Immune effects and exposure to ethylenebisdithiocarbamate pesticides in re-entry workers in the Netherlands

Abstract

Ethylenebisdithiocarbamates are widely used as fungicides in agriculture. Although EBDC’s have a low acute toxicity, they are suspected to have immune effects at low doses. However, little human studies on these effects have been published. In the Netherlands, a study was conducted among pesticide exposed workers aimed at evaluating the short-term and long-term immune effects of exposure and the relation between ethylenebisdithiocarbamate and immune effects. Forty-one re-entry workers and 40 nonexposed controls were medically examined; furthermore, immune parameters were determined in blood, and all participants filled in a questionnaire regarding exposure and outcome parameters. The level of ethylenethiourea in urine was determined as indicator of exposure. No relevant adverse immune effects were found in the pesticide exposed workers compared with the nonexposed controls. Also no exposure response relationship between immune effects and ethylenebisdithiocarbamate in urine was found. This finding might be due to very low exposure levels of the re-entry work but might also be due to a lack of immunotoxicity of ethylenebisdithiocarbamate at normal exposure levels.

Keywords

epidemiology immune effects occupational exposure pesticides

References

1 Maroni M Colosio C Ferioli A Fait A . Biological monitoring of pesticide exposure: a review. Introduction. Toxicology 2000; 143: 1–118.

2 Centraal Bureau voor de Statistiek . Statline: Centraal Bureau voor de Statistiek (Netherlands Central Bureau of Statistics: statline.cbs.nl. Accessed May 17, 2006), 2003.

3 Liesivuori J Savolainen K . Dithiocarbamates. Toxicology 1994; 91: 37–42.

4 Houeto P Bindoula G Hoffman JR . Ethylenebisdithiocarbamates and ethylenethiourea: possible human health hazards. Environ Health Perspect 1995; 103: 568–573.

5 Israeli R Sculsky M Tiberin P . Acute intoxication due to exposure to maneb and zineb. A case with behavioral and central nervous system changes. Scand J Work Environ Health 1983; 9: 47–51.

6 Koizumi A Shiojima S Omiya M Nakano S Sato N Ikeda M . Acute renal failure and maneb (manganous ethylenebis[dithiocarbamate]) exposure. JAMA 1979; 242: 2583–2585.

7 IARC . IARC Monographs on the evaluation of the carcinogenic risk of chemicals to man: some carbamates, thiocarbamates and carbazides, International Agency for Research on Cancer (IARC), Lyon, France. vol. 12, 1976.

8 Colosio C Corsini E De Pascale G Fustinoni S Mammone T Visentin S Galli CL Maroni M . Results of a pilot study on immunotoxicity of pesticides in agricultural workers. ISEE 2001 (13th Conference of the International Society for Environmental Epidemiology). Garmish-Partenkirchen, Germany, 2–5 September 2001, A 97.

9 Colosio C Barcellini W Maroni M Alcini D Bersani M Cavallo D . Immunomodulatory effects of occupational exposure to mancozeb. Arch Environ Health 1996; 51: 445–451.

10.

10 Colosio C Fustinoni S Corsini E Bosetti C Birindelli S Boers D . Changes in serum markers indicative of health effects in vineyard workers following exposure to the fungicide mancozeb: an Italian study. Biomarkers 2007; 12: 574–588.

11.

11 Fustinoni S Campo L Colosio C Birindelli S Foà V . Application of gas chromatography-mass spectrometry for the determination of urinary ethylenethiourea in humans. J Chromatogr B Analyt Technol Biomed Life Sci 2005; 814: 251–258.

12.

12 Benezra C Ducombs G Sell Y Foussereau J . Plant contact dermatitis. Toronto: Decker Inc.; 1985. p. 105–149.

13.

13 Weisglas-Kuperus N Patandin S Berbers GA Sas TC Mulder PG Sauer PJ . Immunologic effects of background exposure to polychlorinated biphenyls and dioxins in Dutch preschool children. Environ Health Perspect 2000; 108: 1203–1207.

14.

14 Sleijffers A Garssen J de Gruijl FR Boland GJ van Hattum J van Vloten WA . Influence of ultraviolet B exposure on immune responses following hepatitis B vaccination in human volunteers. J Invest Dermatol 2001; 117: 1144–1150.

15.

15 Corsini E Birindelli S Fustinoni S De Paschale G Mammone T Visentin S . Immunomodulatory effects of the fungicide Mancozeb in agricultural workers. Toxicol Appl Pharmacol 2005; 208: 178–185.