In recent years, microbial platforms for biofuels and nutraceuticals have gained recognition as sustainable alternatives. This study investigates titanium dioxide nanoparticles’ effect on lipid and omega fatty acid production in Thraustochytrium sp. Both titanium dioxide and nitrogen starvation independently enhance lipid content, with a synergistic effect observed when combined. Titanium dioxide increases lipid content by 37.69% (up to 78.84%), while nitrogen starvation achieves a 34.91% enhancement (total lipid 75.92 ± 2.43%). The combined approach yields the highest lipid content at 81.48 ± 3.14%, denoting a 46.19% increase. Additionally, titanium dioxide increases omega-6 (LA by 66.95%) and omega-9 (OA by 44.98%) levels, while nitrogen starvation enhances omega-3 (mainly EPA, by 91.83%) levels. This eco-friendly strategy has implications for bioenergy and nutraceuticals, offering a cost-effective means of enhancing oleaginous microorganisms for biodiesel production. The research outcomes contribute to addressing key obstacles in cost-efficient biodiesel production, impacting the economic landscape of the biodiesel industry.
GadoreVMishraSRAhmaruzzamanM. Advances in photocatalytic biodiesel production: preparation methods, modifications and mechanisms. Fuel2024; 362: 130749.
2.
KarthikVPeriyasamySVaralakshmiV, et al.Biofuel: a prime eco-innovation for sustainability. In: Environmental sustainability of biofuels. Elsevier; 2023, pp.267–284. DOI: https://doi.org/10.1016/B978-0-323-91159-7.00006-0.
3.
GuptaABarrowCJPuriM. Multiproduct biorefinery from marine thraustochytrids towards a circular bioeconomy. Trends Biotechnol2022; 40: 448–462.
4.
LiSYangJMohamedH, et al.AMP Deaminase: a crucial regulator in nitrogen stress and lipid metabolism in Mucor circinelloides. Biochim Biophys Acta2024; 1869: 159434.
5.
ZhengBBanLNieY, et al.Sustainable production of biodiesel enabled by acid-base bifunctional ZnF2 via one-pot transformation of Koelreuteria integrifoliola oil: process optimization, kinetics study and cost analysis. J Clean Prod2024; 453: 142263.
6.
WangHZhouHYanQ, et al.Superparamagnetic nanospheres with efficient bifunctional acidic sites enable sustainable production of biodiesel from budget non-edible oils. Energy Convers Manag2023; 297: 117758.
7.
NimkerVDongCDPatelAK, et al.Nanocrystal cellulose from diverse biological sources: application and innovations. Energy Environ2024: 0958305X241251394. DOI: https://doi.org/10.1177/0958305X241251394.
8.
RyuKHKimBLeeJH. A model-based optimization of microalgal cultivation strategies for lipid production under photoautotrophic condition. Comput Chem Eng2019; 121: 57–66.
9.
SajidZKhanFZhangY. Process simulation and life cycle analysis of biodiesel production. Renew Energy2016; 85: 945–952.
10.
IEA, IRENA, UNSD, WB, WHO. Tracking SDG 7: the energy progress report 2021. United Nations, http://hdl.handle.net/10986/38016 (2021).
11.
NhamoGNhemachenaCNhamoS, et al.SDG7–ensure access to affordable, reliable, sustainable and modern energy. Emerald Publishing, 2020, pp.233–262. DOI: https://doi.org/10.1108/978-1-78973-799-820201001.
12.
DemisuDG. Biodiesel quality improvement by mixing the Azadirachta indica and Moringa stenopetala seed oil (in situ hybridisation) via transesterification process. Int J Sustain Energy2024; 43: 2305043.
13.
RenHYDaiYQKongF, et al.Enhanced microalgal growth and lipid accumulation by addition of different nanoparticles under xenon lamp illumination. Bioresour Technol2020; 297: 122409.
14.
SungYJPatelAKYuBS, et al.Sedimentation rate-based screening of oleaginous microalgae for utilization as a direct combustion fuel. Bioresour Technol2019; 293: 122045.
15.
YuZPeiHJiangL, et al.Phytohormone addition coupled with nitrogen depletion almost tripled the lipid productivities in two algae. Bioresour Technol2018; 247: 904–914.
16.
PatelAKarageorgouDRovaE, et al.An overview of potential oleaginous microorganisms and their role in biodiesel and omega-3 fatty acid-based industries. Microorganisms2020; 8: 434.
17.
RiosLFKleinBCLuzLF, et al.Nitrogen starvation for lipid accumulation in the microalga species Desmodesmus sp. Appl Biochem Biotechnol2015; 175: 469–476.
18.
ChauhanASPatelAKSinghaniaRR, et al.Fine-tuning of key parameters to enhance biomass and nutritional polyunsaturated fatty acids production from Thraustochytrium sp. Bioresour Technol2024; 394: 130252.
19.
TrivediJAgrawalDAtrayN,et al.Enhanced lipid production in Scenedesmus obliquus via nitrogen starvation in a two-stage cultivation process and evaluation for biodiesel production. Fuel2022; 316: 123418.
20.
IkaranZSuárez-AlvarezSUrretaI,et al.The effect of nitrogen limitation on the physiology and metabolism of Chlorella vulgaris var L3. Algal Res2015; 10: 134–144.
21.
AzizMMAKassimKAShokraviZ, et al.Two-stage cultivation strategy for simultaneous increases in growth rate and lipid content of microalgae: a review. Renew Sustain Energy Rev2020; 119: 109621.
22.
YuQLiuZXuH, et al.Tio2 nanoparticles promote the production of unsaturated fatty acids (UFAs) fighting against oxidative stress in Pichia pastoris. RSC Adv2015; 5: 41033–41040.
23.
LiangSXTWongLSDhanapalACTA,et al.Toxicity of metals and metallic nanoparticles on nutritional properties of microalgae. Water Air Soil Pollut2020; 231: 1–14.
24.
PushpalathaSSangeethaRAriramanS, et al.Photocatalyst (TiO2) as an enhancer: an attempt to enhance the production of carotenoids and lipids with the combined oxidative stresses in Coelastrella sp. M60. Clean Technol Environ Policy2021; 23: 41–53.
25.
ThiagarajanVPavaniMArchanaaS, et al.Diminishing bioavailability and toxicity of P25 TiO2 NPs during continuous exposure to marine algae Chlorella sp. Chemosphere2019; 233: 363–372.
26.
ChauhanASChenCWTambatVS, et al.Bioprocess engineering to produce essential polyunsaturated fatty acids from Thraustochytrium sp. Bioresour Technol2023; 383: 129209.
27.
PatelAKVadraleAPTsengYS, et al.Bioprospecting of marine microalgae from Kaohsiung Seacoast for lutein and lipid production. Bioresour Technol2022; 351: 126928.
28.
ChauhanASChenCWYadavH, et al.Assessment of thraustochytrids potential for carotenoids, terpenoids and polyunsaturated fatty acids biorefinery. J Food Sci Technol2023; 60: 1–13.
29.
WanSLiuXSunW, et al.Current advances for omics-guided process optimization of microbial manufacturing. Bioresour Bioprocess2023; 10: 30.
30.
LauZLLowSSEzeigweER, et al.A review on the diverse interactions between microalgae and nanomaterials: growth variation, photosynthetic performance and toxicity. Bioresour Technol2022; 351: 127048.
31.
StaterEPSonayAYHartC,et al.The ancillary effects of nanoparticles and their implications for nanomedicine. Nat Nanotechnol2021; 16: 1180–1194.
32.
AbomohraAEFJinWTuR, et al.Microalgal biomass production as a sustainable feedstock for biodiesel: current status and perspectives. Renew Sustain Energy Rev2017; 79: 1169–1180.
33.
GuptaABarrowCJPuriM. Omega-3 biotechnology: thraustochytrids as a novel source of omega-3 oils. Biotechnol Adv2013; 31: 799–813.
34.
YuKNChangSHParkSJ, et al.Titanium dioxide nanoparticles induce endoplasmic reticulum stress-mediated autophagic cell death via mitochondria-associated endoplasmic reticulum membrane disruption in normal lung cells. PLoS One2015; 10: e0131208.
35.
MetrykaOWasilkowskiDMrozikA. Insight into the antibacterial activity of selected metal nanoparticles and alterations within the antioxidant defence system in Escherichia coli, Bacillus cereus and Staphylococcus epidermidis. Int J Mol Sci2021; 22: 11811.
36.
SupenoMSihotangJPPanjaitanYV, et al.Room temperature esterification of high-free fatty acid feedstock into biodiesel. RSC Adv2023; 13: 33107–33113.
37.
ChauhanASChenCWTambatVS, et al.Biorefining of essential polyunsaturated fatty acids from microbial sources: current updates and prospects. Syst Microbiol Biomanuf2023; 26: 1–23.
38.
LaiMGhouriFSarwarS, et al.Modulation of metal transporters, oxidative stress and cell abnormalities by synergistic application of silicon and titanium oxide nanoparticles: a strategy for cadmium tolerance in rice. Chemosphere2023; 345: 140439.
39.
MishraSDeewanAZhaoH, et al.Nitrogen starvation causes lipid remodeling in Rhodotorula toruloides. bioRxiv. 2023-05.
40.
Castro-CosioPMonreal-EscalanteERomero-GeraldoR,et al.Natural and recombinant bioactive compounds from Schizochytrium sp.: recent advances and future prospects. Algal Res2023; 75: 103273.
41.
ChauhanASChenCWSinghaniaRR, et al.Valorizations of marigold waste for high-value products and their industrial importance: a comprehensive review. Resources2023; 11: 91.
42.
LatsosCVanHJTimmermansKR. The effect of nitrogen starvation on biomass yield and biochemical constituents of Rhodomonas sp. Front Mar Sci2020; 7: 563333.
43.
ChauhanASPatelAKChenCW, et al.Enhanced production of high-value polyunsaturated fatty acids (PUFAs) from potential thraustochytrid Aurantiochytrium sp. Bioresour Technol2023; 370: 128536.
44.
MaltsevYKulikovskiyMMaltsevaS. Nitrogen and phosphorus stress as a tool to induce lipid production in microalgae. Microb Cell Fact2023; 22: 239.
45.
ÖrdögVStirkWABálintP, et al.Changes in lipid, protein and pigment concentrations in nitrogen-stressed Chlorella minutissima cultures. J Appl Phycol2012; 24: 907–914.
46.
TanKWLinHShenH,et al.Nitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta. Sci Rep2016; 6: 37235.
