There is compelling evidence in support of positive associations between zinc (Zn) deficiency and high accumulation of cadmium (Cd) in biological samples of different cancerous patients. The incidence of mouth cancer has been increased among people who possess habit of drinking alcohol, tobacco smoking and chewing with gradients in Pakistan. In the present study, Cd and Zn contents of blood and scalp hair samples of 92 male mouth cancer patients and 78 referents of same age group (range 35—65 years), socio-economic status, localities and dietary habits. Among referent male subjects, 56 consumed tobacco (smoking/chewing), 22 had none of smoking and chewing tobacco habits, while none of them admitted for alcohol drinking. The scalp hair and whole blood samples were oxidized by 65% nitric acid and 30% hydrogen peroxide (2:1) ratio in microwave oven. The Zn in digests was determined by flame atomic absorption while Cd was determined by electrothermal atomic absorption spectrometry. The average Cd concentration was higher, while level of Zn was lower in the blood and scalp hair samples of mouth cancer patients as compared to referent subjects (p < .001). The male referents consuming tobacco (chewing and smoking) have high level of Cd in both their biological samples as compared to those not smoking or chewing tobacco (p < .012). The Cd/Zn ratio in both biological samples was found to be higher in patients than in referent subjects.
Lippman SM, Hong WKMolecular markers of the risk of oral cancer. N Engl J Med2001; 344: 1323-1326.
2.
Oji C., Chukwuneke FNOral cancer in Enugu, Nigeria, 1998-2003. Br J Oral Maxillofacial Surg2007; 45: 298-301.
3.
Macfarlane GI , Boyle P., Evstifeeva TV, Robertson C., Scully C.Rising trends of oral cancer mortality among males worldwide: the return of an old public health problem. Cancer Causes Control1994 ; 5: 259-265.
4.
Ko YC, Huang YL, Lee CHBetel quid chewing, cigarette smoking and alcohol consumption related to oral cancer in Taiwan. J Oral Pathol Med1995; 24: 450-453.
5.
Rodu B., Jansson C.Smokeless tobacco and oral cancer: a review of the risks and determinants . Crit Rev Oral Biol Med2004; 15: 252-263.
6.
Avti PK, Kumar S., Pathak CM, Vaiphei K., Lal K.Smokeless tobacco impairs the antioxidant defense in liver, lung, and kidney of rats. Toxicol Sci2006; 89: 547-553.
7.
Lee PNCirculatory disease and smokeless tobacco in Western populations: a review of the evidence. Int J Epidemiol2007; 36: 789-804.
8.
Schildt EB, Eriksson M., Hardell L., Magnuson A.Oral snuff, smoking habits and alcohol consumption in relation to oral cancer in a Swedish case-control study. Int J Cancer1998; 77: 341-346.
9.
Muscat JE, Richie JP, Thompson S., Wynder ELGender differences in smoking and risk for oral cancer. Cancer Res1996; 56: 5192-5197.
10.
Bouquot JEReviewing oral leukoplakia. Clinical concepts for the 1990sJ Am Dent Assoc1991; 122: 80-82.
11.
Stephens WE , Calder A.Source and health implications of high toxic metal concentrations in illicit tobacco products. Environ Sci Technol2005 ; 39: 479-488.
12.
Van Wyk CW , Stander I., Padayachee A., Grobler-Rabie AFThe areca nut chewing habit and oral squamous cell carcinoma in South African Indians. A retrospective study. S Afr Med J1993; 83: 425-429.
13.
Soylak M., Saraymen R., Narin İ., DoGan M.Serum zinc levels of a poor economic and social region near an industrial zone. Trace Elements and Electrolytes1998 ; 15: 142-144.
14.
Prasad ASZinc in human health: an update. J Trace Elem Exp Med1998; 11: 63-87.
15.
Prasad AS, Beck FWJ, Doerr TD, et al. Nutritional and zinc status of head and neck cancer patients: an interpretive review. J Am Coll Nutr1998 ; 17: 409-418.
16.
Elinder CGCadmium as an environmental hazard. IARC Sci Publ1992; 118: 123-132.
17.
Goyer RAToxic and essential metal interactions. Annu Rev Nutr1997; 17: 37-50.
18.
Drasch G., Schopfer J., Schrauzer GNSelenium/ cadmium ratios in human prostates Indicators of prostate cancer risk of smokers and nonsmokers, and relevance to the cancer protective effects of selenium. Biol Trace Element Res2005; 103: 103-107.
19.
Tuzen M.Determination of some trace elements in whole blood and serum by GFAAS . Trace Elements and Electrolytes2002; 19(4):202-204.
20.
Soylak M., SaraçoGlu S., Divrikli U., Elçi L.Copper and Zinc Concentrations of Serum Samples of Healthy People Living in Tokat. Turkey. Trace Elements and Electrolytes2001;18: 47-50.
21.
Soylak M., Saraymen R., Dogan M.Investigation of Lead, Chromium, Cobalt and Molybdenum Concentrations in Hair Samples Collected from Diabetic Patients. Fresenius Environmental Bulletin1995; 4: 485-490.
22.
Ghaedi M., Shokrollahi A., Kianfar AH, Mirsadeghi AS, Pourfarokhi A., Soylak M.The determination of some heavy metals in food samples by flame atomic absorption spectrometry after their separation-preconcentration on bis salicyl aldehyde, 1,3 propan diimine (BSPDI) loaded on activated carbon. J Hazard Mater2008; 154: 128-134.
23.
Ghaedi M., Niknam K., Shokrollahi A., Niknam E., Ghaedi H., Soylak M.A solid phase extraction procedure for Fe3+, Cu2+ and Zn2+ Ions on 2-phenyl-1H-benzo[d] imidazole loaded on Triton-X100 coated poly vinyl chloride . J Hazard Mater2008; 158: 131-136.
24.
Melek E., Tuzen M., Soylak M.Flame atomic absorption spectrometric determination of cadmium(II) and lead(II) after their solid phase extraction as dibenzyldithiocarbamate chelates on Dowex Optipore V-493.Anal Chim Acta2006; 578: 213-219.
25.
Thomas SJ, MacLennan R.Slaked lime and betel nut cancer in Papua, New Guinea. Lancet1992; 340: 577-578.
26.
Trivedi AH, Balart DB, Shah PM, et al. Carcinogenic and genotoxic effects of the tobacco substitute pan masala: present status and likely future impact on the Indian population . Canc Treat Rev1996; 22: 345-354.
27.
Zowczak M., Iskra M., Paszkowski J., Manczak M., Torlinski L.Oxidase activity of ceruloplasmin and concentrations of copper and zinc in serum of cancer patients. J Trace Elem Med Biol2001; 15: 193-196.
28.
Cornelis R. , Heinzow B., Herber RFM.Sample collection guidelines for trace elements in blood and urine. Pure Appl Chem1995; 67: 1575-608.
29.
Kubova J., Hanakova V., Medved J., Stresko V.Determination of lead and cadmium in human hair by atomic-absorption spectrometric procedures after solid-phase extraction. Anal Chim Acta1997; 337: 329-334.
30.
Afridi HI, Kazi TG, Jamali MK, Kazi GH, Arain MB, Jalbani N., Shar GQAnalysis of heavy metals in scalp hair samples of hypertensive patients by conventional and microwave digestion methods. Spectrosc Lett2006; 39: 203-214.
31.
Kazi TG, Afridi HI, Kazi GH, Jamali MK, Arain MB, Jalbani N.Evaluation of essential and toxic metals by ultrasound-assisted acid leaching from scalp hair samples of children with macular degeneration patients . Clin Chim Acta2006; 369: 52-60.
32.
Kazi TG, Afridi HI, Kazi N., et al. Distribution of zinc, copper and iron in biological samples of Pakistani myocardial infarction (1st, 2nd and 3rd heart attack) patients and controls. Clin Chim Acta2008; 389: 114-119.
33.
Asha G., Vijayalakshmi D., Kumar V.Effect of intervention on the nutritional status of selected cancer patients . J Hum Ecol2004; 16: 189-192.
34.
Yadav JS, Chadha P.Genotoxic studies in pan masala chewers: a high cancer risk group . Int J Hum Genet2002; 2(2): 107-112.
35.
Newcomb PA, Carbone PPThe health consequences of smoking. Cancer Med Clin N Am1992; 76: 305-331.
36.
Ahluwalia KPAssessing the oral cancer risk of South-Asian immigrants in New York City . Cancer2005 ; 104: 2959-2961.
37.
Tomatis L.The IARC monographs program: changing attitudes towards public health . Int J Occup Environ Health2002; 8: 144-152.
38.
Warnakulasuriya S., Trivedy C., Peters TJAreca nut use: an independent risk factor for oral cancer. BMJ2002; 324: 799-800.
39.
Rivadeneira DE , Evoy D., Fahey TJ, Lieberman MD, Daly JMNutritional support of the cancer patient . CA Cancer J Clin1998; 48: 69-80.
40.
Oneschuk D. , Younus J.Natural health products and cancer chemotherapy and radiation therapy. Oncol Rev2008; 1: 233-242.
41.
Spitz MR, Santella RM, Boffetta P., Buffler P.Subgroup report: head and neck cancer. IARC Sci Publ2004; 157: p.41-47.
42.
Franceschi S., Levi F., Vecchia CL, et al. Comparison of the effect of smoking and alcohol drinking between oral and pharyngeal cancer. Int J Cancer1999; 83: 1-4.
43.
Grasso P., Mann AHSmokeless tobacco and oral cancer: an assessment of evidence derived from laboratory animals. Food Chem Toxicol1998 ; 36: 1015-1029.
44.
Chen CL, Chi CW, Liu TYHydroxyl radical formation and oxidative DNA damage induced by areca quid in vivo. J Toxicol Environ Health2002 ; 65: 327-336.
45.
Yoshida T., Tuder RMPathobiology of cigarette smoke-induced chronic obstructive pulmonary disease . Physiol Rev2007; 87: 1047-1082.
46.
Maier H., Born IA, Veith S., Adler D., Seitz HKThe effect of chronic ethanol consumption on salivary gland morphology and function in the rat. Alcoholism. Clin Exper Res1986; 10: 425-427.
47.
Csalari J., Szantai K.Transfer rate of cadmium, lead, zinc and iron from the tobacco-cut of the most popular Hungarian cigarette brands to the combustion products . Acta Aliment2002; 31: 279-288.
48.
Hecht SSTobacco carcinogens, their biomarkers and tobacco-induced cancer. Nat Rev Cancer2003; 3: 733-744.
49.
Kazi TG, Jalbani N., Arain MB, et al. Toxic metals distribution in different components of Pakistani and imported cigarettes by electrothermal atomic absorption spectrometer. J Hazard Mater2009; 163: 302-307.
50.
Sharma G., Sandhir R., Nath R., Gill K.Effect of ethanol on cadmium uptake and metabolism of zinc and copper in rats exposed to cadmium. J Nutr1991; 121: 87-91.
51.
Franceschi S., Barra S., Vecchia CL, Bidoli E., Negri E.Risk factors for cancer of the tongue and the mouth. A case-control study from northern Italy . Cancer1992; 70: 2227-2233.
52.
Brzoska MM, Moniuszko-Jakoniuk J., Jurczuk M., Chraniuk M.The influence of cadmium on bone tissue in rats. Pol J Environ Stud1997; 6: 29-32.
53.
Stohs SJ, Bagchi D.Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med1995; 18: 321-336.
54.
Waalkes MP, Coogan TP, Barter RAToxicological principles of metal carcinogenesis with special emphasis on cadmium. Crit Rev Toxicol1992; 22: 175-201.
Dong LH, Qiang WZ, Rong PY, Shu ZT, Zhu XX, Wang KTComparison of serum Zn, Cu and Se contents between healthy people and patients in high, middle and low incidence areas of gastric cancer of Fijians Province . World J Gastroenterol1999; 5: 84-86.
57.
Somer E.Health media of America essential guide to vitamins and minerals. New York: Harper Perennial, 1995, 32, p.139-14.
58.
Stohs SJ, Bagchi D., Hassoun E., Bagchi M.Oxidative mechanisms in the toxicity of chromium and cadmium ions . J Environ Pathol Toxicol Oncol2001; 20: 77-88.
59.
Timar J., Raso E., Paku S., Kopper L.Oral administration of trace element preparation and zinc inhibit liver metastasis of 3LL-HH murine tumor cells. Int J Mol Med1998; 2: 105-108.
60.
Ganapathy S. , Volpe SLZinc, exercise and thyroid hormone function . Crit Rev Food Sci Nutr1999; 39: 369-390.
61.
Linder MCNutritional biochemistry and metabolism. 2nd ed. New York: Elsevier Science Publishing Co. Inc. 1991, p.499-512.
62.
Newbern PM, Schrager TF, Broitman S.Esophageal carcinogenesis in the rat: zinc deficiency and alcohol effects on tumor induction. Path biology1997 ; 65: 39-45.
63.
Gupta SK, Shukla V., Vaidya MPSerum trace elements and Cu/Zn Ratio in breast cancer patients. J Surg Oncol1991; 46: 178-181.
64.
Leccia MT, Richard MJ, Favier A., Beani JCZinc protects against ultraviolet A1-induced DNA damage and apoptosis in cultured human fibroblasts. Biol Trace Elem Res1999 ; 69: 177-190.
