The purpose of this study was to evaluate the capacity of specific anti-digoxin Fab fragments to bind to and neutralize scilliroside and proscillaridin in acute poisoning. Apparent affinity constants were determined with values of 2.6 108M -1 for scilliroside and 3.8 107M-1 for proscillaridin. These results are in accordance with a possible in-vivo neutralization of these toxins.
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References
1.
Stoll A., Suter E., Kreis W. & Bussemaker BBCardiac glysosides. I. The cardiac active principles of Squill. Scillaren A. Helvetica Chimica Acta1933; 16: 703-33.
2.
Belz GG, Nubling H., Schmidt-Wiederkehr P. & Franz HEPlasmakonzentrationen und elimination von methylproscillaridin bei niereninsuffizienz . Klinische Wochenschrift1974; 52: 1078-80.
3.
Dybing F., Dybing O. & Stormorken H.The toxicity of red squill and schilliroside to rats and mice. Acta Pharmacologica et Toxicologica1952; 47: 2355a.
4.
Leck JB & Kevin Park B.A comparative study of the effects of warfarin and brodifacoum on the relationship between vitamin K1 metabolism and clotting factor activity in warfarin-susceptible and warfarin-resistant rats. Biochemical Pharmacology1981; 30: 123-8.
5.
Smith TW, Haber E., Yeatman L. & Butler VPReversal of advanced digoxin intoxication with Fab fragments of digoxin-specific antibodies. New England Journal of Medicine1976; 294: 797-800.
6.
Schemnann JM , Terrien N., Urtizberea M., Pierson P. & Denis H. Immunotoxicotherapy: Present status and future trends. ClinicalToxicology1989; 27: 1-35.
7.
Urtizberea M. , Sabouraud A., Terrien N. & Scherrmann JMImmunotoxicothérapie: stratégie dans la préparation et l'administration des anticorps spécifiques . Immunoanalyse et Biologie Spécialisée1989 ; 17: 17-25.
8.
Müller R.Calculation of average antibody affinity in antihapten sera from data obtained from radioimmunoassay. Journal of Immunological Methods1980; 34: 345-52.
9.
Chappey O. & Scherrmann JMImmunoanalyse des médicaments: Concepts et applications. Immunoanalyse et Biologie Spécialisée1989; (in press).
Urtizberea M., Latour M., Edelman L., Carcagne J., Armand J. & Scherrmann JMImmunopharmacological activity of monoclonal antidigitoxin. IABS Symposium Proceedings Utrecht1989; (in press)
