Serum Apolipoprotein H Levels in Systemic Lupus Erythematosus Are Not Influenced by Antiphospholipid Antibodies

Abstract

Anticardiolipin antibodies (aCL) were recently discovered to recognize a complex consisting of phospholipids and apolipoprotein H (apo H). In this study, we determined the serum apo H levels in 36 systemic lupus erythematosus (SLE) patients with or without antiphospholipid antibodies (aPL), including aCL and lupus anticoagulants, to clarify the possible effects of aPL on apo H levels in vivo.

The apo H levels were low in SLE patients as compared with 22 healthy controls. However, no associations were found between apo H levels and circulating aPL or clinical features of the antiphospholipid antibody syndrome. A secondary hyperlipidemic state, which probably related to lupus nephritis (proteinuria) and/or prednisolone treatment, increased apo H levels in SLE patients.

Keywords

Apolipoprotein H β2-Glycoprotein I Antiphospholipid antibodies Systemic lupus erythematosus

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References

Nimpf J. , Wurm H. , Kostner GM Interaction of β2-glycoprotein-I with human blood platelets: influence upon the ADP-induced aggregation. Thrombosis Haemostas 1985; 54: 397-401.

Schousboe I. β2-Glycoprotein I: a plasma inhibitor of the contact activation of the intrinsic blood coagulation pathway . Blood 1985; 66: 1086-91.

Nimpf J. , Bevers EM , Bomanns Phh et al. Prothrombinase activity of human platelets is inhibited by β 2-glycoprotein-I. Biochem Biophys Acta 1986; 884: 142-9.

Harris EN , Gharavi AE , Hughes Grv. Antiphospholipid antibodies. Clin Rheum Dis 1985; 11: 591-609.

Mackworth-Young C. Antiphospholipid antibodies: more than just a disease marker? Immunology Today 1990; 11: 60-5.

McNeil HP , Simpson RJ , Chesterman CN , Krilis SA Antiphospholipid antibodies are directed against a complex antigen that induces a lipid-binding inhibitor of coagulation: β2-glycoprotein I (apolipoprotein H). Proc Natl Acad Sci USA 1990; 87: 4120-4.

Galli M. , Comfurius P. , Maassen C. et al. Anticardiolipin antibodies (ACA) directed not to cardiolipin but to plasma protein cofactor. Lancet 1990 ; 335: 1544-7.

Cohnen G. Immunochemical quantitation of β2-glycoprotein I in various diseases. J Lab Clin Med 1970; 75: 212-6.

Tan EM , Cohen AS , Fries JF et al. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum 1982; 25: 1271-7.

10.

Takamatsu K. , Suehiro T. , Kawada M. , Ohno F. Clinical studies on apolipoprotein H (β 2-glycoprotein I) in patients with hyperlipoproteinemia . J Jpn Atheroscler Soc 1987; 15: 1401-8 (in Japanese).

11.

Takamatsu K. , Kishino W. , Suehiro T. , Yamano T. , Ohno F. Plasma apolipoprotein H (β2-glycoprotein I) phenotype frequencies in a Japanese population . Jpn J Human Genet 1989; 34: 279-83.

12.

Gharavi AE , Harris EN , Ascherson RA , Hughes Grv. Anticardiolipin antibodies: isotype distribution and phospholipid specificity . Ann Rheum Dis 1987; 46: 1-6.

13.

Ichikawa Y. , Kobayashi N. , Kawada T. et al. Reactivities of antiphospholipid antibodies to blood cells and their effects on platelet aggregation in vitro. Clin Exp Rheumatol 1990; 8: 461-8.

14.

Ettinger WH , Goldberg AP , Applebaum-Bowden D. , Hazzard WR Dyslipoproteinemia in systemic lupus erythematosus. Am J Med 1987; 83: 503-8.

15.

Khamashita MA , Harris EN , Gharavi AE et al. Immune mediated mechanism for thrombosis: antiphospholipid antibody binding to platelet membrane . Ann Rheum Dis 1988; 47: 849-54.

16.

McNeil HP , Chesterman CN , Krilis SA Anticardiolipin antibodies and lupus anticoagulants comprise separate antibody subgroups with different phospholipid binding characteristics. Br J Haematol 1989; 73: 506-13.

17.

Triplett DA , Brandt JT , Kaczor D. , Schaeffer J. Laboratory diagnosis of lupus inhibitors: a comparison of the tissue thromboplastin inhibition procedure with a new platelet neutralization procedure. Am J Clin Pathol 1983; 79: 678-82.

18.

Ichikawa Y. , Kobayashi N. , Kawada T. et al. Anticardiolipin antibodies by an enzyme-linked immunosorbent assay: fundamental studies on the conditions for antigen-application and specificity of the assay. Tokai J Exp Clin Med 1989; 14: 103-12.