Sage Journals: Discover world-class research

Abstract

SECMA 1® is a polypeptide purified from a green algeae of the Ulva species by several gel chromatographies, showing the following sequence (Glu-Asp-Arg-Leu-Lys-Pro). In order to determine the effect of SECMA 1® on human skin fibroblasts extracellular matrix, proteoglycans (PGs) and glycosaminoglycans (GAGs) were assayed after 24 h incubation of 20 day-old foreskin fibroblasts at the 2nd passage.

The results revealed that most of [³⁵S]sulphate was associated with fibroblast membranes, which contained (67%) of the total de novo synthesized sulphated PGs, in two distinct forms: one hydrophilic (39%), and one hydrophobic (28%). The remaining `matrix' retained 5% of proteoglycans. The remaining ³⁵S-label may represent the free label in the cytosol.

After 24 h incubation of skin fibroblasts with different concentrations of SECMA 1® (2, 4 and 10 μg/ml), the [³⁵S] sulphate incorporation into PGs of Salt-extract, sodium deoxycholate (DOC) extract and Guanidine hydrochloride (GuA-HCl)-extract was increased significantly (P<0.005) with 4 μg/ml, as compared to untreated control. The most effective concentration (4 μg/ml) increased the different [³⁵S]sulphate PGs extracts (NaCl, DOC and GuA-HCl) by respectively (66; 17 and 75%).

The relative contents of iduronic and glucuronic acid in the GAG produced by skin fibroblasts were estimated. No effect of SECMA 1® on the incorporation of [³⁵S]sulphate into Heparan sulphate was found. The incorporation of [³⁵S]sulphate into (chondroïtine sulphate + heparan sulphate) and (chondroïtine sulphate + dermatan sulphate) was increased by respectively 37% and 11% by SECMA 1® (4 μg/ml).

Keywords

SECMA 1® hexapeptide human skin fibroblasts DNA synthesis [35S]incorporation proteoglycans glycosaminoglycans

Get full access to this article

View all access options for this article.

References

Iozzo, RV. Proteoglycans and neoplasia . Cancer and Metastasis Rev 1988; 10: 39-42 .

Rapraeger A , Jalkanen M , Bernfield M. Cell surface proteoglycan associates with the cytoskeleton at the basolateral cell surface of mouse mammary epithelial cells . J Cell Biol 1986; 103: 2683-2696 .

Heinegard D , Sommarin Y. Isolation and characterization of proteoglycans . Methods Enzymol 1987; 144: 305-372 .

Cöster L , Carlstedt I , Malmström A , Särnstrand B. Biosynthesis and secretion of dermatan sulphate proteoglycans in culture of human skin fibroblasts . Biochem J 1984; 220: 575-582 .

Schmidtchen A , Carlstedt I , Malmström A , Fransson LA. Inventory of human skin fibroblast proteoglycans: Identification of multiple heparan and chondroitin/dermatan sulphate proteoglycans . Biochem J 1990; 265: 289-300 .

Höök M , Kjellen L , Johansson S , Robinson JC. Cell surface glycosaminoglycans . Annu. Rev. Biochem 1984; 53: 847-869 .

Woods A , Couchman JR , Höök M. Heparan sulphate proteoglycans of rat embryo fibroblasts . J Biol Chem 1984; 260: 10872-10879 .

Carlstedt I , Cöster L , Malmström A , Fransson LA. Proteoheparan sulphate from human skin fibroblasts. Isolation and structural characterization . J Biol Chem 1983; 258: 11629-11635 .

Cöster L et al. Structure of proteoglycan sulphates from fibroblasts. Confluent and proliferating fibroblasts produce at least three types of proteoglycan sulphates with functionally different core proteins . J Biol Chem 1986; 261: 12079-12088 .

10.

Poulsen JH , Jensen IM. D-[3H] glucosamine labelling of epidermal and dermal glycosaminoglycans in cultured human skin . J Clin Chem Clin Biochem 1988; 26: 123-128 .

11.

Lories V et al. Heparan sulphate proteoglycans of human lung fibroblasts structural heterogeneity of the core proteins of the hydrophobic cell-associated forms . J Biol Chem 1987; 262: 854-859 .

12.

Heremans A et al. Heparan sulphate proteoglycan from the extracellular matrix of human lung fibroblasts. Isolation, purification and core protein characterization . J Biol Chem 1988; 263: 4731-4739 .

13.

Bell E , Ivarsson B , Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro . Prot. Natl. Acad. Sci. USA, 1979; 76: 1274-1278 .

14.

Ennamany R et al. Effect of bolesatine, a glycoprotein from Boletus satanas Lenz on rat thymus in vivo . Toxicology 1994; 89: 113-118 .

15.

Stow JL , Sawada H , Farquhar MG. Basement membrane heparan sulphate proteoglycans are concentrated in the laminae rarae and in the podocytes of the renal glomerulus . Proc Natl Acad Sci USA 1985; 82: 3296-3300 .

16.

Cöster L et al. Biosynthesis of Dermatane Sulfate proteoglycans, the effect of β-D xyloside on the polymer modification process in fibroblasts culture . Biochem J 1991; 276: 533-539 .

17.

Stow JL , Glasgow EF , Handley CJ , Hascall VC. Biosynthesis of proteoglycans by isolated rabbit glomeruli . Arch Biochem Biophys 1983; 225: 950-957 .

18.

Linker A , Hovingh P. The heparitin sulphates (heparan sulphates) . Carbohydr Res 1973; 29: 41-62 .

19.

Schmidtchen A , Carlstedt I , Malmström A , Fransson LA. Analysis of glycosaminoglycans chains from different proteoglycan population in human embryonic skin fibroblasts . Eur J Biochem 1992; 208: 537-546 .

20.

Fedarko NS. Isolation and purification of proteoglycans . E.X.S. 1994; 70: 9-35 .

21.

Klagsbrun M , Baird A. A dual receptor system is required for basic fibroblast growth factor activity . Cell 1991; 67: 229-231 .

22.

Gallagher JT. The extended family of proteoglycans: social residents of the pericellular zone . Curr Opin Cell Biol 1989; 1: 1201-1218 .

23.

Yanagashita M , Hascall VC. Cell surface heparan sulphate proteoglycans . J Biol Chem 1992; 267: 9451-9454 .

24.

Ruoslahti E , Yamaguchi Y. Proteoglycans as modulators of growth factor activities . Cell 1991; 64: 867-869 .

25.

Klagsbrun M. The affinity of fibroblast growth factors for heparin; FGF-heparan sulphate interactions in cells and extracellular matrix . Curr Opin Cell Biol 1990; 2: 857-863 .

26.

Fantl WJ , Johnson DE , Williams LT. Signalling by receptor tyrosine kinases . Ann Rev Biochem 1993; 62: 453-481 .

SECMA 1®,a mitogenic hexapeptide from Ulva algeae modulates the production of proteoglycans and glycosaminoglycans in human foreskin fibroblast

Abstract

Keywords

Get full access to this article

References