We examined the effect of Kampo on the replication of ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) in the human embryonic fibroblast cell line MRC-5. Treatment of HCMV-infected cells with Sho-seiryu-to (SST; Xiao-Qing-Long-Tang in Chinese) resulted in the inhibition of viral replication without affecting the cell growth. SST treatment decreased the synthesis of viral DNA, but had no virucidal effect on cell-free HCMV. However, the inhibitory effect of SST on HCMV replication was ablated by anti-interferon-β (IFN-β) antibody suggesting that SST inhibits the replication of GCV-resistant HCMV through the induction of IFN-β. These results suggest that SST is a novel compund with potential as an anti-HCMV.
AmagayaSIizukaAMakinoBKuboMKomatsuYChengFCRuoTIItohT & TerasawaK (2001) General pharmacological properties of Sho-seiryu-to (TJ-19) extracts. Phytomedicine: International Journal of Phytotherapy and Phytopharmacology8: 338–347.
2.
BuergerIReefschlaegerJBenderWEckenbergPPoppAWeberOGraeperSKlenkHDRuebsamen-WaigmannH & HallenbergerS (2001) A novel nonnucleoside inhibitor specifically targets cytomegalovirus DNA maturation via the UL89 and UL56 gene products. Journal of Virology75: 9077–9086.
3.
BrittWJ (1996) Cytomegalovirus. In Fields Virology, 3rd edn, pp. 2493–2523. Edited by FieldsBNKnipeDM and HowlyPM. Philadelphia: Lippincott-Raven.
4.
FreitasVRFraser-SmithEB & MatthewsTR (1989) Increased efficacy of ganciclovir in combination with foscarnet against cytomegalovirus and herpes simplex virus type 2 in vitro and in vivo. Antiviral Research12: 205–212.
5.
FurukawaTFiorettiA & PlotkinS (1973) Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology11: 991–997.
6.
FurukawaTJisakiFSakamuroDTakegamiT & MurayamaT (1994) Detection of human cytomegalovirus genome in uterus tissue. Archives of Virology135: 265–277.
7.
GrundyJETrapmanJAllanJEShellamGR & MeliefCJ (1982) Evidence for a protective role of interferon in resistance to murine cytomegalovirus and its control by non-H-2-linked genes. Infection & Immunity37: 143–150.
8.
HaradaKEizuruYIsashikiYIharaS & MinamishimaY (1997) Genetic analysis of a clinical isolate of human cytomegalovirus exhibiting resistance against both ganciclovir and cidofovir. Archives of Virology142: 215–225.
9.
HaradaMSetaKItoOTamadaKLiTTeraoHTakenoyamaMKimuraG & NomotoK (1995) Concomitant immunity against tumor development is enhanced by the oral administration of a kampo medicine, Hochu-ekki-to (TJ-41: Bu-Zhong-Yi-Qi-Tang). Immunopharmacology & Immunotoxicology17: 687–703.
10.
HirtB (1967) Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology26: 365–369.
11.
HoM (1977) Virus infections after transplantation in man. Brief review. Archives of Virology55: 1–24.
12.
HossainMSTakimotoHHamanoSYoshidaHNinomiyaTMinamishimaYKimuraG & NomotoK (1999) Protective effects of hochu-ekki-to, a Chinese traditional herbal medicine against murine cytomegalovirus infection. Immunopharmacology41: 169–181.
13.
JacobsJPJonesCM & BailleJP (1970) Characteristics of a human diploid cell designated MRC-5. Nature227: 168–170.
14.
KawadeY (1980) An analysis of neutralization reaction of interferon by antibody: A proposal on the expression of neutralization titer. Journal of Interferon Research1: 61–70.
15.
KomatsuYTakemotoN & MaruyamaH (1986) Effect of Juzentaihoto on the anti- SRBC response in mice. Japanese Journal of Inflamation6: 405–413.
16.
LiXYTakimotoHMiuraSYoshikaiYMatsuzakiG & NomotoK (1992) Effect of a traditional Chinese medicine, bu-zhong-yi-qi-tang (Japanese name: Hochu-ekki-to) on the protection against Listeria monocytogenes infection in mice. Immunopharmacology & Immunotoxicology14: 383–402.
17.
MaruyamaHKawamuraH & TakemotoN (1988) Effect of Kampo medicines on phagocytes. Japanese Journal of Inflamation8: 65–73.
18.
McSharryJJMcDonoughAOlsonBTalaricoCDavisM & BironKK (2001) Inhibition of ganciclovir-susceptible and -resistant human cytomegalovirus clinical isolates by the benzimidazole L-riboside 1263W94. Clinical & Diagnostic Laboratory Immunology8: 1279–1281.
19.
MeyersJD (1986) Infection in bone marrow transplant recipients. The American Journal of Medicine81: 27–38.
20.
MurayamaTKunoKJisakiFObuchiMSakamuroDFurukawaTMukaidaN & MatsushimaK (1994) Enhancement human cytomegalovirus replication in a human lung fibroblast cell line by interleukin-8. Journal of Virology68: 7582–7585.
21.
MurayamaTYamaguchiNMatsunoH & EizuruY (2004) In vitro anti-cytomegalovirus activity of Kampo (Japanese herbal) medicine. Evidence-Based Complementary Alternative Medicine1: 285–289.
22.
NagaiTUrataM & YamadaH (1996) In vivo anti-influenza virus activity of Kampo (Japanese herbal) medicine “Sho-seiryu-to”-effects on aged mice, against subtypes of a viruses and B virus, and therapeutic effect. Immunopharmacology & Immunotoxicology18: 193–208.
23.
NagaiT & YamadaH (1998) In vivo anti-influenza virus activity of Kampo (Japanese herbal) medicine “sho-seiryu-to” — Stimulation of mucosal immune system and effect on allergic pulmonary inflammation model mice. Immunopharmacology & Immunotoxicology20: 267–281.
24.
OhnishiYYasumizuRFanHXLiuJTakao-LiuFKomatsuYHosoyaEGoodRA & IkeharaS (1990) Effects of juzen-taiho-toh (TJ-48), a traditional Oriental medicine, on hematopoietic recovery from radiation injury in mice. Experimental Hematology18: 18–22.
25.
OrangeJS & BironCA (1996) Characterization of early IL-12, IFN-alphabeta, and TNF effects on antiviral state and NK cell responses during murine cytomegalovirus infection. Journal of Immunology156: 4746–4756.
26.
SasakiTOkayamaAEizuruYMuraiKMatsuokaHUnoHMinamishimaYKataokaHKoonoMMatsuuraYNaoiNSawadaATachibanaN & TsubouchiH (1997) Progressive retinitis-encephalitis due to ganciclovir-resistant cytomegalovirus associated with aplastic anemia. Internal Medicine36: 375–379.
27.
ShigetaSKonnoKBabaMYokotaT & De ClercqE (1991) Comparative inhibitory effects of nucleoside analogues on different clinical isolates of human cytomegalovirus in vitro. Journal of Infectious Diseases163: 270–275.
28.
SissonsJG & CarmichaelAJ (2002) Clinical aspects and management of cytomegalovirus infection. Journal of Infection44: 78–83.
29.
WentworthBB & FrenchL (1970) Plaque assay of cytomegalovirus strains of human origin. Proceedures of the Society of Experimental Biology Medicine135: 253–258.
30.
YukawaTAKurokawaMSatoHYoshidaYKageyamaSHasegawaTNambaTImakitaMHozumiT & ShirakiK (1996) Prophylactic treatment of cytomegalovirus infection with traditional herbs. Antiviral Research32: 63–70.
31.
ZaiaJA (1993) Prevention and treatment of cytomegalovirus pneumonia in transplant recipients. Clinical Infectious Diseases17: 5392–5399.
