1 Hyperplastic liver nodules were induced in F-344 rats by concurrent administration of lasiocarpine (50 ppm in diet) and thioacetamide (50 mg/kg body weight twice weekly) for 15 weeks.
2 The effect of carbohydrate calorie and total calorie restriction on the fate of hyperplastic liver nodules was examined.
3 The incidence of hepatocellular carcinoma was the same in all groups of rats irrespective of the magnitude of carbohydrate calorie restriction and 50% total calorie restriction.
4 These studies demonstrate that carbohydrate or total calorie restriction has no effect on the progression of hyperplastic nodules to hepatocellular carcinoma.
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