The most competent and operative use of renewable feedstock is super critical for the production of biodiesel which has increased attention worldwide pertaining to aquatic fern Azolla. Maximizing the biodiesel yield by optimizing the process parameters of the low-frequency ultrasonic energy-assisted transesterification process of Azolla oil is the need of the hour for minimizing the production cost of biodiesel. Response Surface Methodology (RSM) was applied using central composite rotatable design (CCRD) to find the best optimum reaction parameters for this transesterification process. The optimized reaction parameters arrived from the design of experiments were as following: methanol/Azolla oils molar ratio (A) = 6.49 mole/mole, KOH catalyst concentration (B) = 1.69 (weight% of oil), reactiion time (C) = 34.74 min and reaction temperature (D) = 38.87°C. The best higher theoretical predicted Azolla Fatty Acid Methyl Ester (FAME) yield was Y = 99.76% which is in well coincidence with the actual yield. The extracted Azolla biodiesel was tested for various fuel properties with standard test procedures and found to be in agreement with various Biodiesel standards and the results are promising in terms of utilizing Azolla oil as an inexhaustible and potentially economical source of biodiesel.
SorateKABhalePV. Biodiesel properties and automotive system compatibility issues. Renewable Sustainable Energy Rev2015; 41: 777–798.
2.
Wan GhazaliWNMMamatRMasjukiHH,et al.Effects of biodiesel from different feedstocks on engine performance and emissions: a review. Renewable Sustainable Energy Rev2015; 51: 585–602.
3.
NguyenTVNguyenKDHoNX,et al.Engine performance and combustion characteristics of a direct injection compression ignition engine fueled waste cooking oil synthetic diesel. International Journal of Coal Science & Technology2020; 7: 560–570.
4.
ZengDLiRWangB,et al.A review of transesterification from low-grade feedstocks for biodiesel production with supercritical methanol. Russ J Appl Chem2014; 87: 1176–1183.
5.
KobaYIshizakiA. Chemical composition of palm fiber and Its feasibility as cellulosic Raw material for sugar production. Agric Biol Chem [Internet]1990; 54: 1183–1187.
6.
Ban-KoffiLHanYW. Alcohol production from pineapple waste. World J Microbiol Biotechnol [Internet]1990; 6: 281–284.
7.
MielenzJR. Ethanol production from biomass: technology and commercialization status. Curr Opin Microbiol2001 Jun; 4: 324–329.
8.
LeeJ-WGwakK-SParkJ-Y, et al.Biological pretreatment of softwood Pinus densiflora by three white rot fungi. J Microbiol2007 Dec; 45: 485–491.
9.
StrayerRCBlakeJACraigWK. Canola and high erucic rapeseed oil as substitutes for diesel fuel: preliminary tests. J Am Oil Chem Soc [Internet]1983; 60: 1587–1592.
AdewalePDumontM-JNgadiM. Recent trends of biodiesel production from animal fat wastes and associated production techniques. Renew Sustain Energy Rev [Internet]2015; 45: 574–588.
13.
MaFClementsLDHannaMA. Biodiesel fuel from animal fat. Ancillary studies on transesterification of beef tallow. United States: N1998; 37: 3768–3771. https://doi.org/10.1021/ie980162s.
14.
XuJDeshussesMA. Fermentation of swine wastewater-derived duckweed for biohydrogen production. Int J Hydrogen Energy [Internet]2015; 40: 7028–7036.
15.
DerssehMGMelesseAMTilahunSA,et al.Chapter 19 - water hyacinth: review of its impacts on hydrology and ecosystem services—lessons for management of lake Tana. In: MelesseAMAbtewWSenayG (eds) Extreme hydrology and climate variability [internet]. Elsevier, 2019, pp.237–251. https://www.sciencedirect.com/science/article/pii/B9780128159989000191?via%3Dihub
16.
SinbuathongNSombatNMeksumpunS. Comparison of the increase in methane yield using alkali pretreatment for French weed and water lettuce prior to co-digestion. Environ Prog & Sustain Energy [Internet]2020; 39: e13361.
17.
GolzaryAHosseiniASaberM. Azolla filiculoides as a feedstock for biofuel production: cultivation condition optimization. Int J Energy Water Resour [Internet]2021; 5: 85–94.
18.
PrabakaranSMohanrajTArumugamA. Azolla pinnata methyl ester production and process optimization using a novel heterogeneous catalystRenew Energy [Internet]2021; 180: 353–371. Available from: https://doi.org/10.1016/j.renene.2021.08.073.
19.
SchenkPMThomas-HallSRStephensE, et al.Second generation biofuels: high-efficiency microalgae for biodiesel production. BioEnergy Res2008; 1: 20–43.
20.
HossainABMSSallehABoyceAN,et al.Biodiesel fuel production from algae as renewable energy. Am J Biochem Biotechnol2008; 4: 250–254.
21.
MataTMMartinsAACaetanoNS. Microalgae for biodiesel production and other applications: a review. Renew Sustain Energy Rev2010; 14: 217–232.
22.
Venkata MohanSRohitMVChiranjeeviP,et al.Heterotrophic microalgae cultivation to synergize biodiesel production with waste remediation: progress and perspectives. Bioresour Technol [Internet]2015; 184: 169–178.
23.
DixitRWasiullahxMalaviyaD, et al.Bioremediation of heavy metals from soil and aquatic environment: an overview of principles and criteria of fundamental processes. Sustainability2015; 7(2): 2189–2212.
24.
Tel-OrEForniC. Phytoremediation of hazardous toxic metals and organics by photosynthetic aquatic systems. Plant Biosyst - An Int J Deal with all Asp Plant Biol [Internet]2011; 145: 224–235.
25.
DhirBSharmilaPSaradhiPP. Potential of aquatic macrophytes for removing contaminants from the environment. Crit Rev Environ Sci Technol2009; 39: 754–781.
26.
PadmavathiammaPKAhmedMRahmanHA. Phytoremediation - A sustainable approach for contaminant remediation in arid and semi-arid regions -a review. Emirates Journal of Food and Agriculture2014; 26: 757–772.
27.
ManiDKumarC. Biotechnological advances in bioremediation of heavy metals contaminated ecosystems: an overview with special reference to phytoremediation. Int J Environ Sci Technol2014; 11: 843–872.
28.
KollahBPatraAKMohantySR. Aquatic microphylla Azolla: a perspective paradigm for sustainable agriculture, environment and global climate change. Environ Sci Pollut R2016; 23: 4358–4369.
29.
AroraASinghPK. Comparison of biomass productivity and nitrogen fixing potential of Azolla spp. Biomass Bioenerg2003; 24: 175–178.
30.
CostaMLSantosMCRCarrapicoF,et al.Azolla-Anabaena's behaviour in urban wastewater and artificial media—influence of combined nitrogen. Water Res2009; 43: 3743–3750.
31.
MuradovNTahaMMirandaAF, et al.Dual application of duckweed and azolla plants for wastewater treatment and renewable fuels and petrochemicals production. Biotechnol Biofuels2014; 7: 1.
32.
CostaMLSantosMCCarrapicoF. Biomass characterization of Azolla filiculoides grown in natural ecosystems and wastewater. Hydrobiologia1999; 415: 323–327.
33.
SinghAL. Comparative studies on different methods of Azolla utilization in rice culture. J Agric Sci1986; 107: 273–278.
34.
MirandaAFBiswasBRamkumarN, et al.Aquatic plant Azolla as the universal feedstock for biofuel production. Biotechnol Biofuels2016; 9: 221.
35.
PeiterASLinsPVSMeiliL, et al.Stirring and mixing in ethylic biodiesel production. J King Saud Univ - Sci [Internet2020; 32: 54–59.
36.
StamenkovićOSLazićMLTodorovićZB,et al.The effect of agitation intensity on alkali-catalyzed methanolysis of sunflower oil. Bioresour Technol2007 Oct; 98: 2688–2699.
37.
DarnokoDCheryanM. Kinetics of palm oil transesterification in a batch reactor. J Am Oil Chem Soc [Internet]2000; 77: 1263–1267.
38.
KomersKSkopalFStloukalR,et al.Kinetics and mechanism of the KOH - catalyzed methanolysis of rapeseed oil for biodiesel production. Eur J Lipid Sci Technol2002; 104: 728–737.
39.
WorapunIPianthongKThaiyasuitP. Optimization of biodiesel production from crude palm oil using ultrasonic irradiation assistance and response surface methodology. J Chem Technol Biotechnol2012; 87: 189–197.
40.
MehmoodTShaheenZMalikSA,et al.Utilization of waste and under-utilized Pongamia pinnata seed Oil for acquiring maximal process to Get ameliorate yield of biodiesel through response surface methodology. Springer2016; 7: 495–506.
41.
PrabakaranSMohanrajT. Optimization of biodiesel production from Azolla pinnata: investigation of CI engine characteristics using nano dosed biodiesel. J Mech Sci Technol [Internet]2021; 35: 4765–4773.
42.
MithinAASinghMFrancoPA,et al.Comparison of RSM and ANN Optimization Techniques and Modeling of Ultrasonic Energy Assisted Trans-esterification of Salviniaceae Filiculoides Oil Blended to Biodiesel. 2021;(February).
43.
RojiSSSRajREDhanarajCJ. Multivariant approach to optimize biodiesel extraction from neem oil using two-stage esterification process and its quality assessment. Energy Sources,: Part A: Recovery, Utilization, and Environmental Effects2016; 38: 1975–1981.
44.
BanerjeeNBarmanSSahaG,et al.Optimization of process parameters of biodiesel production from different kinds of feedstock. Mater Today Proc [Internet]2018; 5: 23043–23050.
45.
HasnainMAbideenZNazS,et al.Biodiesel production from new algal sources using response surface methodology and microwave application. Biomass Conversion and Biorefinery2021. https://doi.org/10.1007/s13399-021-01560-4
46.
RaviRZulkrninNSHRozhanNN, et al.Chemical composition and larvicidal activities of Azolla pinnata extracts against aedes (Diptera:culicidae). PLoS One [Internet]2018 Nov 6; 13: e0206982–e0206982.
47.
BrouwerPvan der WerfASchluepmannH,et al.Lipid yield and composition of Azolla filiculoides and the implications for biodiesel production. BioEnergy Res [Internet]2016; 9: 369–377.
48.
SalehzadehANaeemiASArastehA. Biodiesel production from Azolla filiculoides (water fern). Trop J Pharm Res2014; 13: 957–960.
49.
ManojkumarNMuthukumaranCSharmilaG. A comprehensive review on the application of response surface methodology for optimization of biodiesel production using different oil sources. Journal of King Saud University - Engineering Sciences2020. https://doi.org/10.1016/j.jksues.2020.09.012
50.
HoWWSNgHKGanS,et al.Ultrasound-assisted transesterification of refined and crude palm oils using heterogeneous palm oil mill fly ash supported calcium oxide catalystEnergy Sci Eng2015; 3: 257–269.
51.
XinDYuanfeiHHongyanZ,et al.Preparation of biodiesel on nano Ca-Mg-Al solid base catalyst UnderUltrasonic radiation in microaqueous Media. PETROCHEMICAL TECHNOLOGY.2009; 38: 1071–1075.
52.
GumahinACGalamitonJMAlleriteMJ, et al.Response surface optimization of biodiesel yield from pre-treated waste oil of rendered pork from a food processing industry. Bioresources and Bioprocessing2019; 6: 48.
53.
ThiruvenkatachariSSaravananCGEdwin GeoV,et al.Experimental investigations on the production and testing of azolla methyl esters from Azolla microphylla in a compression ignition engine. Fuel2021; 287: 119448.
54.
Jalilnejad FaliziNGüngören MadenoğluTYükselM,et al.Biodiesel production using gel-type cation exchange resin at different ionic forms. Int J Energy Res2019; 43: 2188–2199.
55.
Melvin JoseDFEdwin RajRDurga PrasadB,et al.A multi-variant approach to optimize process parameters for biodiesel extraction from rubber seed oil. Appl Energy2011; 88: 2056–2063.
56.
FloodMEGodingJCO’ConnorJB,et al.Analysis of biodiesel feedstock using GCMS and unsupervised chemometric methods. Journal of the American Oil chemists’. Society2014; 91: 1443–1452.
57.
HariramVBoseASeralathanS. Dataset on optimized biodiesel production from seeds of Vitis vinifera using ANN,: rSM and ANFIS. Data Brief2019; 25: 104298.
58.
SamuelODOkwuMOTartibuLK,et al.Modelling of Nicotiana Tabacum L. Oil biodiesel production: comparison of ANN and ANFIS. Frontiers in Energy Research2021; 8: 377. https://doi.org/10.3389/fenrg.2020.612165
59.
YatishKVLalithambaHSSureshR,et al.Optimization of scum oil biodiesel production by using response surface methodology. Process Saf Environ Prot2016; 102: 667–672.
60.
DwivediGSharmaMP. Experimental investigation on thermal stability of Pongamia biodiesel by thermogravimetric analysis. Egyptian Journal of Petroleum2016; 25: 33–38.
61.
AgrawalAChakrabortyS. A kinetic study of pyrolysis and combustion of microalgae chlorella vulgaris using thermo-gravimetric analysis. Bioresour Technol2013 Jan; 128: 72–80.
62.
GohainMDeviADekaD. Musa balbisiana colla peel as highly effective renewable heterogeneous base catalyst for biodiesel production. Ind Crops Prod2017; 109: 8–18.
63.
RossetMPerez-LopezOW. FTIR Spectroscopy analysis for monitoring biodiesel production by heterogeneous catalystVib Spectrosc [Internet]2019; 105: 102990.
MusharrafSGAhmedMAZehraN,et al.Biodiesel production from microalgal isolates of southern Pakistan and quantification of FAMEs by GC-MS/MS analysis. Chem Cent J2012; 6: 149.
HoekmanSKBrochARobbinsC,et al.Review of biodiesel composition,: properties, and specifications. Renew Sustain Energy Rev2012; 16: 143–169.
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