Dynamic analysis of viral load,T-lymphocyte subset count,γ-gt and lactate of pre-therapy and post-treatment AIDS patients

Abstract

Background

To investigate dynamic changes of viral load, T-lymphocyte subset counts, serum gamma glutamyl transferase activity and the concentration of lactate in HIV/AIDS patients before and after treatment.

Methods

The study enrolled 385 HIV/AIDS patients treated in Guangxi AIDS Clinical Treatment Center during December 2008 and March 2012. Patients were grouped according to baseline viral loads and complications. Real-time fluorescent quantitative PCR, flow cytometry, fully automatic biochemical analysis technique were used to detect viral loads, T-lymphocyte subset counts, serum gamma GT activity lactate and the dynamic changes were analysed before and after treatment in different viral load group patients with AIDS.

Result

Among the 385 AIDS patients, 171 (44.42%) were infected with HIV, 180 (46.75%) co-infected with tuberculosis and 34 (8.83%) co-infected with Penicillium marneffei. There were 330 (85.71%) patients in high-viral load group, and 55 (14.29%) patients in low-viral load group. There were no significant differences in parameters except lactate in high-viral load group before and after three months of treatment (p < 0.05). There were significant differences in CD₃⁺ and CD₄⁺ T-lymphocyte s and concentration of lactate in low-viral load group before and after three months of treatment, (p < .05). There were no significant differences in parameters except lactate in HIV infected group before and after three months of treatment, (p > 0.05). Compared with baseline data, there were no significant differences in parameters except lactate (p < 0.05) in HIV/tuberculosis co-infection group before and after three and six months of treatment. There were no significant differences in all parameters between three and six months of treatment (p < 0.05). In HIV/ P. marneffei co-infection group, CD₃⁺ and CD₄⁺ T-lymphocytes had significant changes (p < .05), and serum concentration of lactate, CD₈⁺ T-lymphocytes and serum gamma glutamyl transferase had no significant changes after three and six months treatment (p < 0.05).

Conclusion

Dynamic observation of viral loads, T-lymphocyte subset counts, serum gamma glutamyl transferase activity and the concentration of lactate in HIV/AIDS patients is clinically significant for drug selection and monitoring disease progression and curative effects, which would be worth further research.