This study aimed to produce a composite of poly(propylene fumarate)/magnesium calcium phosphate as a substitutional implant in the treatment of trabecular bone defects. So, the effect of magnesium calcium phosphate particle size, magnesium calcium phosphate:poly(propylene fumarate) weight ratio on compressive strength, Young’s modulus, and toughness was assessed by considering effective fracture mechanisms. Micro-sized (∼30 µm) and nano-sized (∼50 nm) magnesium calcium phosphate particles were synthesized via emulsion precipitation and planetary milling methods, respectively, and added to poly(propylene fumarate) up to 20 wt.%. Compressive strength, Young’s modulus, and toughness of the composites were measured by compressive test, and effective fracture mechanisms were evaluated by imaging fracture surface. In both micro- and nano-composites, the highest compressive strength was obtained by adding 10 wt.% magnesium calcium phosphate particles, and the enhancement in nano-composite was superior to micro-one. The micrographs of fracture surface revealed different mechanisms such as crack pinning, void plastic growth, and particle cleavage. According to the results, the produced composite can be considered as a candidate for substituting hard tissue.
DreifkeMBEbraheimNAJayasuriyaAC.Investigation of potential injectable polymeric biomaterials for bone regeneration. J Biomed Mater Res A2013; 101: 2436–2447.
2.
FangCHouRZhouK, et al. Surface functionalized barium sulfate nanoparticles: controlled in situ synthesis and application in bone cement. J Mater Chem B2014; 2: 1264–1274.
3.
KapusettiGMisraNSinghV, et al. Bone cement based nanohybrid as a super biomaterial for bone healing. J Mater Chem B2014; 2: 3984–3997.
4.
GotoKTamuraJShinzatoS, et al. Bioactive bone cements containing nano-sized titania particles for use as bone substitutes. Biomaterials2005; 26: 6496–6505.
5.
HingstonJADunneNJLooneyL, et al. Effect of curing characteristics on residual stress generation in polymethyl methacrylate bone cements. Proc IMechE, Part H: J Engineering in Medicine2008; 222: 933–945.
6.
LewisG.Alternative acrylic bone cement formulations for cemented arthroplasties: present status, key issues, and future prospects. J Biomed Mater Res B Appl Biomater2008; 84: 301–319.
7.
KasperFKTanahashiKFisherJP, et al. Synthesis of poly(propylene fumarate). Nat Protoc2009; 4: 518–525.
8.
HeSTimmerMDYaszemskiMJ, et al. Synthesis of biodegradable poly(propylene fumarate) networks with poly(propylene fumarate)–diacrylate macromers as crosslinking agents and characterization of their degradation products. Polymer2001; 42: 1251–1260.
9.
FisherJPHollandTADeanD, et al. Synthesis and properties of photocross-linked poly(propylene fumarate) scaffolds. J Biomater Sci Polym Ed2001; 12: 673–687.
10.
PeterSJKimPYaskoAW, et al. Crosslinking characteristics of an injectable poly(propylene fumarate)/β-tricalcium phosphate paste and mechanical properties of the crosslinked composite for use as a biodegradable bone cement. J Biomed Mater Res1999; 44: 314–321.
11.
FisherJPDeanDMikosAG.Photocrosslinking characteristics and mechanical properties of diethyl fumarate/poly(propylene fumarate) biomaterials. Biomaterials2002; 23: 4333–4343.
12.
HeSYaszemskiMJYaskoAW, et al. Injectable biodegradable polymer composites based on poly(propylene fumarate) crosslinked with poly(ethylene glycol)-dimethacrylate. Biomaterials2000; 21: 2389–2394.
13.
ChangC-HLiaoT-CHsuY-M, et al. A poly(propylene fumarate)—Calcium phosphate based angiogenic injectable bone cement for femoral head osteonecrosis. Biomaterials2010; 31: 4048–4055.
14.
DomingosMGloriaACoelhoJ, et al. Three-dimensional printed bone scaffolds: the role of nano/micro-hydroxyapatite particles on the adhesion and differentiation of human mesenchymal stem cells. Proc IMechE, Part H: J Engineering in Medicine2017; 231: 555–564.
15.
LinLWangZZhouL, et al. The influence of prefreezing temperature on pore structure in freeze-dried β-TCP scaffolds. Proc IMechE, Part H: J Engineering in Medicine2013; 227: 50–57.
16.
ChewK-KLowK-LSharif ZeinSH, et al. Reinforcement of calcium phosphate cement with multi-walled carbon nanotubes and bovine serum albumin for injectable bone substitute applications. J Mech Behav Biomed Mater2011; 4: 331–339.
17.
LalwaniGHensleeAMFarshidB, et al. Tungsten disulfide nanotubes reinforced biodegradable polymers for bone tissue engineering. Acta Biomater2013; 9: 8365–8373.
18.
SalarianMXuWZBiesingerMC, et al. Synthesis and characterization of novel TiO 2-poly(propylene fumarate) nanocomposites for bone cementation. J Mater Chem B2014; 2: 5145–5156.
19.
CaiZ-YYangD-AZhangN, et al. Poly(propylene fumarate)/(calcium sulphate/β-tricalcium phosphate) composites: preparation, characterization and in vitro degradation. Acta Biomater2009; 5: 628–635.
20.
BohnerMGbureckUBarraletJE.Technological issues for the development of more efficient calcium phosphate bone cements: a critical assessment. Biomaterials2005; 26: 6423–6429.
21.
AlvesHLDos SantosLABergmannCP.Injectability evaluation of tricalcium phosphate bone cement. J Mater Sci Mater Med2008; 19: 2241–2246.
22.
LowKLTanSHZeinSHS, et al. Calcium phosphate-based composites as injectable bone substitute materials. J Biomed Mater Res B Appl Biomater2010; 94: 273–286.
23.
HofmannMMohammedAPerrieY, et al. High-strength resorbable brushite bone cement with controlled drug-releasing capabilities. Acta Biomater2009; 5: 43–49.
24.
ApeltDTheissFEl-WarrakAO, et al. In vivo behavior of three different injectable hydraulic calcium phosphate cements. Biomaterials2004; 25: 1439–1451.
25.
EsnaasharyMHRezaieHRKhavandiA, et al. Evaluation of setting time and compressive strength of a new bone cement precursor powder containing Mg–Na–Ca. Proc IMechE, Part H: J Engineering in Medicine2018; 232: 1017–1024.
26.
WuFWeiJGuoH, et al. Self-setting bioactive calcium–magnesium phosphate cement with high strength and degradability for bone regeneration. Acta Biomater2008; 4: 1873–1884.
27.
KlammertUReutherTBlankM, et al. Phase composition, mechanical performance and in vitro biocompatibility of hydraulic setting calcium magnesium phosphate cement. Acta Biomater2010; 6: 1529–1535.
28.
KumarRKalmodiaSNathS, et al. Phase assemblage study and cytocompatibility property of heat treated potassium magnesium phosphate–silicate ceramics. J Mater Sci Mater Med2009; 20: 1689–1695.
29.
EsnaasharyMHRezaieHRKhavandiA, et al. Solubility controlling of the precursor powders of magnesium phosphate cement by changing the powder composition. Adv Appl Ceram2017; 116: 286–292.
30.
SarkarAK.Hydration/dehydration characteristics of struvite and dittmarite pertaining to magnesium ammonium phosphate cement systems. J Mater Sci1991; 26: 2514–2518.
31.
LeeK-WWangSYaszemskiMJ, et al. Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites. Biomaterials2008; 29: 2839–2848.
32.
HeoS-JKimS-EWeiJ, et al. Fabrication and characterization of novel nano- and micro-HA/PCL composite scaffolds using a modified rapid prototyping process. J Biomed Mater Res Part A2008; 89A: 108–116.
33.
WeiJHeoSKimD, et al. Comparison of physical, chemical and cellular responses to nano- and micro-sized calcium silicate/poly(ε-caprolactone) bioactive composites. J R Soc Interface2008; 5: 617–630.
34.
WangM.Hydroxyapatite-polyethylene composites for bone substitution: effects of ceramic particle size and morphology. Biomaterials1998; 19: 2357–2366.
35.
YangKYangQLiG, et al. Mechanical properties and morphologies of polypropylene with different sizes of calcium carbonate particles. Polym Compos2006; 27: 443–450.
36.
ChanC-MWuJLiJ-X, et al. Polypropylene/calcium carbonate nanocomposites. Polymer2002; 43: 2981–2992.
37.
Wagoner JohnsonAJHerschlerBA. A review of the mechanical behavior of CaP and CaP/polymer composites for applications in bone replacement and repair. Acta Biomater2011; 7: 16–30.
38.
ZunjarraoSCSinghRP.Characterization of the fracture behavior of epoxy reinforced with nanometer and micrometer sized aluminum particles. Compos Sci Technol2006; 66: 2296–2305.
39.
JohnsenBBKinlochAJMohammedRD, et al. Toughening mechanisms of nanoparticle-modified epoxy polymers. Polymer2007; 48: 530–541.
40.
QuaresiminMSalviatoMZappalortoM.Toughening mechanisms in nanoparticle polymer composites: experimental evidences and modeling. In: QinQYeJ (eds) Toughening mechanisms in composite materials. Amsterdam: Elsevier, 2015, pp.113–133.
41.
ZuevVV.The mechanisms and mechanics of the toughening of epoxy polymers modified with fullerene C 60. Polym Eng Sci2012; 52: 2518–2522.
42.
SinghRPZhangMChanD.Toughening of a brittle thermosetting polymer: effects of reinforcement particle size and volume fraction. J Mater Sci2002; 37: 781–788.
