Cytotoxic effects of mercuric chloride, methyl mercury, and silver lactate on polymorphonuclear leucocytes have been examined by assaying superoxide anion formation capability and chemotaxis of metal-exposed cells. Both superoxide anion formation and chemotaxis were negatively affected by all three metal compounds. Both bacteriotoxic functions were affected in a dose-dependent fashion, the functional deficits were seen at doses not affecting cell viability. Dose-response curves were remarkably similar for all three compounds. The bacteriotoxic capacity of polymorphonuclear leucocytes may be hampered by mercury and silver.
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References
1.
Christensen M., Mogensen SC & Rungby J.Toxicity and ultrastructural localization of mercuric chloride in cultured murine macrophages. Archives of Toxicology1988; 62: 440-6.
2.
Christensen M., Ellermann-Eriksen S., Rungby J., Mogensen SC & Danscher G.Histochemical and functional evaluation of mercuric chloride in cultured macrophages. Progress in Histochemistry and Cytochemistry1991 ; 23: 307-15.
3.
Rungby J., Hultman P. & Ellermann-Eriksen S.Silver affects viability and structure of cultured mouse peritoneal macrophages and peroxidative capacity of whole mouse liver. Archives of Toxicology1987; 59: 408-12.
4.
Baginski B.Effect of mercuric chloride on microbicidal activities of human polymorphonuclear leucocytes. Toxicology1988; 50: 247-56.
5.
Malamud D., Dietrich SA & Shapiro IMLow levels of mercury inhibit the respiratory burst in human polymorphonuclear leucocytes. Biochemical and Biophysical Research Communications1985; 128: 1145-51.
6.
Hultman P.Effects of inorganic mercury on the murine immune system in vivo. Linköping UniversityMedical Dissertations, No. 289, 1989 (Thesis).
7.
Obel Christiansen , N. A time course study of superoxide generation and protein kinase C activation in human neutrophils. FEBS Letters1988; 239: 195-8.
8.
Larsen CG, Andersen AO, Appella E., Oppenheim JJ & Natsushima K.The neutrophil activating protein (NAP-1) is also chemotactic for T-lymphocytes . Science1989; 243: 1464-66.
DiVincenzo GD, Giordano CJ & Schriever LSBiologic monitoring of workers exposed to silver. International Archives of Occupational and Environmental Health1985; 56: 205-15.
11.
Jensen EJ, Rungby J., Hansen JC, Schmidt E., Pedersen B. & Dahl R.Serum concentrations and accumulations of silver in skin during three months treatment with an anti-smoking chewing gum containing silver acetate . Human Toxicology1988; 7: 535-40.
12.
Rungby J.Silver-induced lipid peroxidation in mice: Interactions with selenium and nickel. Toxicology1987; 45: 135-42.
13.
Olsson GM, Rungby J., Rundquist I. & Brunk UTEvaluation of lysosomal stability in living cultured macrophages by cytofluorometry . Virchows Archiv B Cell Pathology1989; 56: 263-9.
