine lupus like syndromes using micro-ELISA. Ann Immunol (Inst Pasteur) 1983; 134C: 365-76.

19.

Costa 0, Pinatel K, Monier JC. Antibodies to RNA detected by ELISA in SLE, SS and RA . J Rheumatol 1985; 12: 928-33.

20.

Towbin H. , Staehelin T. , Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350-4.

21.

Costa 0, Monier JC. Antihistone antibodies detected by microELISA and immunoblotting in mice with lupus like syndrome (MRL/1, MRL/n, PN and NSB strains). Clin Immunol Immunopathol 1986; 40: 276-82.

22.

Portanova JP , Arndt RE , Tan EM , Kotzin BL Antihistone antibodies in idiopathic and drug-induced lupus recognize distinct intrahistone regions . J Immunol 1987; 138: 446-5 1.

23.

Thomas JO , Wilson CM , Hardin JA The major core histone antigenic determinants in SLE are in the trypsin-sensitive regions. FEBS 1984; 169: 90-6.

24.

Gohill J. , Cary PD , Couppez M. , Fritzler MJ Antibodies from patients with drug-induced and idiopathic lupus erythematosus react with epitopes restricted to the amino and carboxyl termini of histone . J Immunol 1985; 135: 3116-21.

25.

Craft JE , Radding JA , Harding MW , Bernstein RM , Hardin JA Autoantigenic histone epitopes: a comparison between procainamide and hydralazine induced lupus. Arthritis Rheum 1987; 30: 682-94.

26.

Bustin M. , Reisch J. , Einck L. , Klippel JH Autoantibodies to nucleosomal proteins: antibodies to HMG-17 in autoimmune diseases. Science 1982; 215: 1245-7.

27.

Soulard M. , Barque JP , Della Valle V et al. A novel 43 kDa glycoprotein is detected in the nucleus of mammalian cells by auto-Ab from dogs with autoimmune disorders. Exp Cell Res 1991; 193: 59-71.