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Abstract

1 Ricin is known to have diverse effects on the cells of different organs like liver, kidney, pancreas, intestines and parathyroid.

2 Acute decrease in serum thyroid hormone levels 24 h after ricin administration (1.5 μg/100 g) led us to suspect the toxic action of ricin on the thyroid.

3 We monitored the lipid peroxidation (LP) and anti oxidant status of the thyroid tissue to determine the role, if any, played by reactive oxygen species (ROS) in this pathology.

4 An increase of 39% in LP and 47% in superoxide dismutase, along with a 8.5% decrease in catalase

points to the imbalance in the antioxidant defence involving hydrogen peroxide and its univalent reduc tion product, the hydroxyl radical.

5 Thyroid histopathology shows destruction of thyroid follicles and necrosis, which may be due to ROS and may partly explain the 50% reduction in circulating thyroid hormones seen after ricin administration.

Keywords

reactive oxygen species antioxidants ricin thyroid lipid peroxidation free radicals

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References

Olsen S. , Phil A. Toxic lectins and related proteins. In: P Cohen and S Van Heynigens (Eds.), Molecular action of toxins and viruses. Elsevier , Amsterdam, pp. 51-105, 1982.

Endo Y. et al. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes, the site and the characteristics of the modification in 28S ribosomal RNA caused by the toxin. Journal of Biological Chemistry 1987; 262: 5908-5912.

Derenzini M. et al. Toxic effects of ricin: Studies on the pathogenesis of liver lesions. Virchows Archives of Cell Pathology 1976; 20: 15-28.

Balint GA , Karacsony G. , Varro V. The effect of ricin on parathyroid gland. Biochemical and Experimental Biology 1977; 13: 445-448.

Sadani G. , Nadkarni GD

Role of antioxidant enzyme defence in sparing rat hepatocytes from toxicity of ricin at low doses. Indian Journal of

Experimental Biology 1994; 32: 354-355.

Sadani GR , Nadkarni GD Role of tissue antioxidant defence in thyroid cancers. Cancer Letters 1996; 109: 231-235.

Sadani GR , Nadkarni GD Role of reactive oxygen species in induced hypothyroid goiters and hyperthyroidism in rats. Medical Science Research 1996; 24: 835-836.

Uchiyama M. , Mihara M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Analytical Biochemistry 1977; 86: 271-278.

Beutler E. , Duron D. , Mikus-Kelly B. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine 1963; 61: 882-886.

10.

Lowry OH et al. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 1951; 193: 265-275.

11.

Sugawara M. et al. Deficiency of superoxide dismutase in endemic goiter tissue. Journal of Endocrinology Metabolism 1988; 67: 1156-1161.

12.

Gogun Y. et al. Enhancement of SOD activity in cancer cell lines by anticancer drugs. Journal of Clinical Biochemistry and Nutrition 1990; 8: 85-92.

13.

Hafeman DG , Sunde RA , Hoekstra WG Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. Journal of Nutrition 1974; 104: 580-587.

14.

Cullings Cfa. Handbook of histological and histochemical techniques , 3rd Edn. Redwood Burn Ltd, UK, 1974.

15.

Licastro F. et al. Ricin induces the production of tumour necrosis factor-α and interleukin-1β by human peripheral-blood mononuclear cells. Biochemical Journal 1993; 294: 517-520.

16.

Nadkarni GD , Deshpande UR

Effect of ricin on acute phase response in rats. Indian Journal of

Experimental Biology 1991; 29: 584-585.

17.

Muldoon DF , Hassoun EA , Stohs S. Ricin-induced hepatic lipid peroxidation, glutathione depletion and DNA single-strand breaks in mice. Toxicon 1992; 30: 977-984.

18.

Hughes JN , Lindsay CD , Griffiths GD Morphology of ricin and albrin exposed endothelial cells is consistent with apoptotic cell death. Human and Experimental Toxicology 1996; 15: 443-451.

Reactive oxygen species involvement in ricin-induced thyroid toxicity in rat

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